ETHANOLIC EXTRACTS OF HEDYOTIS CORYMBOSA L. IMPROVES MONOSODIUM IODOACETATE-INDUCE OSTEOARTHRITIS IN RAT


Anton Bahtiar, Fitri Arum Sari, Mega Audina Putri, Natasha Linsie Corona Datunsolang, Ade Arsianti

Abstract


ABSTRACT
Objective: The objective of this study is to explore the effects of the 70% ethanolic extract of pearl grass on the immune system of the osteoarthritis
model rat, characterized by the number of leukocytes and lymphocytes, and the histology of the joint. Osteoarthritis is a degenerative disease
characterized by chronic inflammation in the joints. Based on the daily practice of herbal medicine in some community in Indonesia, pearl grass
usually used for anti-inflammation but not a lot of data to support it.
Methods: We used 36 male rats Sprague-Dawley strain divided into 6 groups. Normal group was given 0.5% of CMC, the negative control group was given
0.025 ml of sodium iodoacetate in 0.9% saline, the positive groups control group was given a suspension of glucosamine-chondroitin 135 mg/200 g bb,
three were given pearls grass extract in various dose 5.625 mg, 11.25 mg, and 22.5 mg, respectively. 28 days after sodium iodoacetate induction, the extracts
were given orally once daily for 21 days. Measurement of inflammation of knee joint and the number of leukocytes and lymphocytes were counted on day
14th, 28th, and 49th after sodium iodoacetate induction. After treatment, all rats were sacrified and all knee joints were collected to subject for histology.
Results: The results showed that the extract of pearl grass in all doses was able to decrease the number of leukocytes and lymphocytes significantly
and prevent proteoglycan degradation. The results showed that the extract of pearl grass with a given dose variations have antiinflammation effect
and been able to protect proteoglycan significantly.
Conclusion: Doses 3 (22.5 mg/200 g BW) is the best result. These results indicate that pearl grass can be further investigated as a treatment for osteoarthritis.
Keyword: Osteoarthritis, Sodium iodoacetate, Pearl grass, Hedyotis corymbosa L. Lamk., Immune system.

 


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About this article

Title

ETHANOLIC EXTRACTS OF HEDYOTIS CORYMBOSA L. IMPROVES MONOSODIUM IODOACETATE-INDUCE OSTEOARTHRITIS IN RAT

DOI

10.22159/ajpcr.2017.v10i3.16558

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 473-476

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

107 Views | 147 Downloads

Authors & Affiliations

Anton Bahtiar
Universitas Indonesia
Indonesia

Fitri Arum Sari

Mega Audina Putri

Natasha Linsie Corona Datunsolang

Ade Arsianti


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