ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS


Vinueza D, LÓpez E, Acosta K, Abdo S

Abstract


Objective: The aim of this study was to evaluate anti-inflammatory activity and cytotoxicity in vitro of hydroalcoholic extract of Bidens andicola.

Methods: B. andicola hydroalcoholic extract was obtained from aerial parts of B. andicola, following a standardized methodology. Briefly, aerial parts of B. andicola were extracted with ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions in a rotary evaporator, and finally the residue was freeze-drying to obtain the hydroalcoholic extract of B. andicola. Anti-inflammatory activity and cytotoxicity assays were carried out using in vitro isolated neutrophils model using stable water-soluble tetrazolium salts.

Results and Conclusions: The in vitro anti-inflammatory assay on isolated neutrophils demonstrated that the hydroalcoholic extract showed antiinflammatory
activity compared to aspirin, with inflammatory inhibition percent values of 80.138±0.729 to hydroalcoholic extract of B. andicola and 82.117±0.762 to aspirin, each tested in five replicates at the concentration of 200 ppm of hydroalcoholic extract or reference.

 


Keywords


Anti-inflammatory, Cytotoxicity, Bidens andicola, Cell proliferation reagent, Water-soluble tetrazolium salts.

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References


Wu J, Wan Z, Yi J, Wu Y, Peng W, Wu J. Investigation of the extracts from Bidens pilosa Linn. var. Radiata Sch. Bip. For antioxidant activities and cytotoxicity against human tumor cells. J Nat Med 2013;67(1):17-26.

Kviecinski MR, Felipe KB, Schoenfelder T, de Lemos Wiese LP, Rossi MH, Gonçalez E, et al. Study of the antitumor potential of Bidens pilosa (Asteraceae) used in Brazilian folk medicine. J Ethnopharmacol 2008;117(1):69-75.

Hilgert NI. Plants used in home medicine in the Zenta River basin, Northwest Argentina. J Ethnopharmacol 2001;76(1):11-34.

Rehecho S, Uriarte-Pueyo I, Calvo J, Vivas LA, Calvo MI. Ethnopharmacological survey of medicinal plants in Nor-Yauyos, a part of the Landscape Reserve Nor-Yauyos-Cochas, Peru. J Ethnopharmacol 2011;133(1):75-85.

De-la-Cruz H, Vilcapoma G, Zevallos PA. Ethnobotanical study of medicinal plants used by the Andean people of Canta, Lima, Peru. J Ethnopharmacol 2007;111(2):284-94.

De Tommasi N, Piacente S, Pizza C. Flavonol and chalcone esterglycosides from Bidens andicola. J Nat Prod 1998;61(8):973-7.

Li J, Mottamal M, Li H, Liu K, Zhu F, Cho YY, et al. Quercetin-3-methyl ether suppresses proliferation of mouse epidermal JB6 P cells by targeting ERKs. Carcinogenesis 2012;33(2):459-65.

Tseng HL, Li CJ, Huang LH, Chen CY, Tsai CH, Lin CN, et al. Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway. Toxicol Appl Pharmacol 2012;264(1):104-13.

Andersen OM, Markham KR. Flavonoids: Chemistry, biochemistry and applications. Angewandte Chemie International Edition. Vol. 45. Boca Raton: Verlag Chemie; 2006. p. 1237.

Evans WW. Evans-Trease and Evans Pharmacognosy. 15th ed. Edinburgh: Saunders; 2010.

Boukhris M, Simmonds MS, Sayadi S, Bouaziz M. Chemical composition and biological activities of polar extracts and essential oil of rose-scented geranium, Pelargonium graveolens. Phytother Res 2013;27(8):1206-13.

Siddiqui RA, English D, Harvey K, Cui Y, Martin MI, Wentland J, et al. Phorbol ester-induced priming of superoxide generation by phosphatidic acid-stimulated neutrophils and granule-free neutrophil cytoplasts. J Leukoc Biol 1995;58(2):189-95.

Choudhary MI, Azizuddin, Jalil S, Atta-ur-Rahman. Bioactive phenolic compounds from a medicinal lichen, Usnea longissima. Phytochemistry 2005;66(19):2346-50.

Berridge MV, Tan AN, Mccoy KD, Wang RU. The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts. Biochemica 1996;4:4-9. Available from: https://www.google.com. ec/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact= 8&ved=0ahUKEwjyvoLpw_7RAhUL02MKHVI2AfEQFggdMAA &url=https%3A%2F%2Fshop.roche.com%2Fwcsstore%2FRASCata logAssetStore%2FArticles%2FBIOCHEMICA_96_4_p14-19.pdf& usg=AFQjCNGw-cSecaV6Owi0WhPDzbb4bCrN3g.

Tan AS, Berridge MV. Superoxide produced by activated neutrophils efficiently reduces the tetrazolium salt, WST-1 to produce a soluble formazan: A simple colorimetric assay for measuring respiratory burst activation and for screening anti-inflammatory agents. J Immunol Methods 2000;238(1-2):59-68.

MacDonald TM, Beard K, Bruppacher R, Hasford J, Lewis M, Logan RF, et al. The safety of drugs for OTC use: What evidence is required for an NSAID switch? Pharmacoepidemiol Drug Saf 2002;11(7):577-84.

Martin C, Connelly A, Keku TO, Mountcastle SB, Galanko J, Woosley JT, et al. Nonsteroidal anti-inflammatory drugs, apoptosis, and colorectal adenomas. Gastroenterology 2002;123(6):1770-7.

Zahara K, Bibi Y, Tabassum S, Bashir T, Haider S, Araa A, et al. A review on pharmacological properties of Bidens biternata: A potential nutraceutical. Asian Pac J Trop Dis 2015;5(8):595-9. Available from: http://www.sciencedirect.com/science/article/pii/S2222180815608945.

Yuan LP, Chen FH, Ling L, Dou PF, Bo H, Zhong MM, et al. Protective effects of total flavonoids of Bidens pilosa L. (TFB) on animal liver injury and liver fibrosis. J Ethnopharmacol 2008;116(3):539-46.

Fidrianny I, Nadia E, Ruslan WK. In vitro antioxidant activities, total flavonoid, phenolic and carotenoid content from various extracts of four species Asteraceae herb. Int J Pharm Pharm Sci 2015;7(4):192-7. Available from: http://www.innovareacademics.in/journals/index.php/ijpps/article/view/4626/8576.

Shin KY, Won BY, Ha HJ, Yun YS, Chang KA, Lee HG, et al. Ultrafiltrated fraction of Korean red ginseng extract improves memory impairment of tg2576 mice via inhibition of soluble AB production and acetylcholinesterase activity. Int J Pharm Pharm Sci 2016;8(4):272-7. Available from: http://www.innovareacademics.in/journals/index.php/ijpps/article/view/10684/4929.

Brandão MG, Krettli AU, Soares LS, Nery CG, Marinuzzi HC. Antimalarial activity of extracts and fractions from Bidens pilosa and other Bidens species (Asteraceae) correlated with the presence of acetylene and flavonoid compounds. J Ethnopharmacol 1997;57(2):131-8.

Bairwa K, Kumar R, Sharma RJ, Roy RK. An updated review on Bidens pilosa L. Der Pharm Chem 2010;2(3):325-37. Available from: http://www.search.ebscohost.com/login.aspx?direct=true&db=aph&AN=73827797&site=ehost-live.

Zulueta MC, Tada M, Ragasa CY. A diterpene from Bidens pilosa. Phytochemistry 1995;38(6):1449-50. Available from: http://www.sciencedirect.com/science/article/pii/003194229400793S.

Hollman PC, de Vries JH, van Leeuwen SD, Mengelers MJ, Katan MB. Absorption of dietary quercetin glycosides and quercetin in healthy ileostomy volunteers. Am J Clin Nutr 1995;62(6):1276-82.

Karuppagounder V, Arumugam S, Thandavarayan RA, Sreedhar R, Giridharan VV, Watanabe K. Molecular targets of quercetin with anti-inflammatory properties in atopic dermatitis. Drug Discov Today 2016;21(4):632-9.




About this article

Title

ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS

Keywords

Anti-inflammatory, Cytotoxicity, Bidens andicola, Cell proliferation reagent, Water-soluble tetrazolium salts.

DOI

10.22159/ajpcr.2017.v10i6.17574

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 160-163

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

70 Views | 28 Downloads

Authors & Affiliations

Vinueza D
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,
Ecuador

LÓpez E
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,
Ecuador

Acosta K
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,
Ecuador

Abdo S
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,


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