EVALUATION OF BIOACTIVITIES OF MORINDA TINCTORIA LEAVES EXTRACT FOR PHARMACOLOGICAL APPLICATIONS.

Authors

  • Thangavel Sivakumar Department of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, Tamil Nadu, India.
  • Bhagavathi Sundaram Sivamaruthi nnovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
  • Kamaraj Lakshmi Priya Department of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, Tamil Nadu, India.
  • Periyanaina Kesika Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand
  • Chaiyavat Chaiyasut Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.21583

Keywords:

Antimicrobial, Antioxidant, Anti-inflammation, Aqueous extraction, Morinda tinctoria

Abstract

 Objective: The present study was aimed to prepare Morinda tinctoria leaves extracts with the different solvent system and to evaluate the bioactivities.

Methods: The extracts of M. tinctoria were qualitatively analyzed for the primary phytochemical content. The functional groups of extract were determined by Fourier-transform infrared spectroscopy (FT-IR) analysis. The antimicrobial properties were determined by plate assays. The antioxidant and in vitro anti-inflammatory properties and membrane stabilizing nature of aqueous extract of M. tinctoria (AEM) were measured using a spectrophotometer.

Results: The aqueous, ethanolic, and acetone extracts of M. tinctoria were prepared. AEM contains quinones, steroids, terpenoids, phenols, glycosides, and tannins. FTIR result showed that AEM comprises of alkyl halides, 1°, 2° amines, aromatics, aliphatic amines, alcohols, carboxylic acids, esters, ethers, and alkanes, saturated aliphatic, and phenolic groups. The antimicrobial property of M. tinctoria varied based on the solvent used for the extraction. About 86.90±0.36, 78.58±0.13, and 80.33±0.09% of total antioxidant capacity, reducing power, and hydrogen peroxide scavenging activity were observed in AEM, respectively. The 1, 1- diphenyl 2-picrylhyorazyl and 2, 2-Azinobis-(3 ethylbenzothiazoline-6-sulfonic acids) assay results indicated 85.20±0.50 and 52.41±0.60% of free radical scavenging activity in AEM. The protease activity (44.10±0.26%) and protein degradation (44.38±0.58%) were proscribed by AEM. AEM prevents 69.36±0.20% of cell lysis.

Conclusion: The results revealed that the AEM leaves were harmless and enriched with potent bioactive principles, which is further used for food and pharmacological applications.

References

Newman DJ, Cragg GM, Snader KM. The influence of natural products upon drug discovery. Nat Prod Rep 2000;17:215-34.

Wang MY, West BJ, Jensen CJ, Nowicki D, Su C, Palu AK, et al. Morinda citrifolia (Noni): A literature review and recent advances in noni research. Acta Pharmacol Sin 2002;23:1127-41.

Kumaresan PT, Saravanan A. Anticonvulsant activities of Morinda tinctoria Roxb. Afr J Pharm Pharmacol 2009;3:63-5.

Ramasubramanian V, Palanivelu K. Biochemical and histopathological changes on alloxan induced diabetic albino rats treated with leaves of Morinda tinctoria Roxb. Int J Curr Med Pharm Res 2016;2:316-22.

Suneetha M, Ravindhranath K. New bio-sorbents in controlling ammonia pollution in wastewaters. J Chem Pharm Res 2012;4:526-37.

Vijayalakshmi R, Venkatachalam R, Periyathambi T, Saravanan P. Biosorption of reactive red 198 from an aqueous solution using Morinda tinctoria. IJRTSAT 2013;9:36-40.

Vadivu R, Chitra V, Susmitha, Sindhu K, Muthusamy P, Suseela L. The effect of ethanol extract of Morinda tinctoria Roxb leaves on antiulcer activity in rats. NPAIJ 2008;4:99-102.

Sait II, Harindran J, Vahab AA, Jeena JL, Nasli S, John J. Potential hepatoprotective effect and antioxidant role of methanol extract of Morinda tinctoria in carbon tetrachloride induced hepatotoxicity in albino rats. Int J Pharm 2014;4:363-8.

Amerasan D, Murugan K, Panneerselvam C, Kanagaraju N, Kovendan K, Mahesh KP. Bioefficacy of Morinda tinctoria and pongamiaglabra plant extracts against the malaria vector Anopheles stephensi (Diptera: Culicidae). J Entomol Acarol Res 2015;47:31-40.

Kumar SN, Simon KN, Parvathy B. In vitro antimicrobial activities and phytochemical analysis of Morinda tinctoria (L) leaf extracts. Int J Pharm Sci Rev Res 2016;38:58-61.

Ruskin RS, Kumari BV, Chitarasu T. Qualitative phytochemical and FTIR analysis of root extracts of Canthium parviflorum Lam. J Chem Pharm Sci 2014;2:122-7.

Pengkumsri N, Sivamaruthi BS, Sirilun S, Peerajan S, Kesika P, Chaiyasut K, et al. Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice. Food Sci Technol (Campinas) 2017;37:124-30.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of Vitamin E1. Anal Biochem 1999;269(2):337-41.

Shahriar M, Hossain MD, Sharmin AF, Akhter S, Haque MD, Bhuiyan MA. In vitro antioxidant and free radical scavenging activity of Withania somnifera root. IOSR J Pharm 2013;3:38-47.

Peerajan S, Chaiyasut C, Sirilun S, Chaiyasut K, Kesika P, Sivamaruthi BS. Enrichment of nutritional value of Phyllanthus emblica fruit juice using the probiotic bacterium, Lactobacillus paracasei HII01 mediated fermentation. Food Sci Technol (Campinas) 2016;36:116-23.

Pengkumsri N, Chaiyasut C, Saenjum C, Sirilun S, Peerajan S, Suwannalert P, et al. Physicochemical and antioxidative properties of black, brown and red rice varieties of northern Thailand. Food Sci Technol (Campinas) 2015;35:331-8.

Chaiyasut C, Sivamaruthi BS, Pengkumsri N, Sirilun S, Peerajan S, Chaiyasut K, et al. Anthocyanin profile and its antioxidant activity of widely used fruits, vegetables, and flowers in Thailand. Asian J Pharm Clin Res 2016;9:218-24.

Reshma, Arun KP, Brindha P. In vitro anti-inflammatory, antioxidant and nephroprotective studies on leaves of Aegle marmelos and Ocimum sanctum. Asian J Pharm Clin Res 2014;7:121-9.

Rajalakshmi GR, Jyoti H. Anti-Inflammatory activity of Wrightia tinctoria leaves by membrane stabilization. Int J Pharm Sci Res 2012;3:497-9.

Kolli D, Amperayani KR, Parimi U. Total phenolic content and antioxidant activity of Morinda tinctoria leaves. Indian J Pharm Sci 2015;77:226-30.

Sreena KP, Poongothai A, Soundariya SV, Srirekha G, Santhi R, Annapoorani S. Evaluation of in vitro free radical scavenging efficacy of different organic extracts of Morinda tinctoria leaves. Int J Pharm Pharm Sci 2011;3 Suppl 3:207-9.

Shanthi G, Saridha D, Mariappan V. Pharmacognostical studies on Morinda tinctoria. Roxb. Int J Pharm Pharm Sci 2012;4:636-8.

Arunachalam KD, Kuruva JK, Hari S, Annamalai SK, Baskaran KV. HPTLC finger print analysis and phytochemical investigation of Morinda tinctoria Roxb leaf extracts by HPLC and GS MS. Int J Pharm Pharm Sci 2015;7:360-6.

Jayasinghe UL, Jayasooriya CP, Bandara BM, Ekanayake SP, Merlini L, Assante G, et al. Antimicrobial activity of some Sri Lankan Rubiaceae and Meliaceae. Fitoterapia 2002;73:424-7.

Kaniakumari D, Selvakumar P, Loganathan V. In vitro antibacterial and antifungal activities of Morinda tinctoria leaf in different solvents. Pharmacia 2013;2:52-8.

Sivaraman D, Muralidharan P. Cytoprotective effect of Morinda tinctoria Roxb. Against surgical and chemical factor induced gastric and duodenal ulcers in rats. Ulcers 2011;2011:1-9.

Surendiran G, Mathivanan N. Hepatoprotective properties of Morinda pubescens J.E. Smith (Morinda tinctoria Roxb.) fruit extract. Med Chem Res 2011;20:307-13

Published

2018-02-01

How to Cite

Sivakumar, T., B. S. Sivamaruthi, K. Lakshmi Priya, P. Kesika, and C. Chaiyasut. “EVALUATION OF BIOACTIVITIES OF MORINDA TINCTORIA LEAVES EXTRACT FOR PHARMACOLOGICAL APPLICATIONS.”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 2, Feb. 2018, pp. 100-5, doi:10.22159/ajpcr.2018.v11i2.21583.

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