CYTOTOXIC ACTIVITY OF ALKALOID EXTRACTS OF DIFFERENT PLANTS AGAINST BREAST CANCER CELL LINE


Farah A Al-marzook, Rabab Omran

Abstract


Objectives: To study in vitro cytotoxic activity of total alkaloid extracts of Pinus sabiniana L., Phoenix dactylifera L. and Ferocactus sp. L. against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and non-tumorigenic fetal hepatic cell line (WRL-68).

Methods: Plant powder of each P. sabiniana L. leaves, P. dactylifera L. pollen grains, and Ferocactus sp. L. The leaves were extracted separately with 80% methanol, chloroform at pH 2 and pH 10 and the chloroform portion was dried to obtain the total alkaloid extracts. The total alkaloids were detected qualitatively by Mayer’s, Dragendorff’s and Hager’s reagents and estimated quantitatively by bromocresol green spectrophotometry depending on the atropine calibration curve. The cytotoxic activity was evaluated by 3-[4, 5-dimethylthiazoyl]-2, 5-diphenyltetrazolium bromide assay.

Results: The extract of P. sabiniana L. had highest total alkaloid content (164.62±2.8 mg/100 g dry weight of plant) than the other plants (P. dactylifera l., Ferocactus sp. L.), the total alkaloids of Ferocactus sp. L. and P. dactylifera L., reduced the cell viability of both cell lines, the highest reduction occurred in the concentration 400 μg/ml was 46±2.20% (MCF-7) and 56.2±2.2% (WRL-68) for Ferocactus sp. L., followed by 56.2±2.2% (MCF-7) and 57.5±3.2% (WRL-68) for P. dactylifera L. The alkaloids of P. sabiniana was very lower effects on both cell lines MCF-7, and WRL-68 was 89.3±3.44% and 90.16±2.7%, respectively, at the same concentration.

Conclusion: Plant alkaloids had variable effects against cancer and normal cell lines depending on the type of alkaloid compounds and their concentration in the extract.


Keywords


Alkaloids, Pinus sabiniana, Phoenix dactylifera, Ferocactus sp., In vitro, Cytotoxicity, Breast cancer.

| PDF |

References


Izevbigie EB. Discovery of water-soluble anticancer agents (edotides) from a vegetable found in Benin City, Nigeria. Exp Biol Med (Maywood) 2003;228(3):293-8.

Desai AG1, Qazi GN, Ganju RK, El-Tamer M, Singh J, Saxena AK, et al. Medicinal plants and cancer chemoprevention. Curr Drug Metab 2008;9:581-91.

Verma N, Jha K, Chaudhary S, Singh O, Kumar A. Phytochemistry, pharmacology and traditional uses of Leptadenia pyrotechnica - An important medicinal plant. Indian J Pharm Biol Res 2014;2(1):128-34.

Oksman-Caldentey KM, Inzé D. Plant cell factories in the post-genomic era: New ways to produce designer secondary metabolites. Trends Plant Sci 2004;9(9):433-40.

Capasso A, Aquino R, Tommasi ND, Piacente S, Rastrelli L, Pizza C. Neuropharmacology activity of alkaloids from South American medicinal plants. Curr Medi Chem CNS Agents 2002;2:1-15.

Huang M, Gao H, Chen Y. Chimmitecan, a novel 9-substituted camptothecin, with improved anticancer pharmacologic profiles in vitro and in vivo. J Clin Cancer Res 2007;13(4):1298-307.

Li W, Shao Y, Hu L, Zhang X, Chen Y, Tong L, et al. BM6, a new semi-synthetic vinca alkaloid, exhibits its potent in vivo anti-tumor activities via its high binding affinity for tubulin and improved pharmacokinetic profiles. J Cancer Biol Ther 2007;6(5):787-94. AQ3 8. Wang ZT, Liang GY. Zhong Yao Hua Xue. Shanghai Sci Tech 2009;31(23):1987-91.

Harbone JB. Phytochemical methods. A Guide to Modern Techniques of Plant Analysis. 2nd ed. London: Chapman and Hall; 1984. p. 307.

Harborne JB. Phytochemical Methods. 2nd ed. New York: Chapman and Hall; 1984. p. 288.

Antherden LM. Text Book of Pharmaceutical Chemistry. 8th ed. London: Oxford University Press; 1969. p. 813-4.

Neelima N, Gajanan N, Sudhakar M, Kiran V. A preliminary phytochemical investigation on the leaves of Solanum xanthocarpum. Int J Res Ayurveda Pharm 2011;2(3):845-50.

Shamsa F, Monsef H, Ghamooshi R, Verdian-Rizi M. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci 2008;32:17-20.

Amanlou M, Khosravian P, Effat S, Akbari HM. Determination of buprenorphine in raw material and pharmaceutical products using ion-pair formation. Bull Korean Chem Soc 2007;28(2):183-90.

Liu CP, Tsai WJ, Lin YL, Liao JF, Chen CF, Kuo YC. The extracts from Nelumbo Nucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells. Life Sci 2004;75(6):699-16.

Levesque R, SPSS. Programming and Data Management: A Guide for SPSS and SAS Users. 4th ed. Chicago: SPSS Inc.; 2007.

Al-Samarai AH, Al-Salihi FG, Al-Samarai RR. Phytochemical constituents and nutrient evaluation of date palm (Phoenix dactylifera, L.) Pollen grains. Tikrit J Pure Sci 2016;21(1):56-62.

Sun Y, Xun K, Wang Y, Chen X. A systematic review of the anticancer properties of berberine, a natural product from Chinese herbs. Anticancer Drugs 2009;20(9):757-69.

Sakai T, Ohno N, Sakai H, Hyuga T. Extraction-spectrophotometric determination of berberine in crude drugs by the formation of a new ion associate. Anal Sci 1991;7:39-43.

Fadhil S, Reza MH, Rouhollah G,Reza VR. Spectrophotometric determination of total alkaloids in Peganum harmala L. using bromocresol green. J Pharm 2010;4:275-8.

Debnath B, Uddin MD, Patari P, Das M, Maiti D, Manna K. Estimation of alkaloids and phenolics of five edible cucurbitaceous plants and their antibacterial activity. Int J Pharm Pharm Sci 2015;7(12):223-7.

Fazel S, Monsef H, Ghamooshi R, Verdian-Rizi M. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci 2008;32:17-20.

Keum YS, Jeong WS, Kong AN. Chemoprevention byisothiocyanates and their underlying molecular signaling mechanisms. Mutat Res 2004;555(1-2):191-202.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39(1):44-84.

Yu S, Kong AN. Targeting carcinogen metabolism by dietary cancer preventive compounds. Curr Cancer Drug Targets 2007;7(5):416-24.

Sharma R, Chandan G, Chahal A, Saini RV. Antioxidant and anticancer activity of methanolic extract from Stephania elegans. Int J Pharm Pharm Sci 2017;9(2):245-9.

Saleh MR, Al-Ataby SM, Al-Samarray YS. The cytotoxicity effect of ethanolic crude extract of Cnicus benedictus L. leaves on the murine mammary adencarcinoma cell line AMN-3. Iraqi J Cancer Med Genet 2015;8(1):72-8.

Haris M, Mahmood R, Rahman H, Rahman N. In vitro cytotoxic activity of Clerodendrum infortunatum L. Against T47D, PC-3, A549 and HCT-116 human cancer cell lines and its phytochemical screening. Int J Pharm Pharm Sci 2016;8:439-44.

Lu JJ, Bao JL, Chen XP, Huang M, Wang YT. Alkaloids isolated from natural herbs as the anticancer agents. Evid Based Complement Alternat Med 2012;2012:12.

Dogra RK, Khanna S, Shanker R. Immunotoxicological effects of piperine in mice. Toxicology 2004;196:229-36.

Unchern S, Saito H, Nishiyama N. Selective cytotoxicity of piperine on cultured rat hippocampal neurons in comparison with cultured astrocytes: The possible involvement of lipid peroxidation. Biol Pharm Bull 1997;20(9):958-61.

Choy CS, Cheah KP, Chiou HY, Li JS, Liu YH, Yong SF, et al. Induction of hepatotoxicity by sanguinarine is associated with oxidation of protein thiols and disturbance of mitochondrial respiration. J Appl Toxicol 2008;28(8):945-56.

Chan WH. Embryonic toxicity of sanguinarine through apoptotic processes in mouse blastocysts. Toxicol Lett 2011;205(3):285-92.




About this article

Title

CYTOTOXIC ACTIVITY OF ALKALOID EXTRACTS OF DIFFERENT PLANTS AGAINST BREAST CANCER CELL LINE

Keywords

Alkaloids, Pinus sabiniana, Phoenix dactylifera, Ferocactus sp., In vitro, Cytotoxicity, Breast cancer.

DOI

10.22159/ajpcr.2017.v10i7.18189

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 7 July 2017 Page: 168-171

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

57 Views | 62 Downloads

Authors & Affiliations

Farah A Al-marzook
Department of Biology, College of Science, University of Babylon, Iraq
Iraq

Rabab Omran
Department of Biology, College of Science, University of Babylon, Iraq.
Iraq


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.