ALPHA-GLUCOSIDASE INHIBITING ACTIVITY FROM THE MOST ACTIVE FRACTION OF CALOPHYLLUM TOMENTOSUM, WIGHT LEAVES


Berna Elya, Nuraini Puspitasari, Fasya Hadaina Maharani

Abstract


Objective: The purpose of this study was to obtain the inhibitory concentration, 50% value from the most active fraction of methanolic extract of leaf Calophyllum tomentosum, Wight, and to identify the group of compounds contained in the most active fraction.

Methods: The methanol extract was fractionated using the silica gel static phase, the n-hexane motion phase, ethyl acetate, and methanol enhanced by its polarity.

Results: There were nine selected fractions tested in inhibitory activity using an enzyme activity inhibitory assay.

Conclusions: The results showed the inhibition by the most active fraction is 51.288 μg/mL; this fraction contains of flavonoid, saponin, and terpenoid.


Keywords


Diabetes mellitus, Calophyllum tomentosum Wight., Fractionation, α-glucosidase, Enzyme inhibitory assay.

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About this article

Title

ALPHA-GLUCOSIDASE INHIBITING ACTIVITY FROM THE MOST ACTIVE FRACTION OF CALOPHYLLUM TOMENTOSUM, WIGHT LEAVES

Keywords

Diabetes mellitus, Calophyllum tomentosum Wight., Fractionation, α-glucosidase, Enzyme inhibitory assay.

DOI

10.22159/ajpcr.2017.v10s5.23101

Date

01-10-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Special Issue October 2017 [PTMDS 2017] Page: 73-75

Print ISSN

0974-2441

Online ISSN

2455-3891

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Authors & Affiliations

Berna Elya
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Nuraini Puspitasari
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Fasya Hadaina Maharani
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia


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