ANTIOXIDANT ACTIVITY AND LIPOXYGENASE INHIBITORY ASSAY WITH TOTAL FLAVONOID CONTENT OF GARCINIA LATERIFLORA BLUME LEAVES EXTRACT


Sismita Avelia, Marista Gilang Mauldina, Berna Elya

Abstract


 

Objective: Garcinia lateriflora Blume has been reported to have antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method with methanol, ethyl acetate, and n-hexane leaves extract inhibitory concentration 50% (IC50) levels of 6.18, 8.03, and 156.8 μg/mL, respectively. Meanwhile, there has been no literature regarding G. lateriflora Blume’s lipoxygenase inhibition activity. The aim of this study was to determine the potential antioxidant activity and lipoxygenase inhibition activity of three leaf extracts of G. lateriflora Blume.

Methods: These study test methods involved an assessment of antioxidant activity using the ferric reducing antioxidant power method, an assessment of lipoxygenase inhibition activity through the in vitro method, and a qualitative analysis of flavonoid and total flavonoid content using thin-layer chromatography and the AlCl3 colorimetric method to reveal the most active extract.

Results: Based on the assessment for methanol, ethyl acetate, and n-hexane, the results showed that the effective concentration 50% levels of the antioxidant activity of G. lateriflora Blume leaves extract were 9.567, 16.555, and 50.550 μg/mL, respectively. Furthermore, the IC50 levels of the lipoxygenase inhibition activity were 0.693, 0.793, and 1.316 μg/mL, respectively. The most active extract for both of the tests was methanol extract, which has a total flavonoid content of 6.298 mg quercetin equivalents/g.

Conclusions: Based on the test results, it can be concluded that G. lateriflora Blume leaves extracts exhibit antioxidant and lipoxygenase inhibition activities, with methanol extract as the most active extract, containing more flavonoid than the other two extracts.


Keywords


Antioxidant, Ferric reducing antioxidant power, Garcinia lateriflora Blume, Flavonoid, Lipoxygenase.

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About this article

Title

ANTIOXIDANT ACTIVITY AND LIPOXYGENASE INHIBITORY ASSAY WITH TOTAL FLAVONOID CONTENT OF GARCINIA LATERIFLORA BLUME LEAVES EXTRACT

Keywords

Antioxidant, Ferric reducing antioxidant power, Garcinia lateriflora Blume, Flavonoid, Lipoxygenase.

DOI

10.22159/ajpcr.2017.v10s5.23122

Date

01-10-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol. 10, Special Issue (Oct.), 2017 [PTMDS 2017] Page: 163-165

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

71 Views | 41 Downloads

Authors & Affiliations

Sismita Avelia
Department of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
Indonesia

Marista Gilang Mauldina
Department of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
Indonesia

Berna Elya
Department of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
Indonesia


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