PRELIMINARY MYCOCHEMICAL, GAS CHROMATOGRAPHY–MASS SPECTROSCOPY ANALYSIS, AND ANTIMICROBIAL PROPERTIES OF CALOCERA VISCOSA (PERS.) FR.

  • NAVEEN KUMAR NAIK S Department of P.G Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Shimoga, Karnataka – 577451, India.
  • ASHWATHANARAYANA R Department of P.G Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Shimoga, Karnataka – 577451, India.
  • RAJA NAIKA Department of P.G Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Shimoga, Karnataka – 577451, India.

Abstract

Objectives: Calocera viscosa, commonly called as the yellow stagshorn, is a jelly fungus, belongs to the family of Dacrymycetales, unknown for its medicinal properties and biological activities.


Method: The sporocarps of C. viscosa (Pers.) Fr. were collected from Agumbe, Karnataka. Mycochemical and Gas chromatography–mass spectroscopy (GC–MS) analysis done by standard procedures and antibacterial activity was done by agar well diffusion method.


Results: Physicochemical was analyzed and results revealed the highest percentage of alcohol-soluble extractives were present followed by ash content. Alcohol-soluble extractives were 20.76%, total moisture content (10.9%), and foreign matter (0.5%). Extraction was done by Soxhlet apparatus using petroleum ether, chloroform, and ethanol and subjected to qualitative mycochemicals analysis both petroleum ether and chloroform extract confirms less mycochemicals, whereas ethanolic extract revealed the presence of alkaloids, tannins, flavonoids, sterols, glycosides, terpenoids, and phenols. GC–MS analysis of ethanoic extract showed many known bioactive compounds in that, 19 compounds were unknown and 21 compounds were known for its medicinal properties, most of them were food additives and flavoring agents. Antibacterial potentials were studied against pathogenic bacteria revealed that ethanolic extract showed appreciable zone of inhibition against pathogenic bacteria, in that maximum zone of inhibition showed against Klebsiella pneumonia followed by Escherichia coli and Staphylococcus aureus.


Conclusion: C. viscosa (Pers.) Fr. sporocarp can be explored for potential antibacterial with rich full of useful mycochemicals.

Keywords: Calocera viscosa (Pers.) Fr., Preliminary mycochemical analysis, Gas chromatography–mass spectroscopy analysis, Antimicrobial activity, Agumbe.

Author Biography

NAVEEN KUMAR NAIK S, Department of P.G Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Shimoga, Karnataka – 577451, India.

Dept of. Applied Botany, Kuvempu University, Jnana Sahyadri, Shankaraghatta-577451, Shimoga (D), Karnataka

References

1. Pande A. Ascomycetes of Peninsular India. India: Scientific Publisher; 2008.
2. Iqbal D, Raju K, Pawar, Rajeev KR, Sharma S. Physico-chemical standardization of Butea monosperma (Lam) Kuntze (Palasha): An Ayurveda drug. Int J Pharm Qual Assur 2010;2:49-51.
3. Gupta AK. Quality Standards of Indian Medicinal Plants. India: Indian Council of Medicinal Research; 2003.
4. Indrayan AK, Sharma S. Durgapal D. Kumar N, Kumar M. Valued plants from Uttaranchal. Curr Sci 2005;89:1252-5.
5. Ahmad RV, Sharma RK. Proceedings of WHO Training Cum- Workshop, Evaluation of Drug for Standardization. Pharmaceutical lab for Indian Medicine, Ministry of Health and Family Welfare. Ghaziabad: Government of India; 2001.
6. De-Castro MD, Ayuso LE. Soxhlet extraction of solid materials: An outdated technique with a promising innovative future. Anal Chim Acta 1998;369:1-10.
7. Harborne JB. Mycochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. New York: Chapman and Hall Co.; 1998. p. 1-302.
8. Irshad S, Mahmood M, Perveen F. In-vitro anti-bacterial activities of three medicinal plants using agar well diffusion method. Res J Biol 2012;2:1-8.
9. PubChem Glycerin C3H8O3. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/glycerol.
10. PubChem Pyrocatechol C6H6O2. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/catechol.
11. PubChem Coumaran C8H8O. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/2_3-dihydrobenzofuran.
12. PubChem Capric Acid C10H20O2. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/Decanoic_acid.
13. PubChem Lauric Acid C12H24O2. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/lauric_acid.
14. PubChem Vanillic Acid C8H8O4. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/Vanillic_acid.
15. PubChem Myristic acid C14H28O2. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/Tetradecanoic_acid.
16. PubChem Mycol C20H40O. Available from: https://www.pubchem.ncbi. nlm.nih.gov/compound/3_7_11_15-tetramethyl-2-hexadecen-1-ol.
17. PubChem Arachidyl Alcohol C20H42O. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/1-eicosanol.
18. PubChem Oleic Acid C18H34O2. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/oleic_acid.
19. PubChem Methyl Linoleate C19H34O2. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/methyl_linoleate.
20. PubChem Methyl Stearate C19H38O2. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/methyl_stearate.
21. PubChem Octadecanal C18H36O. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/octadecanal.
22. PubChem Octinoxate C18H26O3. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/Octinoxate.
23. PubChem Hexadecanal C16H32O. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/hexadecanal.
24. PubChem 9-Octadecenoic Acid C18H34O2. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/965.
25. PubChem Tetraethylene Glycol Monododecyl Ether C20H42O5. Available from: https://www.pubchem.ncbi.nlm.nih.gov/./Tetraethylene_glycol_ monododecyl_ether.
26. PubChem Ergosterol C28H44O. Available from: https://www.pubchem. ncbi.nlm.nih.gov/compound/ergosterol.
27. PubChem Beta-Sitosterol C29H50O. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/beta-sitosterol.
28. PubChem 16-Hentriacontanone C31H62O. Available from: https://www. pubchem.ncbi.nlm.nih.gov/compound/16-Hentriacontanone.
29. Atri NS. Amino acid profile of a basidiomycetous edible mushroom- Lentinus sajor-caju. Int J Pharm Pharm Sci 2017;9:252-7.
30. Khan F, Chandra R. Effect of physiochemical factors on fruiting body formation in mushroom. Int J Pharm Pharm Sci 2017;9:33-6.
Statistics
9 Views | 23 Downloads
How to Cite
NAIK S, N. K., A. R, and R. NAIKA. “PRELIMINARY MYCOCHEMICAL, GAS CHROMATOGRAPHY–MASS SPECTROSCOPY ANALYSIS, AND ANTIMICROBIAL PROPERTIES OF CALOCERA VISCOSA (PERS.) FR.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 86-93, https://innovareacademics.in/journals/index.php/ajpcr/article/view/27997.
Section
Original Article(s)