INFLUENCE OF PIRDOT LEAF (SAURAUIA VULCANI, KORTH.) EXTRACT ON THE BLOOD GLUCOSE RATE AND HISTOLOGIC DESCRIPTION OF THE RETINA OF MALE MICE (MUS MUSCULUS STRAIN DDW)
Objective: This research aims to collect laboratory data on the influence of pirdot leaf extract on the retina and blood glucose rate.
Method: The mice were classified into 5 groups: A control group without any treatment, a control group treated with a single injection of 125 mg/kg body weight (BW) of alloxan as the diabetes mellitus trigger, and three groups treated with intra-muscular alloxan injection to induce diabetes mellitus, and pirdot leaf extract, i.e., 150 mg/kg BW (P1), 200 mg/kg BW (P2), and 250 mg/kg BW (P3) for 8 weeks. Subsequently, the blood glucose rates of the mice were measured, and histologic grazes of their retina layers were prepared using the paraffin method.
Results: The blood glucose rate of the mice treated with 200 mg/kg BW (113.4 mg/dl) pirdot leaf extract significantly differed from that of the mice in the diabetes mellitus control group (364.8 mg/dl) (p<0.05). The ganglion cell layer of the retina increased by up to 7.59 Î¼m, which differed from that of the diabetes mellitus group (3.67 Î¼m) (p<0.05) treated with 250 mg/kg BW pirdot leaf extract. The external plexiform layer increased to 17.88 Î¼m, which differed from that of the diabetes mellitus group (15.71 Î¼m) (p<0.05) treated with 150 mg/kg BW pirdot leaf extract.
Conclusion: The blood glucose rate obtained after treatment with pirdot leaf extract was lower than that of the diabetes mellitus control group.
2. Muha C, Nasee PP. A review article-gestational diabetes mellitus. Inter J Cur Phar Res 2017;9:1.
3. Lai WK, Lo YC. Animal models of diabetic retinopathy: Summary and comparison. J Diab Res 2013;2013:29.
4. Tarr MJ, Kaul K, Chopra M, Kohner ME, Chibber R. Pathophysiology of diabetic retinopathy. ISRN Ophth 2012;2013:343560.
5. Price AS, Wilson ML. Pathophysiology: The Clinical Concept of Disease Processes. 6th ed. Jakarta: EGC; 2005.
6. Sitorus P. Characterization simplisia and ethanolic extract of pirdot (Saurauia vulcani, Korth.) leaves and study of antidiabetic effect in alloxan induced diabetic mice. Int J Chem Tech Res 2015;8:792-3.
7. Sari IM, Ilyas S, Widyawati T, Antika AM. Effect of Lawsonia innermis (Linn) leaves ethanolic extract on blood glucose and malondialdehyde level in alloxan-induced diabetic rats. IOP Conf Ser: E Env Sci 2018;130:4-5.
8. Said AM, Zaki EG, Eldin SA, Nasr M, Azab SS, Elzankavalony AY. Efficacy of intravitreal injection of 2-methoxyestradiol in regression of neovascularization of a retinopathy of prematurity rat model. BMC Opht 2017;17:38.
9. Horstmann L, Schimd H, Heinen PA, Kurschus CF, Dick BH, Joachim CS. Inflammatory demyelination induces glia alterations and ganglion cell loss in the retina of an experimental autoimmune encephalomyelitis model. J Neuro 2013;10:120.
10. Das S, Barman S. Antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of Punica granatum in alloxan-induced non-insulin-dependent diabetes mellitus albino rats. Indian J Pharmacol 2012;44:219-24.
11. Nazaruk J, Borzym-Kluczyk M. The role of triterpenes in the management of diabetes mellitus and its complications. Phytochem Rev 2015;14:675-90.
12. Campbell AN, Reece BJ, Mitchell GL. Biology. Jakarta: Erlangga; 2004. p. 142.
13. Dutta J, Kalitta MC. In vitro hypoglicaemic evaluation of seven culinary plants of North East India against Type 2 diabetes. Asian J Pharm Clin Res 2016;9:212.
14. Fitrianda E, Sukandar YE, Adnyana KE. Antidiabetic activity of extract, fractions, and asiaticoside compound isolated from Centella asiatica Linn. Leaves in alloxan-induced diabetic mice. Asian J Pharm Clin Res 2017;10:269-71.
15. Hutahaean S, Tanjung M, Sari PD, Ningsih EV. Antihyperglycemic and antihyperlipidemic effects of pirdot (Saurauia vulcani Korth.) Leaves extract in mice. IOP Conf Ser: E Env Sci 2018;130:1.
16. Markand S, Saul A, Roon P, Prasad P, Martin P, Rozen R, et al. Retinal ganglion cell loss and mild vasculopathy in methylene tetrahydrofolate reductase (Mthfr)-deficient mice: A Model of mild hyperhomocysteinemia. Invest Ophthalmol Vis Sci 2015;56:2684-95.
17. Ganong FW. In: Widjajakusuma DM, editor. Textbook of Medical Physiology. Jakarta: EGC; 2002. p. 321.
18. de Gooyer TE, Stevenson KA, Humphries P, Simpson DA, Gardiner TA, Stitt AW, et al. Retinopathy is reduced during experimental diabetes in a mouse model of outer retinal degeneration. Invest Ophthalmol Vis Sci 2006;47:5561-8.
19. Robinson R, Barathi VA, Chaurasia SS, Wong TY, Kern TS. Update on animal models of diabetic retinopathy: From molecular approaches to mice and higher mammals. Dis Model Mech 2012;5:444-56.
20. Budiman A, Khoeherunnisa R, Tazyinul QA. Wound-healing test of Piper betle leaf extract and Aloe vera in gel preparation. Int J Appl Phar 2018;10:87.
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