INFLUENCE OF PIRDOT LEAF (SAURAUIA VULCANI, KORTH.) EXTRACT ON THE BLOOD GLUCOSE RATE AND HISTOLOGIC DESCRIPTION OF THE RETINA OF MALE MICE (MUS MUSCULUS STRAIN DDW)

  • Adiluddin Hutapea Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan 20155, Indonesia.
  • Salomo Hutahaean Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan 20155, Indonesia.
  • Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan 20155, Indonesia.

Abstract

Objective: This research aims to collect laboratory data on the influence of pirdot leaf extract on the retina and blood glucose rate.

Method: The mice were classified into 5 groups: A control group without any treatment, a control group treated with a single injection of 125 mg/kg body weight (BW) of alloxan as the diabetes mellitus trigger, and three groups treated with intra-muscular alloxan injection to induce diabetes mellitus, and pirdot leaf extract, i.e., 150 mg/kg BW (P1), 200 mg/kg BW (P2), and 250 mg/kg BW (P3) for 8 weeks. Subsequently, the blood glucose rates of the mice were measured, and histologic grazes of their retina layers were prepared using the paraffin method.

Results: The blood glucose rate of the mice treated with 200 mg/kg BW (113.4 mg/dl) pirdot leaf extract significantly differed from that of the mice in the diabetes mellitus control group (364.8 mg/dl) (p<0.05). The ganglion cell layer of the retina increased by up to 7.59 μm, which differed from that of the diabetes mellitus group (3.67 μm) (p<0.05) treated with 250 mg/kg BW pirdot leaf extract. The external plexiform layer increased to 17.88 μm, which differed from that of the diabetes mellitus group (15.71 μm) (p<0.05) treated with 150 mg/kg BW pirdot leaf extract.

Conclusion: The blood glucose rate obtained after treatment with pirdot leaf extract was lower than that of the diabetes mellitus control group.

Keywords: Diabetes mellitus, Diabetic retinopathy.

Author Biographies

Adiluddin Hutapea, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan 20155, Indonesia.
Departement Biology
Salomo Hutahaean, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan 20155, Indonesia.
Departement Biology
Syafruddin Ilyas, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan 20155, Indonesia.
Departement Biology

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Hutapea, A., S. Hutahaean, and S. Ilyas. “INFLUENCE OF PIRDOT LEAF (SAURAUIA VULCANI, KORTH.) EXTRACT ON THE BLOOD GLUCOSE RATE AND HISTOLOGIC DESCRIPTION OF THE RETINA OF MALE MICE (MUS MUSCULUS STRAIN DDW)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 11, Nov. 2018, pp. 389-92, doi:10.22159/ajpcr.2018.v11i11.28316.
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