SOLATION, CHARACTERIZATION, AND DOCKING STUDIES OF ISOLATED COMPOUNDS AS ANTIDIABETIC MOLECULES FROM CRESSA CRETICA

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Authors

  • SANGEETA RANI Department of Pharmacognosy, S.D. College of Pharmacy and Vocational Studies, Muzaffarnagar, Uttar Pradesh, India.
  • KAVITA GAHLOT Departments of Pharmacognosy, IFTM University, Moradabad, Uttar Pradesh, India.
  • ARVIND KUMAR Department of Pharmaceutical Chemistry and Drug Design, S.D. College of Pharmacy and Vocational Studies, Muzaffarnagar, Uttar Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i4.36836

Keywords:

Cressa cretica, Spectroscopic analysis, Phytocompounds, Glycogen phosphorylase protein, Docking, Diabetes

Abstract

Objective: The purpose of this study was to investigate the diabetic effect of phytocompounds isolated from Cressa cretica Linn. using spectroscopic analysis and molecular docking studies.

Methods: Coarse powder of the whole plant of C. cretica was extracted with methanol, extracted part was subjected to silica column isolation, and two compounds: 2-Isopropyl-4-(1-methyl-dodeca-2,4-dienyloxy)-benzene-1,3,5-triol (Compound CN-01) and 11-Methyl-dodeca-2,4,6,8,10-pentenoic acid 2,3-dihydroxy-5-methyl-phenyl ester (Compound CN-02) were isolated in pure form. The three-dimensional structure of target protein was downloaded from PDB (www.rcsb.org) Protein Data Bank, Ligand file CN – 01 and CN – 02 were converted to MDL Molfile (V2000) format using ChemSketch 2017.2.1. These files could not be used directly in AutoDock 4.0 tools; thus, they were first converted to PDB files using an open babel tool.

Results: Compounds were revealed through spectroscopic analysis and screened using AutoDock 4.0 tools. Docking study recommended that CN – 01 and CN – 02 an existing phytochemical from the plant of C. cretica had the highest fitness docking score and hence could be a potent antidiabetic drug.

Conclusion: In this investigation, we docked the receptor (glycogen phosphorylase protein) holds a promising lead target formation against diabetes based on molecular docking analysis (minimum hydrogen bond length and maximum docked score). Thus, these compounds can be effectively used as drugs for treating diabetes which is predicted on the basis of docking scores.

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References

Hanrahan JE, Subroto PA, Priawan RP, Krisnamurti DG. Potential holistic preventive and therapeutic effects of Garcinia mangostana extract or isolates in Type 2 diabetes mellitus: A review. Int J Appl Pharm 2019;11:7-11.

Sudhanshu KB, Supriya K, Ashwini K, Awanish K. Antidiabetic phytoconstituents and their mode of action on metabolic pathways. Ther Adv Endocrinol Metab 2018;9:81-100.

World Health Organization. Diagnosis and Classification of Diabetes Mellitus and its Complications. Report of a WHO Consultation. Geneva: World Health Organization; 1999. p. 2.

Bruno MM, Ama M, Cacral P, Biapa N, Pauline N, Francine TN, et al. Antioxidant and synergistic antidiabetic activities of a three-plant preparation used in Cameroon folk medicine. Int Sch Res Notices 2017;2017:9501675.

Umar A, Ahmed QU, Muhammad BY, Dogarai BB, Soad SZ. Anti-hyperglycemic activity of the leaves of Tetracera scandens Linn. Merr. (Dilleniaceae) in alloxan induced diabetic rats. J Ethnopharmacol 2010;131:140-5.

Paarakh MP. In silico antidiabetic activity of limonene isolated from Coriandrum sativum Linn fruit. Acta Sci Pharm Sci 2018;2:2-6.

Menaka CT, Ravirajsinh NJ, Ansarullah T, Ranjitsinh VD, Ramachnadran AV. Prevention of high fat diet induced insulin resistance in C57BL/6J mice by Sida rhomboidea ROXB. Extract. J Health Sci 2010;56:92-8.

Amos AF, Carty MC, Zimmet P. The rising global burden of diabetes and its complications: Estimates and projections to the year 2010. Diabet Med 1997;14:S5-85.

Bibi N, Wara B, Mughal S, Morrissey H, Ball P. A critical literature review and audit of real world data to inform the relationship between mental Ill-health and Type-2 diabetes favourable prognosis. Int J Pharm Pharm Sci 2020;12:43-47.

Sunil C, Latha PG, Suja SR, Shine VJ, Shyamal S, Anuja GI. Effect of ethanolic extract of Pisonia alba Span. Leaves on blood glucose levels and histological changes in tissues of alloxan induced diabetic rats. Int J Appl Res Nat Prod 2009;2:4-11.

Kyriacou A, Ahmed AB. Exenatide use in the management of Type 2 diabetes mellitus. Pharmaceuticals 2010;3:2554-67.

Pandita R, Phadke A, Jagtap A. Antidiabetic effect of Ficus religiosa extract in streptozotocin-induced diabetic rats. J Ethnopharmacol 2010;128:462-6.

Abdallah HM, Elshamy IA, Gaber El, Gawad AM, Omer EA, Leo, MD, et al. Anti-inflammatory, antipyretic, and antinociceptive effects of a Cressa cretica aqueous extract. Planta Med 2017;83:1313-20.

Taha SE, Nagwa MA, Sahar YA, Salama M, Hanan F, Asmaa A. Cressa cretica L. Growing in Egypt: Phytochemical study and potential antioxidant and hepato-protective activities. J Appl Pharm Sci 2019;9:46-50.

Babu EN, Anusha N, Rani P. Phytochemical screening and in vivo anti-ulcer activity of methonolic extract of Cressa cretica (Linn). World J Pharm Pharm Sci 2018;7:621-8.

Afshari A, Sayyed-Alangi SZ. Antioxidant effect of leaf extracts from Cressa cretica against oxidation process in soybean oil. Food Sci Nutr 2017;5:324-33.

Shahat AA, Nazif NM, Nahla AA, Pieters S, Vlietinck AJ. Flavonoids from Cressa cretica. Qatar Univ Sci J 2005;25:72-7.

Rajan ST, Aanandhi VM. Molecular docking studies of isolated flavonoids compounds from Amaranthus tristis Linn. As alpha-amylase and alpha-glucosidase activators. Asian J Pharm Clin Res 2018;11:62-5.

Government of India, Ministry of Health and Family Welfare. The Ayurvedic Pharmacopoeia of India. 1st ed., Vol. 3, Part. 1. New Delhi: Ministry of Health and Family Welfare; 2001. p. 233-25.

Raman N. Phytochemical Composition, Mosquito Larvicidal. New Delhi: New India Publishing; 2006. p. 19-24.

Agarwal SS, Paridhavi M. Herbal Drug Technology. Hyderabad: University Press India Pvt. Ltd.; 2007.

Lamia A, Habiba L, Amel A, Francisco L, Ignacio B, Samir B, et al. Isolation, antioxidant and antimicrobial activities of ecdysteroids from Serratula cichoracea. Curr Bioact Compd 2018;14:60-6.

Raeisi S. Molecular docking studies of squalene synthase inhibitors as potential anti cardiovascular disease drugs: Insights into drug-protein interaction discovery. Pharm Sci 2013;19:39-44.

Pence HE, Williams A. Chem spider: An online chemical information resource. J Chem Educ 2010;87:1123-4.

Mohd Q, Jamal S, Gupta R, Siddiqui MH, Kesari KK, Wadhwa G, et al. Titanium dioxide nanoparticles provide protection against polycyclic aromatic hydrocarbon BaP and chrysene-induced perturbation of DNA repair machinery: A computational biology approach. Biotechnol Appl Biochem 2016;63:497-513.

Johnson LN, Barford D. A minireview on glycogen phosphorylase. J Biol Chem 1990;265:2409.

Ortmeyer HK, Bodkin NL, Hansen BC. Nature London. Am J Physiol 1989;340:E133.

Published

07-04-2020

How to Cite

SANGEETA RANI, K. GAHLOT, and A. KUMAR. “SOLATION, CHARACTERIZATION, AND DOCKING STUDIES OF ISOLATED COMPOUNDS AS ANTIDIABETIC MOLECULES FROM CRESSA CRETICA: -”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 4, Apr. 2020, pp. 84-91, doi:10.22159/ajpcr.2020.v13i4.36836.

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