• Jyotsana Sharma Department of Pharmacognosy, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
  • Navneet Khurana Department of Pharmacognosy, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
  • Neha Sharma Department of Pharmacognosy, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
  • Rakesh Garg Department of Pharmacognosy, S.D. College of Pharmacy, Barnala, Punjab, India



 Objective: In the existing study, Ocimum tenuiflorum was assessed for its antioxidant activity. This activity was assessed using the 2,2-diphenyl-1- picrylhydrazyl (DPPH) method. The drug was identified and standardized on the basis of organoleptic, negligible characters and its quantitative physicochemical standards.

Methods: Preliminary phytochemical screening was also carried out to examine out the presence of various phytoconstituents. It revealed the presence of alkaloids, phytosterols, resin, flavonoids, tannins, diterpenes, and protein in the seed extract. DPPH method was done used for evaluating the antioxidant activity.

Results: It has been observed that different concentrations (100, 200, 300, 400, and 500 μg/mL) used had shown 82.1%, 83.4%, 86.6%, 89.1%, and 93.2% of cell inhibition, respectively.

Conclusion: Thus, the present study on pharmacognostic standardization, physicochemical evaluation and in vitro antioxidant activity of O. tenuiflorum seed would be useful to supplement information in regard to its in vivo evaluation for different pathological conditions.

Keywords: Ocimum tenuiflorum, Lamiaceae, Standardization, Phytochemical screening, Antioxidant.

Author Biography

Jyotsana Sharma, Department of Pharmacognosy, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
School of Pharmaceutical Sciences, Asst. Prof


1. Farnsworth NR, Morris RW. Higher plants-the sleeping giant of drug development. Am J Pharm Sci Support Public Health 1976;148(2):46-52.
2. De Smet PA. The role of plant-derived drugs and herbal medicines in healthcare. Drugs 1997;54(6):801-40.
3. Quick J, Zhang X, Kasilo O, D’Alessio R, Land S, Graaff P, et al. WHO Policy Perspectives on Medicines-Traditional Medicine-Growing Needs and Potential. A. MPHT a. P. Cluster. Geneva: WHO; 2002. p. 1-6.
4. Ghosh GR. Tulasi (NO Labiatae, genus-Ocimum). N Approaches Med Health 1995;3:23-9.
5. Simpson BB, Conner OM. Economic Botany Plants in Our World. Hamburg: McGraw Hill Book Company; 1986. p. 640.
6. Sanda K, Koba K, Nambo P, Gaset A. Chemical investigation of Ocimum species growing in Togo. Flavour Fragr J 1998;13(4):226-32.
7. Singh S, Taneja M, Majumdar DK. Biological activities of Ocimum sanctum L. Fixed oil-an overview. Indian J Exp Biol 2007;45(5):403-12.
8. Naquvi KJ, Dohare SL, Shuaib M, Ahmad MI. Chemical composition of volatile oil of Ocimum sanctum Linn. Int J Biomed Adv Res 2012;3(2):129-31.
9. Vani SR, Cheng SF, Chuah CH. Comparative study of volatile compounds from genus Ocimum. Am J Appl Sci 2009;6(3):523.
10. Khan A, Ahmad A, Akhtar F, Yousuf S, Xess I, Khan LA, et al. Ocimum sanctum essential oil and its active principles exert their antifungal activity by disrupting ergosterol biosynthesis and membrane integrity. Res Microbiol 2010;161(10):816-23.
11. Anbarasu K, Vijayalakshmi G. Improved shelf life of protein-rich tofu using Ocimum sanctum (Tulsi) extracts to benefit Indian rural population. J Food Sci 2007;72(8):M300-5.
12. Mondal S, Mirdha BR, Mahapatra SC. The science behind sacredness of Tulsi (Ocimum sanctum Linn.). Indian J Physiol Pharmacol 2009;53(4):291-306.
13. Aguiyi JC, Obi CI, Gang SS, Igweh AC. Hypoglycaemic activity of Ocimum gratissimum in rats. Fitoterapia 2000;71(4):444-6.
14. Interaminense LF, Jucá DM, Magalhães PJ, Leal-Cardoso JH, Duarte GP, Lahlou S. Pharmacological evidence of calcium-channel blockade by essential oil of Ocimum gratissimum and its main constituent, eugenol, in isolated aortic rings from DOCA-salt hypertensive rats. Fundam Clin Pharmacol 2007;21(5):497-506.
15. Freire CM, Marques MO, Costa M. Effects of seasonal variation on the central nervous system activity of Ocimum gratissimum L. Essential oil. J Ethnopharmacol 2006;105(1-2):161-6.
16. Ifesan BO, Ijarotimi OS, Oshundahunshi OF. Evaluation of the antioxidant activity of Ocimum sp. J Food Technol 2006;4(4):318-21.
17. Pessoa LM, Morais SM, Bevilaqua CM, Luciano JH. Anthelmintic activity of essential oil of Ocimum gratissimum Linn. And eugenol against Haemonchus contortus. Vet Parasitol 2002;109(1-2):59-63.
18. Madeira SV, Matos FJ, Leal-Cardoso JH, Criddle DN. Relaxant effects of the essential oil of Ocimum gratissimum on isolated ileum of the guinea pig. J Ethnopharmacol 2002;81(2):1-4.
19. Mishra P, Mishra S. Study of antibacterial activity of Ocimum sanctum extract against gram positive and gram negative bacteria. Am J Food Technol 2011;6(4):336-41.
20. Monga J, Sharma M, Tailor N, Ganesh N. Antimelanoma and radioprotective activity of alcoholic aqueous extract of different species of Ocimum in C(57)BL mice. Pharm Biol 2011;49(4):428-36.
21. Jeeva S, Sukumaran S, Joselin J, Geetha VS. Phytochemical and antimicrobial study of the flowers of Tulsi an endemic palm of Southern Western Ghats. Med Aromat Plants 2015;4:181.
22. Prakash P, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: A short review. Indian J Physiol Pharmacol 2005;49(2):125-31.
23. Sharma KR. Solar aided combined cycle power plant. J Laser Opt Photonics 2014;1:e103.
24. Singh V, Verma O. Ocimum sanctum (Tulsi): Bio-pharmacological activities. Pharmacology 2010;1(10):WMC001046.
25. Otto WJ, Holówko WH, Krawczyk MS, Król MA, Wilkowojska UM, Sierdzinski J, et al. Hcc angiogenic propriety and tumor recurrence in liver transplant recipients. Pharm Anal Acta 2015;6:343.
26. Kabir Dar A, Siddiqui MA, Wahid-Ul H, Lone AH, Manzoor N, Haji A. Threat status of rheum emodi-a study in selected cis-himalayan regions of Kashmir valley Jammu and Kashmir India. Med Aromat Plants 2015;4(183):2167-412.
27. Geeta, Vasudevan DM, Kedlaya R, Deepa S, Ballal M. Activity of Ocimum sanctum (the traditional Indian medicinal plant) against the enteric pathogens. Indian J Med Sci 2001;55(8):434-8, 472.
28. Eddouks M, Hebi M, Zeggwagh N, El-Bouhali B, Hajji LH. Effect of Momordica charantia, Camellia sinensis and Cinnamon species on insulin resistance. Med Aromat Plants 2015;4(182):2167-412.
29. Rehman HU. In vitro propagation of Kainth (Pyrus pashia) using explants from forced cutting. J Hortic 2015;127:1-6.
30. Ciurea SO, Kongtim P, Rondon G, Chen J, Tomuleasa C, Konopleva M, et al. Should a more personalized approach be applied to hematopoietic stem-cell transplantation? J Stem Cell Res Ther 2015;5:272.
31. Joseph BA, Nair VM. Ocimum sanctum Linn. (Holy basil): Pharmacology behind its anti-cancerous effect. Int J Pharm Bio Sci 2013;4(2):556-75.
32. Chanda S, Nagani K. In vitro and in vivo methods for anticancer activity evaluation and some Indian medicinal plants possessing anticancer properties: An overview. J Pharmacogn Phytochem 2013;2(2):2856.
33. Symons MC, Gutteridge JM. Free Radicals and Iron: Chemistry, Biology, and Medicine. Oxford, UK: Oxford University Press; 1998.
34. Halliwell B, Aruoma OI. DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems. FEBS Lett 1991;281(1-2):9-19.
35. Kokate CK, Purohit AP, Gokhle SB. Textbook of Pharmacognosy. Pune: Nirali Prakashan; 2001. p. 533-7.
36. Ansari SH. Essentials of Pharmacognosy. 1st ed., Vol. 576. New Delhi: Birlia Publication; 2005. p. 580-1.
37. Mukherjee PK. Quality Control of Herbal Drugs. 1st ed. New Delhi: Business Horizons; 2002. p. 110048.
38. Trease GE, Evan WC. Textbook of Pharmacognosy. London: Balliere Tindall; 1985.
39. Sharma VK, Tiwari M, Chauhan NS, Nema RK. Phytochemical investigation on the ethanolic extract on the leaves of Zizyphus xylopyrus (Retz.) Willd. Int J Agron Plant Prod 2012;3(1):26-37.
40. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37(8):911-7.
41. European Committee for Standardization. European Standard EN14104. Acid Value was Determined by Tritrimetry Method. Brussels, Belgium: European Committee for Standardization; 2003.
42. Hookey GJ. Standard Test method for Density and Relative density of liquids by digital Density Meter.
43. Brandariz I, Barriada JL, Vilarino T, de Vicente ME. Comparison of several calibration procedures for glass electrodes in proton concentration. Monatsh Chem Chem Mon 2004;135(12):1475-88.
44. Hae-Ryong P. Protective effects of Paeonia Lactiflora Pall on Hydrogen Peroxide-induced Apoptosis in PC12 cells. Afr J Biotechnol 2006;5(23):2388-96.
430 Views | 945 Downloads
How to Cite
Sharma, J., N. Khurana, N. Sharma, and R. Garg. “PHYTOCHEMICAL EVALUATION AND ANTIOXIDANT SCREENING STUDIES OF OCIMUM TENUIFLORUM LINN SEEDS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 16, Sept. 2017, pp. 76-82, doi:10.22159/ajpcr.2017.v10s4.21341.
Original Article(s)