PROFILE OF ANTIBACTERIAL ACTIVITY OF FRACTIONS FROM METHANOL EXTRACTS OF GARCINIA LATISSIMA MIQ. FRUIT RIND


Nenengsiti Silfi Ambarwati, Amarila Malik, Berna Elya, Muhammad Hanafi

Abstract


Objectives: A previous study showed that methanol extracts of Garcinia latissima Miq. demonstrated antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. The aim of this study was to obtain active antibacterial fractions from methanol extracts of G. latissima Miq.

Methods: Fractionation of extracts was performed on G60 silica gel column chromatography using n-hexane eluent, ethyl acetate, and methanol. Antibacterial tests were done using the paper disc method to determine the zone of inhibition, the microdilution method to determine the minimum inhibitory concentration (MIC), and a bioautographic test.

Results: Fractions A-E, and F had zones of inhibition against B. subtilis. Fractions A-E had zones of inhibition against S. aureus. Fractions C-E had zones of inhibition against P. aeruginosa. The bio-autograph test showed zones of inhibition on several bio-autographic spots, indicating that active compounds were obtained from the fractionation of methanol extract from G. latissima Miq. fruit rind. Fraction D’s MIC against B. subtilis, S. aureus, and P. aeruginosa was 312.5 ppm. The MIC of Fraction C against B. subtilis and of Fraction R against P. aeruginosa also was 312.5 ppm.

Conclusion: Fraction D was the most active fraction against the three test bacteria.


Keywords


Garcinia latissima Miq., Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Zone of inhibition, Minimum inhibitory concentration, Bio-autograph.

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References


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About this article

Title

PROFILE OF ANTIBACTERIAL ACTIVITY OF FRACTIONS FROM METHANOL EXTRACTS OF GARCINIA LATISSIMA MIQ. FRUIT RIND

Keywords

Garcinia latissima Miq., Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Zone of inhibition, Minimum inhibitory concentration, Bio-autograph.

DOI

10.22159/ajpcr.2017.v10s5.23099

Date

01-10-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol. 10, Special Issue (Oct.), 2017 [PTMDS 2017] Page: 66-68

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

94 Views | 27 Downloads

Authors & Affiliations

Nenengsiti Silfi Ambarwati
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Amarila Malik
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Berna Elya
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
Indonesia

Muhammad Hanafi
Research Centre for Chemistry, Indonesian Institute of Sciences (LIPI), Tangerang, Indonesia.
Indonesia


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