• PAVITHRA S Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • SEKAR T Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.



Meliosma simplicifolia, Antioxidant, ICPMS, GC-MS, Antibacterial


Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.).

Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial.

Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity.

Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia.


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Author Biography

PAVITHRA S, Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.

Botany department

Bharathiar University



Bahramikia S, Ardestani A, Yazdanparast R. Protective effects of four Iranian medicinal plants against free radical-mediated protein oxidation. Food Chem 2009;115:37-42.

Jadhav SJ, Nimbalkar SS, Kulkarni AD. Lipid oxidation in biological and food systems. In: Madhavi DL, Deshpande SS, Salunkhe DK, editors. Food Antioxidants. New York: Dekker Press; 1996. p. 5-63.

Singh AP. Promising phytochemicals from Indian medicinal plants. Ethnobot Leaflets 2005;1:18.

Cook NC, Samman S. Flavonoids--chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem 1996;7:66-76.

Huang DJ, Chen HJ, Lin CD, Lin YH. Antioxidant and antiproliferative activities of water spinach (Ipomoea aquatica Forsk) constituents. Bot Bull Acad Sin 2005;406:99-106.

Pourmorad F, Hosseinimehr SJ, Shahabimajd N, Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. S Afr J Biotechnol 2006;5:1142-5.

Duh PD, Tu YY, Yen GC. Antioxidants activity of aqueous extract of harnjyur (Chrysanthemum morifolium Ramat). Lebensm Wiss Technol 1999;32:269-77.

Savithramma N, Rao ML, Rukmini K, Devi PS. Antimicrobial activity of silver nanoparticles synthesized by using medicinal plants. Int J Chemtech Res 2011;3:1394-402.

Kumar A, Bello B, Reichert H. Lineage-specific cell death in postembryonic brain development of Drosophila. Development 2009;136:3433-42.

Makkar HP. Quantification of Tannin in Tree and Shrub Foliage: A Laboratory Manual Dondrecht. The Netherlands: Kluwer Academic Publishers; 2003. p. 130.

Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radical. Food Chem 1999;64:555-9.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.

Chan KW, Tan GH, Wong RC. ICP-MS method validation for the analysis of trace elements in illicit heroin. Anal Lett 2012;45:1122-32.

Perez-Eid C, Pauli M, Bazerque P. An antibiotic assay by agar-well diffusion method. Acta Biol Med Exp 1990;15:113-5.

Jeong SM, Kim SY, Kim DR, Nam KC, Ahn DU. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem 2004;52:3389-93.

Loo AY, Jain K, Darah I. Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food Chem 2008;107:1151-60.

Wildman RE. Classifying nutraceuticals. In: Wildman RE, editor. Handbook of Nutraceuticals and Functional Foods. Boco Raton, USA: CRC Press; 2001. p. 13-30.



How to Cite

S, P., and S. T. “PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, AND ANTI-BACTERIAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF MELIOSMA SIMPLICIFOLIA (L.)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 14, no. 4, Apr. 2021, pp. 100-4, doi:10.22159/ajpcr.2021.v14i4.40787.



Original Article(s)