• Wachid Putranto Student of Doctoral Programme, Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia.
  • Imam Effendi Department of Internal Medicine,Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia.
  • Kusmardi Kusmardi Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • Hamzah Shatri Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.



Angiotensin converting enzyme inhibitor, Calcium channel blocker, Nil, Peritoneal fibrosis


 Objective: The objective of this research was to evaluate the effect of combination between lisinopril and diltiazem in reducing expression of transforming growth factor-β (TGF-β) and fibrosis peritoneum in a male rat treated with continuous ambulatory peritoneal dialysis (CAPD).

Method: This was an experimental study, post-test only control group design. Thirsty Dawley Sprague rats are divided into five groups: Control group (Group 1), CAPD liquid 4,25% (Group 2), lisinopril 1.44 mg oral and CAPD (Group 3), and diltiazem CD 6.48 mg oral and CAPD (Group 4), lisinopril 1.44 mg+diltiazem CD 6.48 mg oral and CAPD (Group 5). After 4 weeks, rats sacrificed expression of TGF-β and peritoneal fibrosis are conducted by histopathology with hematoxylin-eosin staining and immunology with anti-human-TGF-β.

Result: Twenty peritoneal of rats can be examined. Mean score of TGF-β in control group is 1, 8, in CAPD group is 2, in lisinopril and CAPD group is 1, 8, in diltiazem CD and CAPD group is 1, 8, in lisinopril and diltiazem CD and CAPD group is 1, 7 (p=0.959). Mean score of peritoneal fibrosis in control group is 1, 1, in CAPD group is 2, 6, in lisinopril and CAPD group is 1, 2, in diltiazem CD and CAPD group is 1, 3, in lisinopril and diltiazem CD, and CAPD group is 1, 5 (p=0,268).

Conclusion: Combination of lisinopril and diltiazem lower the expression of TGF-β and peritoneum fibrosis better than lisinopril or diltiazem but statistically not significant. Combination of lisinopril and diltiazem lower the peritoneal fibrosis but statistically not significant, and it does not better than lisinopril or diltiazem.


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Author Biographies

Wachid Putranto, Student of Doctoral Programme, Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia.

Internal Medicine

Imam Effendi, Department of Internal Medicine,Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia.

Anatomical pathology

Kusmardi Kusmardi, Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Internal Medicine

Hamzah Shatri, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Internal medicine


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How to Cite

Putranto, W., I. Effendi, K. Kusmardi, and H. Shatri. “COMBINATION EFFECT OF LISINOPRIL AND DILTIAZEM AS ANTI FIBROSIS IN PERITONEUM OF RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 3, Mar. 2018, pp. 71-76, doi:10.22159/ajpcr.2018.v11i3.23152.



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