AMELIORATIVE EFFECT OF SIDA CORDIFOLIA L. AGAINST SCOPOLAMINE-INDUCED COGNITIVE DEFICIT MICE MODEL
Keywords:Scopolamine, Amnesia, Nil, Sida cordifolia L, Transfer latency, Step down latency
Â Objective: In this study, ameliorative effect of aqueous extracts of Sida cordifolia and hydroethanolic extracts of S. cordifolia L. (AESC and EESC, respectively) was evaluated against scopolamine (Sco)-induced amnesia in mice. S. cordifolia L. is a well-know Ayurvedic plant which has been administered anciently for various nervous disorders including loss of memory. Female Swiss albino mice were used for Sco-induced amnesia model and acute toxicity study.
Methods: Acute toxicity study was carried out to select safe and effective doses (50, 100 and 250 mg/kg; p.o.) of AESC and EESC for further pharmacological evaluation. The AESC and EESC at selected doses and donepezil (Dpl) (5 mg/kg; p.o.) were administered to different groups for consecutive 15 days. On 15th day of treatment, Sco (0.4 mg/kg, intraperitoneally) was administered to different groups for induction of amnesia. Mice were subjected to transfer latency (TL) and step down latency (SDL) tests on the 16th day to evaluate the effect of AESC and EESC on memory.
Results: Sco treatment caused a significant increase in TL and decrease in SDL. The EESC significantly and dose dependently reversed the Sco-induced amnesia. The maximum effect was seen in EESC (100 mg/kg) treated group. This effect of EESC was comparable to the group treated with Dpl. The group treated with varying doses of AESC showed no significant effect on TL and SDL.
Conclusion: These findings suggested the possible therapeutic potential of EESC (70%) for diseases related to memory dysfunctions like dementia in Alzheimer's disease.
McCarty MF. Toward prevention of Alzheimers disease-potential nutraceutical strategies for suppressing the production of amyloid beta peptides. Med Hypotheses 2006;67(4):682-97.
Korczyn AD, Vakhapova V. The prevention of the dementia epidemic. J Neurol Sci 2007;257:2-4.
Ferreira A, ProenÃ§a C, Serralheiro ML, AraÃºjo ME. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J Ethnopharmacol 2006;108(1):31-7.
Ellis JM. Cholinesterase inhibitors in the treatment of dementia. J Am Osteopath Assoc 2005;105(3):145-58.
Rang HP, Dale MM, Ritter JM, Flower RJ, Henderson G. Rang and Daleâ€™s Pharmacology. 7th ed. London: Elsevier; 2012.
Arun Raj GR, Shailaja U, Debnath P, Banerjee S, Rao PN. Exploratory studies on the therapeutic effects of kumarabharana rasa in the management of chronic tonsillitis among children at a tertiary care hospital of Karnataka. J Tradit Complement Med 2015;6(1):29-33.
Vaidya AB, Rajagopalan TG, Mankodi NA, Antarkar DS, Tathed PS, Purohit AV, et al. Treatment of Parkinsonâ€™s disease with the cowhage plant-Mucuna pruriens Bak. Neurol India 1978;26(4):171-6.
Bhattacharya SK, Kumar A, Ghosal S. Effects of glycowithanolides from Withania somnifera on an animal model of Alzheimerâ€™s disease and perturbed central cholinergic markers of cognition in rats. Phyther Res 1995;9(2):110-3.
Pattar PV, Jayaraj M. Pharmacognostic and phytochemical investigation of Sida cordifolia L. A threatened medicinal herb. Int J Pharm Pharm Sci 2012;4(1):114-7.
Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F, et al. Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. J Ethnopharmacol 2003;84:131-8.
Swathy SS, Panicker S, Nithya RS, Anuja MM, Rejitha S, Indira M. Antiperoxidative and antiinflammatory effect of Sida cordifolia Linn. On quinolinic acid induced neurotoxicity. Neurochem Res 2010;35(9):1361-7.
Rastogi R, Malhotra B. Compendium of Indian Medicinal Plants. 4th ed; 1985. Available from: http://www.vedicbooks.net/compendium-indian-medicinal-plants-volumes-p-13632.html. [Last accessed on 2017 Mar 29].
Nagashayana N, Sankarankutty P, Nampoothiri MR, Mohan PK, Mohanakumar KP. Association of L-DOPA with recovery following Ayurveda medication in Parkinsonâ€™s disease. J Neurol Sci 2000;176(2):124-7.
Khurana N, Sharma N, Patil S, Gajbhiye A. Phyto-pharmacological properties of Sida cordifolia: A review of folklore use and pharmacological activities. Asian J Pharm Clin Res 2016;9(14):52.
Khurana N, Gajbhiye A. Ameliorative effect of Sida cordifolia in rotenone induced oxidative stress model of Parkinsonâ€™s disease. Neurotoxicology 2013;39:57-64.
Khurana N, Jain PK, Pounikar Y, Shailendra P, Gajbhiye A. Reversal of reserpine-induced orofacial dyskinesia and catalepsy by Sida cordifolia. Int J Pharmacol Pharm Technol 2012;1(2):121-5.
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983;54(4):275-87.
Habibyar AF, Sharma N, Khurana N. PASS assisted prediction and pharmacological evaluation of hesperidin against scopolamine induced amnesia in mice. Eur J Pharmacol 2016;789:385-94.
Khurana N, Ishar MP, Gajbhiye A, Goel RK. PASS assisted prediction and pharmacological evaluation of novel nicotinic analogs for nootropic activity in mice. Eur J Pharmacol 2011;662:22-30.
Vasudevan M, Parle M. Memory enhancing activity of Anwala churna (Emblica officinalis Gaertn.): An Ayurvedic preparation. Physiol Behav 2007;91(1):46-54.
Franzotti EM, Santos CV, Rodrigues HM, MourÃ£o RH, Andrade MR, Antoniolli AR. Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva-branca). J Ethnopharmacol 2000;72:273-7.
Rao K, Mishra S. Antihepatotoxic activity of Sida cordifolia whole plant. Fitoterapia 1998;69(1):20-3.
Franco CI, Morais LC, Quintans-JÃºnior LJ, Almeida RN, Antoniolli AR. CNS pharmacological effects of the hydroalcoholic extract of Sida cordifolia L. leaves. J Ethnopharmacol 2005;98(3):275-9.
Asdaq SM, Nayeem N, Das AK. Effect of hydroalcholic extracts of Sida cordifolia L. on lipid profile in rats. Pharmacol Online 2008;3:227-39.
Dhingra D, Parle M, Kulkarni SK. Memory enhancing activity of Glycyrrhiza glabra in mice. J Ethnopharmacol 2004;91:361-5.
Itoh J, Nabeshima T, Kameyama T. Utility of an elevated plus-maze for the evaluation of memory in mice: Effects of nootropics, scopolamine and electroconvulsive shock. Psychopharmacology (Berl) 1990;101(1):27-33.
Luo J, Yin JH, Wu HZ, Wei Q. Extract from fructus cannabis activating calcineurin improved learning and memory in mice with chemical drug-induced dysmnesia. Acta Pharmacol Sin 2003;24(11):1137-42.
Giovannini MG, Casamenti F, Bartolini L, Pepeu G. The brain cholinergic system as a target of cognition enhancers. Behav Brain Res 1997;83:1-5.
Huff FJ, Mickel SF, Corkin S, Growdon JH. Cognitive functions affected by scopolamine in Alzheimerâ€™s disease and normal aging. Drug Dev Res 1988;9:12271-8.
Kanwal A, Mehla J, Kuncha M, Ganga V, Naidu M, Gupta YK, et al. Anti-amnesic activity of Vitex negundo in scopolamine induced amnesia in rats. Pharmacol Pharm 2010;14236:1-8.
Mishima K, Tsukikawa H, Miura I, Inada K, Abe K, Matsumoto Y, et al. Ameliorative effect of NC-1900, a new AVP4-9 analog, through vasopressin V1A receptor on scopolamine-induced impairments of spatial memory in the eight-arm radial maze. Neuropharmacology 2003;44(4):541-2. 32. Rubaj A, Zgodzinski W, Sieklucka-Dziuba M. The influence of adenosine A3 receptor agonist: IB-MECA, on scopolamine- and MK- 801-induced memory impairment. Behav Brain Res 2003;141(1):11-7.
Shete RV, Bodhankar SL. Neuropharmacology of ethanolic extract of Hemidesmus indicus. Electron J Pharmacol Ther 2009;2:63-70.
Une HD, Sarveiya VP, Pal SC, Kasture VS, Kasture SB. Nootropic and anxiolytic activity of saponins of Albizzia lebbeck leaves. Pharmacol Biochem Behav 2001;69:439-4.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.