EVALUATION OF GENOTOXICITY PROFILE OF JASADA BHASMA (A ZINC-BASED MINERAL FORMULATION) IN SWISS ALBINO MICE

  • Ravi Bhasker Department of Anatomy, Krishna Institute of Medical Sciences Deemed University, Karad, Maharashtra, India.
  • Megha A Doshi Department of Anatomy, Krishna Institute of Medical Sciences Deemed University, Karad, Maharashtra, India.
  • Anjana S Department of Anatomy, Srinivas Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.
  • Ravi M Department of Pharmacology, Kodagu Institute of Medical Sciences, Kodagu, Karnataka, India.
  • Naveen Kumar Department of Anatomy, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
  • Ravi Shankar B SDM Research Centre for Ayurveda and Allied Sciences, Udupi, Karnataka, India.
  • Shakunthala R Pai Department of Anatomy, Kanachur Institute of Medical Sciences, Mangaluru Karnataka, India.
  • Savitha Hemalatha Department of Anaesthesia, Jayadeva Institute of Cardiovascular Sciences and Research, Bengaluru, Karnataka, India.

Abstract

 

 Objective: Genotoxicity is regarded as one of the potential risk factors for causing pathological diseases. It was confirmed that many chemicals have the mutagenic activity which leads to cancer. A compound which interacts with genetic material DNA and shows adverse effects by altering its structure or function is referred to as genotoxic.

Methods: The present study involved 40 Swiss albino mice weighing between 25 and 30 g body weights categorized into four different groups. Group-I (normal control) received 0.5% carboxymethyl cellulose as vehicle. Group-II (toxicant control) received 40 mg/kg/body weight cyclophosphamide on the 28th day. Group-III and IV received test drug JB 15.6 mg/kg and 78 mg/kg, respectively, for 28 consecutive days. Blood samples were collected and processed for evaluating by comet assay. The animals were sacrificed and collected the bone marrow from both the femur for chromosomal aberration and micronuclei assay.

Results: JB administered at two different dose levels did not show any significant changes in the comet assay parameters, no micronucleus was found and did not produce any chromosomal aberrations both numerically and structurally when compared to positive test control group.

Conclusion: The genotoxicity evaluation of JB did not show any chromosomal aberrations and presence of micronucleus. Thus, the safety data will refine therapeutic utility of JB encouraging their rationale use and translate into greater and broader utilization of JB.

Keywords: Jasada Bhasma, Genotoxicity, Comet assay, Chromosomal aberrations.

Author Biographies

Ravi Bhasker, Department of Anatomy, Krishna Institute of Medical Sciences Deemed University, Karad, Maharashtra, India.

PhD SCHOLOR, DEPARTMENT OF ANATOMY, KRISHNA INSTITUTE OF MEDICAL SCIENCES, DEEMED TO BE UNIVERSITY, KARAD, 

Megha A Doshi, Department of Anatomy, Krishna Institute of Medical Sciences Deemed University, Karad, Maharashtra, India.

DEPARTMENT OF ANATOMY

PROFESSOR AND HEAD

Anjana S, Department of Anatomy, Srinivas Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.

DEPARTMENT OF ANATOMY

TUTOR

Ravi M, Department of Pharmacology, Kodagu Institute of Medical Sciences, Kodagu, Karnataka, India.

PHARMACOLOGY

TUTOR

Naveen Kumar, Department of Anatomy, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.

Department of Anatomy

Assistant Professor

Ravi Shankar B, SDM Research Centre for Ayurveda and Allied Sciences, Udupi, Karnataka, India.

PHARMACOLOGY

DIRECTOR

Shakunthala R Pai, Department of Anatomy, Kanachur Institute of Medical Sciences, Mangaluru Karnataka, India.

ANATOMY

PROFESSOR AND HEAD

Savitha Hemalatha, Department of Anaesthesia, Jayadeva Institute of Cardiovascular Sciences and Research, Bengaluru, Karnataka, India.

DEPARTMENT OF ANAESTHESIA

SENIOR TECHNOLOGIST

References

1. Laura MP, Lohar R, Hajo H. The essential toxin: Impact of zinc on human health. Int J Environ Res Public Health 2010;7:1342-65.
2. Osman IF, Baumqarner A, Cemeli E, Fletcher JN, Anderson D. Genotoxicity and cytotoxicity of zinc oxide and titanium dioxide in hep-2 cells. Nanomedicine 2010;5:1193-203.
3. Priyavarat S. Kaiyyadeva Nigantu-Pathya Vibodhaka. 1st ed. Varanasi: Chaukhamba Orientalia; 1979. p. 703.
4. Priyavarat S. Kaiyyadeva Nigantu-Pathya Vibodhaka. 1st ed. Varanasi: Chaukhamba Orientalia; 1979. p. 696.
5. O’ Dell BL, Sunde RA. Handbook of Nutritionally Essential Mineral Elements. New York: Marcel Dekkar Inc.; 1997. p. 680.
6. Dev S. Ancient-modern concordance in ayurvedic plants: Some examples. Environ Health Perspect 1999;107:783-9.
7. Doshi M, Bhaskar R, Anjana S, Ravi M, Satheesha KS, Ravishankar B, et al. Evaluation of genotoxicity profile of tamra bhasma (A copper-based metallic preparation) in Swiss albino mice. Int J Anat Radiol Surg 2018;12:1-4.
8. Burlinson B, Tice RR, Speit G, Agurell E, Brendler-Schwaab SY, Collins AR, et al. Fourth international workgroup on genotoxicity testing: Results of the in vivo comet assay workgroup. Mutat Res 2007;627:31-5.
9. Mohamed S, Sabita U, Rajendra S, Raman D. Genotoxicity: Mechanisms, testing guidelines, and methods. Glob J Pharm Sci 2017;1:555-75.
10. Paget GE, Barnes JM. In: Lawrence DR, Bacharach AL, editors. Evaluation of Drug Activities: Pharmacometrics. New York: Academic Press; 1964. p. 161.
11. Hartmann A, Agurell E, Beevers C, Brendler-Schwaab S, Burlinson B, Clay P, et al. Recommendations for conducting the in vivo alkaline comet assay 4th international comet assay workshop. Mutagenesis 2003;18:45-51.
12. Allen E. Reactions of the genital tissues to estrogens. Cold Spring Harb Symp Quant Biol 1937;5:104-10. Fraser IE. The use of colchicine and colcemid for metaphase stasis in the matrix cells of wool follicles. Aust J Biol Sci 1962;16:211-7.
13. Jackson MA, Stack HF, Waters MD. Genetic activity profiles of anticancer drugs. Mutat Res 1996;355:171-208.
14. Anderson D, Bishop JB, Garner RC, Otrosky-Wegman P, Selby PB. Cyclophosphmide: Review of its mutagenecity for an assessment of potential germ cell risks. Mutat Res 1995;330:115-81.
15. Witt KL, Livanos E, Kissling GE, Torous DK, Caspary W, Tice RR. Comparison of flow cytometry-and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with non-genotoxic and genotoxic chemicals. Mutat Res 2008;649:101-3.
16. Hayashi M. The micronucleus test most widely used in vivo genotoxicity test. Genes Environ 2016;38:18.
17. Karihtala P, Soini Y, Vaskivuo L, Bloigu R, Puistola U. DNA adduct 8-hydroxydeoxyguanosine, a novel putative marker of prognostic significance in ovarian carcinoma. Int J Gynecol Cancer 2009;19:1047-51.
18. Valavanidis A, Vlachogianni T, Fiotakis C. 8-hydroxy-2 -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C 2009;27:120-39.
19. Fosmire GJ. Zinc toxicity. Am J Clin Nutr 1990;51:225-7.
20. Kim YH, Kim EY, Gwag BJ, Sohn S, Koh JY. Zinc-induced cortical neuronal death with features of apoptosis and necrosis: Mediation by free radicals. Neuroscience 1999;89:175-82.
21. Murphy JV. Intoxication following ingestion of elemental zinc. JAMA 1970;212:2119-20.
22. Powell MC, Kanarek MS. Nanomaterial health effects-part 1: Background and current knowledge. Wis Med J 2006;105:16-20.
23. Hartmann A, Agurell E, Beevers C, Brendler SS, Burlinson B. Recommendations for conducting the in-vivo alkaline comet assay. Mutagenesis 2003;18:45-51.
24. Fenech M, Kirsch VM, Natarajan AT, Surralles J, Crott J. Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis 2011;26:125-32.
25. Fenech M. Cytokinesis-block micronucleus cytome assay. Nat Protoc 2007;2:1084-104.
26. Anderson D, Plewa MJ. The international comet assay workshop. Mutagenesis 1998;13:67-73.
27. Huang Y, Chang CC, Trosko JE. Aphidicolin-induced endoreduplication in Chinese hamster cells. Cancer Res 1983;43:1362-4.
28. Locke HC. Endoreduplication in Chinese hamster cells during alpha-radiation induced G2 arrest. Mutation Res 1983;119:403-13.
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Bhasker, R., M. A. Doshi, A. S, R. M, N. Kumar, R. S. B, S. R. Pai, and S. Hemalatha. “EVALUATION OF GENOTOXICITY PROFILE OF JASADA BHASMA (A ZINC-BASED MINERAL FORMULATION) IN SWISS ALBINO MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 305-9, doi:10.22159/ajpcr.2019.v12i1.29965.
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