Vinueza D, Cajamarca D, Acosta K, Pilco G


Objective: The aim of this research was to evaluate the photoprotective effect of Oreocallis grandiflora hydroalcoholic extract (OGHE) against
ultraviolet (UV)B-induced cell death model on a strain of Escherichia coli (ATCC 25922) and to determine the sun protection factor (SPF) using the
equation proposed by Mansur.
Methods: OGHE was obtained from leaves of O. grandiflora, following a standardized methodology. In short, O. grandiflora leaves were extracted with
ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions through rotary evaporator, and
finally, the residue was freeze drying to obtain OGHE. The photoprotective effect was carried out using in vitro UVB-induced cell death model on a
strain of E. coli (ATCC 25922), like a first approach to study its potential application on cosmetics.
Results and Conclusions: From results, O. grandiflora is an important resource to produce new cosmetic products. However, the safety of OGHE is
necessary to a rational development in that sense. OGHE shows advantages in relation to conventional active compounds of commercial sunscreens used
in this research (2-ethylhexyl 4-methoxycinnamate and 2-ethylhexyl 4-(dimethylamino)benzoate) at the concentration of 2 mg/mL, on survivor time
(with OGHE until 120 min), range of inactivation of E. coli caused by UVB (OGHE K value minor against to positive controls), and high SPF (13.56±0.21).



Photoprotective, Ultraviolet-B, Oreocallis grandiflora, Ultraviolet-B-induced cell death, Escherichia coli.

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Applied Sciences; Medicine and Health Sciences


Photoprotective, Ultraviolet-B, Oreocallis grandiflora, Ultraviolet-B-induced cell death, Escherichia coli.





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Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 2 February 2018 Page: 276-280

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Authors & Affiliations

Vinueza D
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.

Cajamarca D
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.

Acosta K
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.

Pilco G
Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.

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