CARDIOPROTECTIVE EFFECT OF INM 176 ON ISCHEMIA REPERFUSION INJURY USING RAT HEART MODEL
Keywords:Angelica gigas, INM-176, Ischemia, Reperfusion, Nil, Myocardial injury
Objective: Cardioprotective effect of INM-176 in ischemia reperfusion (I/R) injury in rat heart was evaluated by measuring the extent of release of the enzyme lactate dehydrogenase (LDH) and creatine phosphokinase (CK) and by measuring the myocardial infarction (MI) size.
Methods: Five groups of animals were employed. Animals were sacrificed, and heart was mounted over the Langendorff's apparatus. Group I was employed as control group, Group II was the myocardial ischemic (MI) group, and Group III, IV, and V were pre-treated with different doses of INM- 176 (150, 300, and 600 mg/kg). After this, global ischemia accompanied by reperfusion for 30 and 120 minutes was given, respectively. The effluent was collected from coronary vessel before the initiation of ischemia, immediately, after 5 and 30 minutes of reperfusion. The magnitude of myocardial injury was measured by LDH and CK release in coronary effluent. MI size was also evaluated macroscopically using triphenyl tetrazolium chloride staining.
Results: In the present study, INM-176 was found to produce a dose-dependent cardioprotective effect against I/R injury, as observed by a decrease in the level of LDH, CK, and infarct size. Pre-treatment with a dose of 600 mg/kg was found to be more effective as compared to 150 and 300 mg/kg dose of INM-176.
Conclusion: INM-176 pre-treatment at a dose of 600 mg/kg provides cardioprotection against I/R-induced myocardial injury.
Zhou T, Chuang CC, Zuo L. Molecular characterization of reactive oxygen species in myocardial ischemia-reperfusion injury. Biomed Res 2015;2015:1-9.
Kalogeris T, Bao Y, Korthuis RJ. Mitochondrial reactive oxygen species: A double edged sword in ischemia/reperfusion versus preconditioning. Redox Biol 2014;2:702-14.
Amani M, Jeddi S, Ahmadiasl N, Usefzade N, Zaman J. Effect of HEMADO on level of CK-MB and LDH enzymes after ischemia/ reperfusion injury in isolated rat heart. Bioimpacts 2013;3(2):101-4.
Kalogeris T, Baines CP, Krenz M, Korthuis RJ. Cell biology of ischemia/reperfusion injury. Int Rev Cell Mol Biol 2012;298:229-317.
Kaminski KA, Bonda TA, Korecki J, Musial WJ. Oxidative stress and neutrophil activation-the two keystones of ischemia/reperfusion injury. Int J Cardiol 2002;86(1):41-59.
Eltzschig HK, Collard CD. Vascular ischaemia and reperfusion injury. Br Med Bull 2004;70:71-86.
Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern Med 2008;8:63.
Chan PH. Reactive oxygen radicals in signaling and damage in the ischemic brain. J Cereb Blood Flow Metab 2001;21(1):2-14.
Hou X, Han J, Yuan C, Ren H, Zhang Y, Zhang T, et al. Cardioprotective effects of total flavonoids extracted from xinjiang sprig Rosa rugosa against acute ischemia/reperfusion-induced myocardial injury in isolated rat heart. Cardiovasc Toxicol 2015;16:54-66.
Park SJ, Jung HJ, Son MS, Jung JM, Kim DH, Jung IH, et al. Neuroprotective effects of INM-176 against lipopolysaccharide-induced neuronal injury. Pharmacol Biochem Behav 2012;101(3):427-33.
Park SJ, Jung JM, Lee HE, Lee YW, Kim DH, Kim JM, et al. The memory ameliorating effects of INM-176, an ethanolic extract of Angelica gigas, against scopolamine-or AÃŸ(1-42)-induced cognitive dysfunction in mice. J Ethnopharmacol 2012;143(2):611-20.
Li L, Du JK, Zou LY, Wu T, Lee YW, Kim YH. Decursin Isolated from Angelica gigas Nakai rescues PC12 cells from amyloid ÃŸ-protein-induced neurotoxicity through Nrf2-mediated upregulation of heme oxygenase-1: Potential roles of MAPK. Evid Based Complement Alternat Med 2013;2013:467245.
Cho JH, Kwon JE, Cho Y, Kim I, Kang SC. Anti-inflammatory effect of Angelica gigas via heme oxygenase (HO)-1 expression. Nutrients 2015;7(6):4862-74.
Ma YF, Jung JY, Jung YJ, Choi JH, Jeong WS, Song YS, et al. Anti-inflammatory activities of coumarins isolated from Angelica gigas Nakai on LPS-stimulated RAW 2647 Cells. J Food Sci Nutr 2009;14:179-87.
Kang SY, Kim YC. Neuroprotective coumarins from the root of Angelica gigas: Structure-activity relationships. Arch Pharm Res 2007;30(11):1368-73.
Bhatti R, Singh K, Ishar MP, Singh J. The effect of Allium sativum on ischemic preconditioning and ischemia reperfusion induced cardiac injury. Indian J Pharmacol 2008;40(6):261-5.
King J. A routine method for the estimation of lactic dehydrogenase activity. J Med Lab Technol 1959;16:265-72.
Ochei J, Kolhatkar A. Medical Laboratory Science-Theory and Practice. India: McGraw Hills; 2006. p. 168-70.
Lee S, Lee YS, Jung SH, Shin KH, Kim BK, Kang SS. Antioxidant activities of the decursinol angelate and decursin from Angelica gigas roots. Nat Prod Sci 2003;9(7):170-3.
Somashekar B, Rai SS, Gowda KP. Cardioprotective effects of ethanolic leaf extract of Ipomoea batatas on doxorubicin induced cardiotoxicity in rats. Asian J Pharm Clin Res 2015;8(2):444-50
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