ANTI-INFERTILITY EFFECTS TEST OF DATE PALM FRUIT EXTRACT (PHOENIX DACTYLIFERA L.) IN FEMALE MICE (MUS MUSCULUS) COMPARED WITH PROPOLIS
Objective: The date palm (Phoenix dactylifera L) is one of the plants empirically used to increase fertility. The aim of this study was to compare the effect of ethanol extract of date palm fruit and propolis on fertility in female mice.
Methods: 5 groups were assigned to 1 control and 4 experimental groups. The experimental group treated by oral administration of 100, 200 and 400 mg/kgBW of khalal date fruit extract and 200 mg/kgBW of propolis for 5, 10 and 15 days. Control groups received no extract. After 5, 10 and 15 days the mice were deeply anesthetized with anesthetic ether and sacrificed. Histological changes in ovary and uterine were measured. The data was analyzed by using two way ANOVA and Duncan test.
Results: The results of the study of the effect of the dose and duration of the ethanol extract of khalal date fruit on the histology of ovaries and uterine mice showed that there was an increase in the number of primary, secondary, tertiary, degraaf, and corpus luteum follicles but did not affect the follicle of atresia and myometrial and endometrial thickness. in Propolis a dose of 100mg / kgBW increase occurs only in primary, secondary and corpus luteum follicles.
Conclusion: Ethanol extract of khalal date fruit and Propolis can increase the number of ovarian follicles. Ethanol extract of khalal date fruit dose can increase the number of ovarian follicles higher than propolis.
2. Praveen K, Vayalil. Date fruits (Phoenix dactylifera Linn): An emerging medicinal food. Crit Rev Sci Nutr 2012;52:249-71.
3. Fazeleh M, Baharara J, Namvar F, Zafar-Balanezhad S, Amini E, Jafarzadeh E. Effect of date palm pollen on fertility and development of reproductive system in female balb/C mice. J Herbmed Pharm 2016;5:23-8.
4. Mihai C, Marghitas L, Dezmirean D, Barnutiu L. Correlation between polyphenolic profile and antioxidant activity of propolis from transylvania. Sci Pap Anim Sci Biotechnol 2011;44:100-3.
5. Sulaeman AM, Anwar F, Damanik M, Ploeger AH. Determination of Indonesian native stingless bee propolis as complementary nutraceutical candidate of anti-tuberculosis drug. Int J Pharm Pharm Sci 2018;10:15 21.
6. Ramadan A, Soliman G, Mahmoud S, Nofal S, Rahman R. Hepatoprotective and hepatotherapeutic effects of propolis against D-galactosamine/lipopolysaccharide-induced liver damage in rats. Int J Pharm Pharm Sci 2015;7:372-8.
7. El-Sharkawy E, Kames A, Sayed S, Nisr N, Wahba N, Elsherif W, et al. The ameliorative effect of propolis against methoxychlor induced ovarian toxicity in rat. Exp Toxicol Pathol 2014;66:415-21.
8. Vayalil. Date Fruits (Phoenix dactylifera Linn): An emerging medicinal food. Crit Rev Sci Nutr 2012;52:249-71.
9. Minegishi T, Nakamura K, Yamashita S, Ikeda S, Kogure K. Regulation of human luteinizing hormone receptor in ovary. Reprod Med Biol 2008;7:11-6.
10. Lewis V. Reproductive Endocrinology and Infertility. Austin: Landes Bioscience; 2007.
11. Marcucci M, Ferreres F, Viguera G, Bankova V, De Castro S, Dantas A, et al. Phenolic compounds from Brazilian propolis with pharmacological activities. J Ethnopharm 2001;74:105-20.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.