THE EFFECT OF LUNASIN FROM INDONESIAN SOYBEAN EXTRACT ON INDUCIBLE NITRIC OXIDE SYNTHASE AND β-CATENIN EXPRESSION IN DEXTRAN SODIUM SULFATE-INDUCED MICE COLON

  • Kusmardi Kusmardi Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Indonesia
  • Nessa Nessa Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Indonesia.
  • Ari Estuningtyas Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Indonesia.
  • Aryo Tedjo Department of Chemical Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia.
  • Puspita Eka Wuyung Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Indonesia

Abstract

 

 Objective: Inflammatory bowel diseases are idiopathic inflammatory disorders of the gastrointestinal tract. Chronic inflammation that occurs in the colon can develop into colon cancer. Lunasin has been known to resist inflammatory reactions induced by lipopolysaccharide in vitro. The role of lunasin as anti-inflammatory in vivo is not widely known. In this study, we analyzed the effect of lunasin from Indonesian soybean to decrease the risk of inflammation by analyzing the expressions of inducible nitric oxide synthase (iNOS) and β-catenin.

Methods: A total of 30 mice are divided into six groups. Normal group was not induced by dextran sodium sulfate (DSS). The other groups were induced by DSS 2% through drinking water for 9 days. After 9 days, negative group did not receive any treatment, besides the other groups received treatment given lunasin dose 20 mg/kg BB and 40 mg/kg BB, commercial lunasin, and positive given aspirin. Treatment was performed for 5 weeks. Immunohistochemistry scores of iNOS and β-catenin proteins were analyzed using statistical tests.

Results: Expression of iNOs decreased significantly (p<0.05) with an average score of 60.134 and 35.473. Expression of β-catenin decreased significantly (p<0.05) with an average score of 15.58 and 11.131.

Conclusion: Lunasin dose 20 mg/kg BW and 40 mg/kg BB were able to inhibit inflammation by decreasing the expressions of iNOS, and β-catenin.

 

Keywords: inflammation, lunasin, iNOs, β-catenin

Author Biographies

Kusmardi Kusmardi, Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Indonesia
Department of Anatomical Pathology
Nessa Nessa, Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Indonesia.
Department of Pharmacology and Therapeutic
Ari Estuningtyas, Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Indonesia.
Department of Pharmacology and Therapeutic
Aryo Tedjo, Department of Chemical Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia.
Department of Chemical Medicine
Puspita Eka Wuyung, Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Indonesia
Department of Anatomical Pathology

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Kusmardi, K., N. Nessa, A. Estuningtyas, A. Tedjo, and P. E. Wuyung. “THE EFFECT OF LUNASIN FROM INDONESIAN SOYBEAN EXTRACT ON INDUCIBLE NITRIC OXIDE SYNTHASE AND β-CATENIN EXPRESSION IN DEXTRAN SODIUM SULFATE-INDUCED MICE COLON”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 1, Jan. 2018, pp. 416-20, doi:10.22159/ajpcr.2018.v11i1.21811.
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