Abdul Mun'im, Muhammad Ashar Munadhil, Nuraini Puspitasari, Azminah ., Arry Yanuar


Objectives: To evaluate the angiotensin converting enzyme (ACE) inhibitory activity of melinjo (Gnetum gnemon) seed extract and to study molecular
docking of stilbene contained in melinjo seeds.
Methods: Melinjo seed powders were extracted with n-hexane, dichloromethane, ethyl acetate, methanol, and water successively. The extracts were
evaluated ACE inhibitory activities using ACE kit-Wist and the phenolic content using Folin–Ciocalteu method. The extract demonstrated the highest
ACE inhibitory activity was subjected to liquid chromatography-mass spectrometry (LC-MS) to know its stilbene constituent. The stilbene constituents
in melinjo seed were performed molecular docking using AutoDock Vina, and ligand-receptor Interactions were processed using Ligand Scout.
Results: The ethyl acetate extract demonstrated the highest ACE inhibition activity with inhibitory concentration 50% value of 9.77 × 10−8 μg/mL
and the highest total phenolic content (575.9 mg gallic acid equivalent/g). Ultra-performance LC-MS analysis of ethyl acetate extract has detected the
existency of resveratrol, gnetin C, ε-viniferin, and gnemonoside A/B. These compounds displayed similar physiochemical properties to lisinopril (ACE
inhibitor), as in silico molecular docking studies demonstrated that they fit into the lisinopril receptors.
Conclusion: In vitro analysis ethyl acetate extract from melinjo seeds demonstrated the highest ACE inhibitory activity. Molecular docking analysis
indicated that resveratrol dimers, gnetin C and gnemonoside A can be considered ACE inhibitor.
Keywords: Angiotensin converting enzyme inhibitor, Gnetum gnemon, Melinjo, Total phenolic, Antihypertension, Molecular docking.

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Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 243-148

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Authors & Affiliations

Abdul Mun'im
Universitas Indonesia

Muhammad Ashar Munadhil

Nuraini Puspitasari

Azminah .

Arry Yanuar

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