ANALYSIS OF THE PHYTOCHEMICALS, ANTIOXIDANTS AND ANTIMICROBIAL ACTIVITY OF FICUS TSJAHELA BURM. F LEAF, BARK AND FRUIT EXTRACTS.
Objective: Ficus tsjahela Burm.f is a medicinal tree species, endemic to the Western Ghats having various healing properties. This study focused to examine the antioxidant and antimicrobial activities of the leaf, bark, and fruit samples of F. tsjahela.
Methods: The plant samples were subjected to soxhlet extraction for phytochemical analysis and further experimental studies. The test on phytochemical studies indicated the presence of alkaloids, saponins, glycosides, and flavanol glycosides within the plant parts respectively. The estimation of alkaloids, saponins, in vitro antioxidant and antibacterial activities revealed that the methanol bark extracts have high activity compared to others.
Results: Total alkaloid and saponin content was found to be high in leaf methanol extract was (996.17 mg/g Quinine equivalent/ g ) and (957.3 mg /g diosgenin equivalent / g) respectively In vitro antioxidant assays revealed a strong radical scavenging potential of the methanol bark extract against stable ABTS+ (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), phosphomolybdenum and superoxide radicals. Agar well diffusion method has been used to determine the antimicrobial activities against Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli, Acetobacter aceti, and Pseudomonas aeruginosa).
Conclusion: The bark methanol extract of F. tsjahela has exhibited remarkable antioxidant activity and significant antibacterial activity (P< 0.05) against all tested bacterial strains observed.
2. Kumar S, Beena AS, Awana M, Singh A. Salt-induced tissue-specific cytosine methylation downregulates expression of HKT genes in contrasting wheat (Triticum aestivum L.) genotypes. DNA. Cell Biol. 2017 Apr 1; 36(4):283-94.
3. Hatab S, Athanasio R, Holley R, Rodas?Gonzalez A, Narvaez?Bravo C. Survival and reduction of shiga toxin?producing Escherichia coli in a fresh cold?pressed juice treated with antimicrobial plant extracts. J. Food Sci. 2016, 81, 1987-95.
4. Palhares RM, Drummond MG, Brasil BD, Cosenza GP, Brandão MD, Oliveira G. Medicinal plants recommended by the world health organization: DNA barcode identification associated with chemical analyses guarantees their quality. Plos. 2015 15;10(5):e0127866.
5. Devi MR, Salam S. Wild edible plants used by the Monsang Naga tribe of Manipur, India. Pleione. 2016;10(1):90-6.
6. Kirtikar KR, Basu BD. Indian medicinal plants. Indian Medicinal Plants. 1935.
7. Gracelin DH, Britto AJ, Kumar BJ. Qualitative and quantitative analysis of phytochemicals in five Pteris species. Int J Pharm Pharm Sci. 2013; 5(1):105-7.
8. Wagner ML, Fernández T, Varela BG, Alvarez E, Ricco RA, Hajos S, Gurni AA. Anatomical, phytochemical and immunochemical studies on Ligaria cuneifolia (R. et P.) Tiegh (Loranthaceae). Pharm. Bio. 1998 1; 36(2):131-9.
9. Sreelatha S, Dinesh E, Uma C. Antioxidant properties of Rajgira (Amaranthus paniculatus) leaves and potential synergy in chemoprevention. Asian Pac. J. Cancer Prev. 2012; 13:2775-80.
10. Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997; 2(4):152-9.
11. Harborne JB. Methods of plant analysis. in Phytochem. Anal 1984 ;(1-36). Springer, Dordrecht.
12. Singh DK, Srivastava B, Sahu A. Spectrophotometric determination of Rauwolfia alkaloids: estimation of reserpine in pharmaceuticals. Anal. Sci. 2004; 20(3):571-3.
13. Makkar, H. P. S., P. Siddhuraju, and K. Becker. "A laboratory manual on quantification of plant secondary metabolites. Human Press: 2007.
14. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med.”. 1999:(9-10):1231-7.
15. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 1999:269(2):337-41.
16. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. . Anal. Biochem: 1971:44(1):276-87.
17. Perez C. Antibiotic assay by agar-well diffusion method. Acta Biol Med Exp. 1990; 15:113-5.
18. Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J. Appl. Microbiol. 1999; 86(6):985-90.
19. Sim Choi H, Woo Kim J, Cha YN, Kim C. A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells. J Immunoassay Immunochem. 2006; 27(1):31-44.
20. Tiwari V, Mishra N, Gadani K, Solanki PS, Shah NA, Tiwari M. Mechanism of anti-bacterial activity of zinc oxide nanoparticle against carbapenem-resistant Acinetobacter baumannii. Front. Microbiol. 2018: 6;9: 1218.
21. Cowan MM. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 1999 Oct 1; 12(4):564-82.
22. Mir MA, Sawhney SS, Jassal MM. Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker J. Pharm. Pharmacol2013:2(1):001-5.
23. Kumar S, Sharma UK, Sharma AK, Pandey AK. Protective efficacy of Solanum xanthocarpum root extracts against free radical damage: phytochemical analysis and antioxidant effect. Cell Mol Biol. 2012;58(1):171-8.
24. Yen GC, Duh PD. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. J. Agric. Food Chem. 1994; 42(3):629-32.
25. Huang MH, Huang SS, Wang BS, Wu CH, Sheu MJ, Hou WC, Lin SS, Huang GJ. Antioxidant and anti-inflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo. J. Ethnopharmacol. 2011; 133(2):743-50.
26. Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant Properties of the Methanol Extracts of the Leaves and Stems of Celtis africana. Rec. Nat. Prod.2009; 3(1).
27. Mokbel MS, Suganuma T. Antioxidant and antimicrobial activities of the methanol extracts from pummelo (Citrus grandis Osbeck) fruit albedo tissues. Eur. Food Res. Technol.. 2006;224(1):39-47.
28. Dixon RA. Natural products and plant disease resistance. Nature. 2001; 411(6839):843-7.
29. Dangl JL, Jones JD. Plant pathogens and integrated defence responses to infection. nature. 2001; 411(6839):826-33.
30. Barros L, Calhelha RC, Vaz JA, Ferreira IC, Baptista P, Estevinho LM. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. Eur. Food Res. Technol. 2007; 225(2):151-6.
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