COMBINATION OF SAMBILOTO (ANDROGRAPHIS PANICULATA (BURM.F.) NEES) EXTRACT AND SPIRULINA (ARTHROSPIRA PLATENSIS GOMONT) TO PREVENT ANEMIA IN MICE INFECTED WITH PLASMODIUM BERGHEI ANKA


Kusmardi Kusmardi, Berna Elya, Tri Wahyuni, Rafika Indah Paramita

Abstract


Objective: Anemia in acute malaria occurs because there is massive lysis of erythrocytes as a result of an increase in parasitemia or use of drugs that may induce hemolysis. In this research, it was tested the effect of administration of a combination of sambiloto (Andrographis paniculata Nees) extract and spirulina (Arthrospira platensis Gomont). This combination is aimed to obtain antiparasite activity of sambiloto and increasing of hemoglobin (Hb) from spirulina.

Methods: This research was conducted with a completely randomized design using 75 mice of Swiss Webster strain. Test groups were consisted of AP, AP+ES, AP+PS, carboxymethyl cellulose, and dihydroartemisinin-piperaquine. All mice were infected with Plasmodium berghei on the day 0. The test material extracts were given 3 days before infection (H-3) and every day for 28 days after infection. Parasitemia data were taken on day 3, 7, 10, 15, 21, and 28, while the data for erythrocyte count and Hb level were taken on day 3, 10, and 21. Data processing was conducted using one-way ANOVA, followed by post hoc test.

Results: This study showed that combination of sambiloto extract and spirulina powder (AP+PS) gave a significant difference result in suppressing the percentage of parasitemia (p=0.02), increasing the erythrocyte count (p=0.03), and Hb level (p=0.01) at the 15th day, compared to the group given only sambiloto (AP).

Conclusion: Combination of spirulina powder and sambiloto extract will decrease parasitemia and increase the number of erythrocyte and Hb in mice that infected by P. berghei.

 

 


Keywords


Antimalaria, Andrographis paniculata Nees, Arthrospira platensis Gomont, Hematology.

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References


Departement Kesehatan RI. Agency for Health Research and Development, Laporan Riset Kesehatan Dasar (RISKESDAS). Jakarta: Departement Kesehatan RI; 2013.

Sitorus H, Salim M, Lasbudi PA. The pattern of traditional medicinal plants in Segara Kembang Village and Tungku Jaya village District of Ogan Komering Ulu. J Pembang Manus 2011;5(1):1-12.

Verma AK, Prasad SB, Rongpi T, Arjun J. Traditional healing with animals (zoo-therapy) by the major ethnic group of Karbi Anglong district of Assam, India. Int J Pharm Pharm Sci 2014;6(8):593-600.

Gohain N, Prakash A, Gogoi K, Bhattacharya DR, Nilanju P, Dahutia C, et al. An ethnobotanical survey of anti-malarial plants in some highly malaria affected districts of Assam. Int J Pharm Pharm Sci 2015;7(9):147-52.

Zein U. Perbandingan Efikasi Ekstrak Herba Sambiloto (Andrographis paniculata Nees) Tunggal Dan Kombinasi Masing-Masing Dengan Artesunat Dan Klorokuin Pada Pasien Malaria Falsiparum Tanpa Komplikasi, Medan, Disertasi, Sekolah Pasca Sarjana, Universitas Sumatera Utara; 2009.

Zein U, Fitri LE, Saragih A. Comparative study of antimalarial effect of sambiloto (Andrographis paniculata) extract, chloroquine and artemisinin and their combination against Plasmodium falciparum in-vitro. Acta Med Indones 2013;45(1):38-43.

Kisdjamiatun S, Kartikawati H, Wibisono A. Effect of Andrographis paniculata herbal extract (sambiloto) on anemia and survival of Balb / c mice during infection of Plasmodium berghei Anka. Med Med Indones 2009;39(3):34-43.

Iribhogbe OI, Agbaje EO, Oreagba IA, Aina OO, Ota AD. Antioxidant based combination therapy in malaria: In vivo study in Plasmodium berghei infected mice. Biolife 2013;1(2):70-7.

Dewi RS. Spirulina platensis Mencegah Penurunan Komponen Darah Perifer Pada Tikus (Rattus norvegicus) Yang Diberikan Cyclophosphamide, Tesis, Program Pasca Sarjana Biomedik, Universitas Udayana; 2014.

Chaiklahan R, Chirasuwan N, Loha V, Bunnag B. Lipid and fatty acid extraction from Cyanobacterium spirulina. Sci Asia 2008;34:299-305.

Moll K, Ljungström I, Perlmann H, Acherf A, Wahlgren M. Methods in Malaria Research, American Type Culture Collection. Manassas: University Boulevard; 2008.

Kusumawardhani D, Widyawaruyanti A, Kusumawati I. The antimalarial effect of standardized sambiloto extract (parameter of andrografolida content) in mice infected with P. berghei. Maj. Farmasi Airlangga 2005;5(1):25-9.

Gaur P. Evaluation of safety aspect of Andrographis paniculata in Swiss-Albino mice at sub-acute level. Int Ayurvedic Med J 2014;2(5):681-6.

Murtini JT, Triwibowo R, Indriati N, Ariyani F. Uji toksisitas sub kronik Spirulina platensis secara in-vivo. J Pascapanen Bioteknol Kelaut Perikan 2010;5(2):123-33.

Tripathy S, Chakraborty SP, Roy S. Superoxide radical generation mediated Plasmodium berghei infection in Swiss mice. Al Ameen J Med Sci 2012;5(1):69-81.

Tjahjani S, Khiong K. Potential red fruit as an antioxidant against malaria berghei in Balb / C strain mice. Maj Kedokt Indones 2010;60(12):571-5.

Edagha IA, Atting IA, Bassey RB, Bassey EI, Ukpe SJ. Erythropoietic and hepatoprotective potential of ethanolic extract of Nuclea latifolia in mice infected with Plasmodium berghei. Am J Med Sci 2014;2(1):7-12.

Bukoye O, Musbau A. Immune modulation potentials of aqueous extract of Andrographis paniculata leaves in rat. Researcher 2013;3(1):48-57.

Percário S, Moreira DR, Gomes BA, Ferreira ME, Gonçalves AC, Laurindo PS, et al. Oxidative stress in malaria. Int J Mol Sci 2012;13(2):16346-72.

Elmalawany AM, Salem TA, Mohamed AH, Osman GY. Effect of blue green algae on some biochemical and hematological markers in mice. Int J Adv Res 2014;2(2):568-74.

Murray RK, Granner DK, Rodwell VW. Biokimia Harper. 27nd ed. Jakarta: Penerbit Buku Kedokteran EGC; 2009.

Harijanto PN. Malaria, Dari Molekular ke Klinis. Jakarta: Penerbit Buku Kedokteran EGC; 2009.




About this article

Title

COMBINATION OF SAMBILOTO (ANDROGRAPHIS PANICULATA (BURM.F.) NEES) EXTRACT AND SPIRULINA (ARTHROSPIRA PLATENSIS GOMONT) TO PREVENT ANEMIA IN MICE INFECTED WITH PLASMODIUM BERGHEI ANKA

Keywords

Antimalaria, Andrographis paniculata Nees, Arthrospira platensis Gomont, Hematology.

DOI

10.22159/ajpcr.2017.v10i12.21231

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 269-273

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

14 Views | 34 Downloads

Authors & Affiliations

Kusmardi Kusmardi
Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Indonesia

Berna Elya
Department of Phytochemistry and Pharmacognosy, Laboratory of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Tri Wahyuni
National Institute of Health Research and Development, Ministry of Health of Indonesia, Jakarta, Indonesia.
Indonesia

Rafika Indah Paramita
Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Indonesia


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