PROFILE OF ANTIBACTERIAL ACTIVITY OF FRACTIONS FROM METHANOL EXTRACTS OF GARCINIA LATISSIMA MIQ. FRUIT RIND


NENENGSITI SILFI AMBARWATI

Abstract


Objectives: A previous study showed that methanol extracts of Garcinia latissima Miq. demonstrated antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. The aim of this study was to obtain active antibacterial fractions from methanol extracts of G. latissima Miq.

Methods: Fractionation of extracts was performed on G60 silica gel column chromatography using n-hexane eluent, ethyl acetate, and methanol. Antibacterial tests were done using the paper disc method to determine the zone of inhibition, the microdilution method to determine the minimuminhibitory concentration (MIC), and a bioautographic test.

Results: Fractions A-E, and F had zones of inhibition against B. subtilis. Fractions A-E had zones of inhibition against S. aureus. Fractions C-E had zones of inhibition against P. aeruginosa. The bio-autograph test showed zones of inhibition on several bio-autographic spots, indicating that activecompounds were obtained from the fractionation of methanol extract from G. latissima Miq. fruit rind. Fraction D’s MIC against B. subtilis, S. aureus, and P. aeruginosa was 312.5 ppm. The MIC of Fraction C against B. subtilis and of Fraction R against P. aeruginosa also was 312.5 ppm.

Conclusion: Fraction D was the most active fraction against the three test bacteria.


| PDF |


About this article

Title

PROFILE OF ANTIBACTERIAL ACTIVITY OF FRACTIONS FROM METHANOL EXTRACTS OF GARCINIA LATISSIMA MIQ. FRUIT RIND

Date

01-10-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Special Issue October 2017 [PTMDS 2017] Page: 66-68

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

0 Views | 0 Downloads

Authors & Affiliations

NENENGSITI SILFI AMBARWATI
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.