ANTIMICROBIAL AND FREE RADICAL SCAVENGING ACTIVITY OF LEAF AND STEM EXTRACT OF LIMONIA ALATA WIGHT AND ARN.
Â Objective: In the present study, antioxidant activities leaves and stem of the petroleum, chloroform, acetone, and methanolic extracts from Limonia alata (L.). Leaf and stem were investigated by employing established in vitro studies. The leaves and bark are used for the fomentation of rheumatic pain; the dried fruit is useful in malignant and pestilent fevers and is used as an antidote for poisons, the folklore claim suggests that the leaf is showing wound healing property. L. alata is belonging to the family Rutaceae.
Methods: The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, superoxide radical (O2*âˆ’) scavenging activity, phosphomolybdenum assay, ferric reducing antioxidant power and metal chelating activity, was performed to know the antioxidant potency and antimicrobial studies of the plant extract of leaves and stem of L. alata.
Result: Results are evaluated higher in leaves, stem and root of L. alata (L.) recorded total phenol (59.95Â±5.30). The present state of work was designed to evaluate the phytochemical, antioxidant as well as to screen the antimicrobial present in the plant extracts of L. alata. The plant L. alata petroleum ether extract of leaf showed greater IC50 for DPPH assay (154.8 Î¼g/mL) and compare to another extract, higher reducing power activity stem in methanol (0.99EDTAE/100 g extract), better phosphomolybdenum reduction (0.512 mg/g extract) higher ferric reducing power (11542.3MmolFe(II)E/mg) extract and higher superoxide radical scavenging activity in leaf extract (332.13%). However, the better metal chelating ability was shown by the water extracts of the leaf (18.24 EDTAE/100 g) compared to other solvent extracts.
Conclusion: The result indicates the antioxidant and antibacterial activity potential of L. alata.
2. Basile A, Sorbo S, Giordano S, Ricciardi L, Ferrara S, Montesano D, et al. Antibacterial and allelopathic activity of extract from Castanea sativa leaves. Fitoterapia 2000;71:S110-6.
3. Omer ME, Elnima EI. Antimicrobial activity of Ximenia americana. Fitoterapia 2003;74:122-6.
4. Shivaprasad HN, Mohan S, Kharya MD, Shiradkar RM, Lakshman K. In vitro models for antioxidant activity evaluation: A review. Latest Rev 2005;3:4.
5. Huang DH, Chen C, Lin C, Lin Y. Antioxidant and antiproliferative activities of water spinach (Ipomoea aquatica Forsk.) constituents. Bot Bull Acad Sci 2005;46:99-106.
6. Khilfi S, Hachimi E, Khalil A, Es-Safi A, Belahyam A, Tellal A, et al. In vitro antioxidant properties of Salvia verbenaca L. Hydromethanolic extract. Indian J Pharm 2006;38:276-80.
7. Gulcin I, Alici HA, Cesur M. Determination of in-vitro antioxidant and radical scavenging activities of propofol. Pharmacol Bull 2005;53:281-5.
8. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5:1142-5.
9. Arunkumar S, Muthuselvam M. Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. World J Agric Sci 2009;5:572-6.
10. Frei B. Cardiovascular disease and nutrient antioxidants: Role of low-density lipoprotein oxidation. Crit Rev Food Sci Nutr 1995;35:83-98.
11. Halliwell B. Free radicals and antioxidants: A personal review. Nutr Rev 1997;52:253-65.
12. Liu RH. Health benefits of fruits and vegetables are from additive and synergestic combination of phytochemicals. Am J Clin Nutr 2003;78 Suppl 3:517-20.
13. Nishaa S, Vishnupriya M, Sasikumar JM, Christabel HP, Gopalakrishnan V. Antioxidant activity of ethanolic extract of Maranta arundinacea.L Tuberous Rhizomes. Asian J Pharm Clin Res 2012;5:85-8.
14. Farhan H, Rammal H, Hijazi A, Hamad H, Daher A, Reda M, et al. In vitro antioxidant activity of ethanolicand aqueous extracts from crude Malva parviflora L. grown in lebanon. Asian J Pharm Clin Res 2012;5 Suppl 3:234-8.
15. Rammal H, Bouayed J, Younos C, Soulimani R. Evidence that oxidative stress is linked to anxiety-related behaviour in mice. Brain Behav Immun 2008;22:1156-9.
16. Bunyapraphatsara N, Chokchaichareonporn A. Medicinal plants Indigenous to Thailand. Bangkok: Prachachon; 2000. p. 691.
17 Makkar HP. Quantification of Tannin in Tree and Shrub Foliage: A Laboratory Manual. Dondrecht. The Netherlands: Kluwer Academic Publishers; 2003.
18 Zhishen J, Mengecheng T, Jianming W. The determination of flavonoids contents on mulberry and their scavenging effects on superoxide radical. Food Chem 1999;64:555-9.
19 Pulido R, Bravo L, Sauro-Calixto F. Antioxident activity of dietary polyphenols as determine by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 2000;48:3396-402.
20 Blios MS. Antioxidants determination by the use of a stable free radical. Nature 1958;4617:1199-200.
21 Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161-9.
22 Beauchamp C, Fridovich I. Superoxide dismutase: Improved assay applicable to acrylaminde gels. Anal Biochem 1971;44:276-7.
23 Mojab F, Kamalinejad M, Ghaderi N, Vahidipour H. Phytochemical screening of some Iranian plants. Iran J Pharm Res 2003;3:77-82. 23.
24 Bamforth CW, Muller RE, Walker MD. Oxygen and oxygen radicals in malting and brewing: A review. J Am Soc Brew Chem 1993;53:79-88.
25 Blios MS. Antioxidant determinations by the use of a stable freeradical. Nature 1958;26:1199-200.
26 Chung KT, Wong TY, Huang YW, Lin Y. Tannins and human health: A review. Crit Rev Food Sci Nutr 1998;38:421-64.
27 Hippeli S, Elstner EF. Transition metal ion-catalyzed oxygen activation during pathogenic processes. FEBS Lett 1999;443:1-7.
28 Jadhav SJ, Nimbalkar SS, Kulkarni AD, Madhavi DL. Lipid oxidation in biological and food systems. In: Madhavi DL, Deshpande SS, Salunkhe DK, editors. Food Antioxidants. New York: Dekker Press; 1996. p. 5-63.
29 Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry 1991;20:6006-12.
30 Kulisic T, Radonic A, Katalinic V, Milos M. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 2004;85:633-40.
31 Liu H, Qiu N, Ding H, Yao R. Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Res Intern 2008;41:363-70.
32 Loo AY, Jain K, Darah I. Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food Chem 2008;107:1151-60.
33. Lu YR, Foo LY. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 2001;75:197-202.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.