EVALUATION OF ANTIMICROBIAL POTENTIAL OF DIFFERENT SOLVENT EXTRACTS OF SOME MEDICINAL PLANTS OF SEMI-ARID REGION

  • Hemali Padalia Department of Biosciences (UGC-CAS), Phytochemical, Pharmacological and Microbiological Laboratory, Saurashtra University, Rajkot, Gujarat, India.
  • Tejas Rathod Department of Biosciences (UGC-CAS), Phytochemical, Pharmacological and Microbiological Laboratory, Saurashtra University, Rajkot, Gujarat, India.
  • Sumitra Chanda Department of Biosciences (UGC-CAS), Phytochemical, Pharmacological and Microbiological Laboratory, Saurashtra University, Rajkot, Gujarat, India.

Abstract

 

 Objective: Infections caused by microorganisms that have become resistant to commonly used antibiotics have become a major nuisance globally. The problem of microbial resistance is increasing rapidly, and therefore there is an urgent need to develop novel antimicrobial agents from a natural source. The aim of the present study was to evaluate the antimicrobial activity of different solvent extracts of aerial part of Alysicarpus procumbens, Fimbristylis dichotoma, Saccharum spontaneum, Suaeda nigra, and Typha angustifolia against pathogenic microorganisms.

Methods: The extraction was done by individual cold percolation method using five solvents of different polarity, viz., petroleum ether, ethyl acetate, acetone, methanol, and water (aqueous). The antimicrobial activity was done by agar well diffusion method against Gram-positive and Gram-negative bacteria and fungi.

Results: All the plant solvent extracts showed varied level of antimicrobial activity against different microorganisms. All extracts of five plants showed better antibacterial activity than antifungal activity; Gram-positive bacteria were more susceptible than Gram-negative bacteria.

Conclusion: The polarity of solvent greatly influences extractive yield and antimicrobial activity of medicinal plants. The best activity was shown by solvent extracts of S. nigra. Hence, it can be considered as good source of antimicrobial agents.

Keywords: Medicinal plants, Antimicrobial activity, Extractive yield, Solvent extracts.

References

1. Rathod MC, Dhale DA. Pharmacognostic characterization and phytochemical screening of Launaea procumbens. Int J Pharm Res Sch 2013;3(1):41-50.
2. Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant extract against plant and human pathogens. World J Agric Sci 2008;4(5):839-43.
3. Regis AS, Gabriel JJ. Antifungal activity of Cyanotis axillaris (L.) D. Don ex sweet against opportunistic fungal strains. Int J Pharm Pharm Sci 2017;9(7):140-3.
4. Kusmardiyani S, Novita G, Fidrianny I. Antioxidant activities from various extracts of different parts of kelakai (Stenochlaena palustris) grown in central Kalimantan - Indonesia. Asian J Pharm Clin Res 2016;9(2):215-9.
5. Lad H, Dixit D, Joshi A, Bhatnagar D. Antioxidant and anti-inflammatory effects of Vitex negundo on freund’s complete adjuvant induced arthritis. Int J Pharm Pharm Sci 2015;7(1):81-5.
6. Ahmad R, Raja V, Sharma M. Hepatoprotective activity of ethyl acetate extract of Adhatoda vasica in swiss albino rats. Int J Curr Res Rev 2013;5(6):16-21.
7. Khan MF, Khan ZI, Uddin RM, Rahman MS, Rashid MA. In vivo hypoglycemic and alloxan induced antidiabetic activity of Xeromphis uliginosa Retz. Afr J Pharm Pharmacol 2015;9(11):363-6.
8. Ghate NB, Hazra B, Sarkar R, Mandal N. In vitro anticancer activity of Spondias pinnata bark on human lung and breast carcinoma. Cytotechnology 2014;66(2):209-18.
9. Selvamohan T, Ramadas VS, Shibila SK. Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. Adv Appl Sci Res 2012;3(5):3374-81.
10. Dean DA, Burchard KW. Fungal infection in surgical patients. Am J Surg 1996;171(3):374-82.
11. Graybill JR. Histoplasmosis and AIDS. J Infect Dis 1988;158(3):623-6.
12. Sieradzki K, Roberts RB, Haber SW, Tomasz A. The development of vancomycin resistance in a patient with methicillin-resistant Staphylococcus aureus infection. N Engl J Med 1999;340(7):517-23.
13. Amer WM, Abouwarda AM, El Garf IA, Dawoud GT, Abdelmohsen G. Phytochemical composition of Solanum elaeagnifolium Cav. and its antibacterial activity. J Biol Pharm Appl Sci 2013;2(6):1282-306.
14. Parekh J, Chanda S. In vitro antibacterial activity of the crude methanol extract of Woodfordia fructicosa kurz. flower (Lythraceae). Braz J Microbiol 2007;38:204-7.
15. Perez C, Paul M, Bazerque P. An antibiotic assay by the agar well diffusion method. Acta Biol Med Exp 1993;15:113-5.
16. Chanda S, Rakholiya K, Parekh J. Antimicrobial efficacy of Mesua ferrea L. seed extracted in different solvents against infection causing pathogenic strains. J Acute Dis 2013;2(4):277-81.
17. Fernández-Agullo A, Pereira E, Freire MS, Valentao P, Andradec PB, Gonzalez-Alvareza J, et al. Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Ind Crops Prod 2013;42:126-32.
18. Chanda S, Parekh J, Baravalia Y, Parekh S. Antimicrobail and antioxidant efficacy of various solvent extracts of seeds and fruits rind of Caesaipinia pulcherrima Swartz. Arch Clin Microbiol 2010;1:1-7.
19. Al-Farsi M, Lee CY. Optimization of phenolics and dietary fibre extraction from date seeds. Food Chem 2008;108(3):977-85.
20. Ammar I, Ennouria M, Atti H. Phenolic content and antioxidant activity of cactus (Opuntiaficus-indica L.) flowers are modified according to the extraction method. Ind Crops Prod 2015;64:97-104.
21. Kumar GD, Tulasi CD, Ramakrishnaiah G. Phytochemical screening and evaluation of in vitro antimicrobial activity of Drosera spatulata Var. Bakoensis-an indigenous carnivorous plant against respiratory tract infectious microbes. Asian J Pharm Clin Res 2016;9(6):274-83.
22. Padalia H, Chanda S. Comparative phytochemical analysis of aerial parts of A. procumbeans, F. dichotoma, S. sponteneum, S. nigra and T. angustifolia. J Phcog Phytochem 2015;4(2):11-6.
23. Burt S. Essential oils: Their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol 2004;94(3):223-53.
24. Kavak DD, Altiok E, Bayraktar O, Ulku S. Pistacia terebinthus extract: As a potential antioxidant, antimicrobial, and possible [3-glucuronidase] inhibitor. J Mol Catal B Enzym 2010;64(3):167-1.
25. Padalia H, Chanda S. Antimicrobial efficacy of different solvent extracts of Tagetes erecta L. flower, alone and in combination with antibiotics. Appl Microbiol 2015;1(1):1-10.
26. Nouri L, Nafchi AM, Karim AA. Phytochemical, antioxidant, antibacterial, and amylase inhibitory properties of different extracts from betel leaves. Ind Crops Prod 2014;62:47-52.
27. Morales-Cabrera M, Hernández-Morales J, Leyva-Rúelas G, Salinas-Moreno Y, Soto-Rojas L, Castro-Rosas J. Influence of variety and extraction solvent on antibacterial activity of Roselle (Hibiscus sabdariffa L.) calyxes. J Med Plants Res 2013;7(31):2319-2.
Statistics
393 Views | 420 Downloads
Citatons
How to Cite
Padalia, H., T. Rathod, and S. Chanda. “EVALUATION OF ANTIMICROBIAL POTENTIAL OF DIFFERENT SOLVENT EXTRACTS OF SOME MEDICINAL PLANTS OF SEMI-ARID REGION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 11, Nov. 2017, pp. 295-9, doi:10.22159/ajpcr.2017.v10i11.17662.
Section
Original Article(s)