ANTIOXIDANT ACTIVITY AND INHIBITION OF LIPOXYGENASE ACTIVITY ETHANOL EXTRACT OF ENDOSPERM ARENGA PINNATA (WURMB) MERR.

Berna Elya; Nuraini Puspitasari; Annisa Chairani Sudarmin

doi:10.22159/ajpcr.2017.v10s5.23102

Authors

Berna Elya Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Nuraini Puspitasari Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Annisa Chairani Sudarmin Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23102

Keywords:

Arenga pinnata, Sugar palm fruit, 2, 2-Diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Lipoxygenase

Abstract

Objective: The purpose of this study is to determine the antioxidant activity and the potential inhibition of lipoxygenase activity from sugar palm fruit.

Methods: Antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Inhibition of lipoxygenase activity was performed in vitro. Sugar palm fruit was macerated with 95% ethanol.

Results: The results showed that sugar palm fruit ethanol extract has antioxidant activity when using DPPH method with EC50 of 141.3929 Î¼g/mL and the FRAP method with EC50 of 60.2083 Î¼g/mL. Inhibition test of lipoxygenase activity showed inhibitory concentration 50% value of 71.376 Î¼g/mL.

Conclusions: Ethanol extract of endosperm Arenga pinnata has antioxidant activity, as determined using the DPPH method with EC50of 141.3929 Î¼g/mL and the FRAP method with EC50 of 60.2083 Î¼g/mL.

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