ANTIPROLIFERATIVE AND DNA CLEAVAGE ACTIVITY OF VARIOUS SOLVENT EXTRACTS OF ACACIA FARNESIANA LINN. POD


Soul Shekhar Sp, Manjulatha K, Satyanarayan Nd

Abstract


Objective: Cancer has become the major disease by uncontrolled cell growth effecting large population on the globe. This study, deals with antiproliferative activity of different solvent extracts, viz., n-hexane (S1), dichloromethane (DCM) (S2), and methanol (S3) of Acacia farnesiana pod on four cancer cell lines, viz., chronic myelogenous leukemia (K562), breast cancer (MCF-7), hepatocellular carcinoma (HePG2), colorectal adenocarcinoma (Colo 205), and DNA cleavage activity of the extracts on CT-DNA.

Methods: The antiproliferative study was performed by MTT assay and DNA cleavage studies of the solvent extracts (S1, S2, and S3) and its fractions (S4 and S5) was carried out by agarose gel electrophoresis method.

Results: The antiproliferative activity results revealed that n-hexane extract (S1) has showed activity against MCF-7 (21.70 %) cell line and methanol extract (S3) against K562 (24.5%) and HePG2 (23.3%) cell lines. The DNA cleavage could be seen at every concentration tested by n-hexane (S1), DCM (S2), and methanol (S3) extracts and significant cleavage was observed at concentrations of 25, 50, and 100 μg by fractions (S4) and (S5) of methanol extract.

Conclusion: The results indicated that the extract of A. farnesiana pod (n-hexane and methanol) has antiproliferative properties and the DNA cleavage studies performed on CT-DNA was found that the extracts and its fractions showed significant activity at the concentrations tested.


Keywords


MTT assay, CT-DNA, Ethidium bromide, Agarose gel electrophoresis, Leukemia, MCF-7 cell line, K562 cell line, HePG2 cell line.

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About this article

Title

ANTIPROLIFERATIVE AND DNA CLEAVAGE ACTIVITY OF VARIOUS SOLVENT EXTRACTS OF ACACIA FARNESIANA LINN. POD

Keywords

MTT assay, CT-DNA, Ethidium bromide, Agarose gel electrophoresis, Leukemia, MCF-7 cell line, K562 cell line, HePG2 cell line.

DOI

10.22159/ajpcr.2017.v10i9.19548

Date

01-09-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 9 September 2017 Page: 197-201

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Soul Shekhar Sp
Department of Pharmaceutical Chemistry, Kuvempu University, Postgraduate Centre, Kadur, Karnataka, India.
India

Manjulatha K
Department of Biochemistry, School of Life Science, University of Hyderabad, Gachibowli, Hyderabad, Telangana, India.
India

Satyanarayan Nd
Department of Pharmaceutical Chemistry, Kuvempu University, Postgraduate Centre, Kadur, Karnataka, India.
India


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