ANTIOXIDANT ACTIVITY AND LIPOXYGENASE INHIBITORY ASSAY WITH TOTAL FLAVONOID CONTENT OF GARCINIA LATERIFLORA BLUME LEAVES EXTRACT


SISMITA AVELIA

Abstract


Objective: Garcinia lateriflora Blume has been reported to have antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method with methanol,
ethyl acetate, and n-hexane leaves extract inhibitory concentration 50% (IC
) levels of 6.18, 8.03, and 156.8 µg/mL, respectively. Meanwhile, there has
been no literature regarding G. lateriflora Blume’s lipoxygenase inhibition activity. The aim of this study was to determine the potential antioxidant
activity and lipoxygenase inhibition activity of three leaf extracts of G. lateriflora Blume.
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Methods: These study test methods involved an assessment of antioxidant activity using the ferric reducing antioxidant power method, an assessment
of lipoxygenase inhibition activity through the in vitro method, and a qualitative analysis of flavonoid and total flavonoid content using thin-layer
chromatography and the AlCl
3
colorimetric method to reveal the most active extract.
Results: Based on the assessment for methanol, ethyl acetate, and n-hexane, the results showed that the effective concentration 50% levels of the
antioxidant activity of G. lateriflora Blume leaves extract were 9.567, 16.555, and 50.550 µg/mL, respectively. Furthermore, the IC
levels of the
lipoxygenase inhibition activity were 0.693, 0.793, and 1.316 µg/mL, respectively. The most active extract for both of the tests was methanol extract,
which has a total flavonoid content of 6.298 mg quercetin equivalents/g.
Conclusions: Based on the test results, it can be concluded that G. lateriflora Blume leaves extracts exhibit antioxidant and lipoxygenase inhibition
activities, with methanol extract as the most active extract, containing more flavonoid than the other two extracts.
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About this article

Title

ANTIOXIDANT ACTIVITY AND LIPOXYGENASE INHIBITORY ASSAY WITH TOTAL FLAVONOID CONTENT OF GARCINIA LATERIFLORA BLUME LEAVES EXTRACT

Date

01-10-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Special Issue October 2017 [PTMDS 2017] Page: 163-165

Print ISSN

0974-2441

Online ISSN

2455-3891

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Authors & Affiliations

SISMITA AVELIA
Indonesia


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