• Yogesh Murti Assistant Professor (Pharmaceutical Chemistry) Institute of Pharmaceutical Research GLA University, Mathura
  • Saurabh Sharma
  • Pradeep Mishra


Objective: Helminthiasis affects over two billion people worldwide, particularly in the tropical region. A number of medicinal plants have been used
to treat parasitic infections in man and animals. In the present study, the anthelminthic potential of Calotropis procera (Ait.) R. Br. leaves extract was
Methods: The ethanolic extract of Calotropis procera (Ait.) R. Br. leaves was separated into n-butanol and water fractions. The n-butanol fraction
was subjected to column chromatography. Ethanolic extract, n-butanol, and water fractions as well as n-hexane, chloroform, chloroform: methanol
(9:1); chromatographic elutes of n-butanol fraction were evaluated for in-vitro anthelmintic activity using Indian earthworm Pheretima posthuma as
a experimental models.
Results: The results indicated that ethanolic extract, water fraction, n-hexane, and chloroform elutes showed better activity as compared to n-butanol
fraction and chloroform: methanol (9:1) elute of Calotropis procera (Ait.) R. Br. leaves.
Conclusion: The present investigation is the scientific validation of the anthelmintic activity of C. procera (Ait.) R. Br. leaves in view of its usage in
ethnoveterinary practice. It is suggested that further research is required for identification of active principles.
Keywords: Calotropis procera, Chromatographic elutes, Pheretima posthuma, Anthelmintic activity.

Author Biography

Yogesh Murti, Assistant Professor (Pharmaceutical Chemistry) Institute of Pharmaceutical Research GLA University, Mathura

Deptt of Pharmaceutical Chemistry,

Assistant Professor 


1. Baskar L, Mazumder PM, Sasmal D, Ganguly S, Jena SS. In-vitro
anthelmintic activity of some 1-substituted imidazole derivatives. Acta
Parasitol Glob 2011;2(1):1-5.
2. Kumar VL, Arya S. Medicinal uses and pharmacological properties
of Calotropis procera. Recent Progress in Medicinal Plants. Vol. 11.
Texas, Unites State: Stadium Press; 2006.
3. Patil SH, Adkar PP, Shalker TT, Oswal RJ, Borase SP. Antidiarrhoeal
Asian J Pharm Clin Res, Vol 8, Issue 6, 2015, 188-190
Murti et al.
activity of methanolic extract of leaves of Calotropis procera R. Br. J
Pharmacol Toxicol 2011;3:16-20.
4. Murti Y, Yogi B, Pathak D. In-vitro antioxidant activity of column
chromatographic elutes of different extracts of Calotropis procera
(giant milkweed) leaves. J Pharm Res 2011;4(10):34-6.
5. Joshi R, Sharma A, Jat BL. Analysis of antioxidant activity in extract of
Calotropis procera (Ait.) R. Br. J Appl Biosci 2009;17:899-903.
6. Yesmin N, Uddin SN, Mubassara S, Ali M. Antioxidant and antibacterial
activities of Calotropis procera Linn. Am Eur J Agric Environ Sci
7. Mossa JS, Tariq M, Mohsin A, Ageel AM, al-Yahya MA, al-Said MS,
et al. Pharmacological studies on aerial parts of Calotropis procera. Am
J Chin Med 1991;19(3-4):223-31.
8. Dewan S, Sangraula H, Kumar VL. Preliminary studies on the
analgesic activity of latex of Calotropris procera. J Ethnopharmacol
9. Derald E, Edwin S, Pandlan S, Damodaran S, Jain P, Patel J.
Antimicrobial activity of leaves of Lantana camara Linn and Calotropis
procera R. Br. Indian Drugs 2008;45(9):736-7.
10. Kareem SO, Akpan I, Ojo OP. Antimicrobial activities of Calotropis
procera on selected pathogenic microorganisms. Afr J Biomed Res
11. Iwalewa EO, Elujoba AA, Bankole OA. In vitro spasmolytic effect of
aqueous extract of Calotropis procera on Guinea-pig trachea smooth
muscle chain. Fitoterapia 2005;76(2):250-3.
12. Sharma P, Sharma JD. In-vitro schizonticidal screening of Calotropis
procera. Fitoterapia 2000;71(1):77-9.
13. Sundaram S, Verma SK, Dwivedi P. In-vitro cytotoxic activity of Indian
medicinal plants used traditionally to treat cancer. Asian J Pharm Clin
Res 2011;4:27-9.
14. Verma SK, Singh SK, Mathur A. In-vitro cytotoxicity of Calotropis
procera and Trigonella foenum graecum against human cancer cell
lines. J Chem Pharm Res 2011;2(4):861-5.
15. Olaleye MT, Akindahunsi AA, Ewemade EJ. Hepatoprotective activity
of leaf-extract of Calotropis procera against acetaminophen - and
hepatitis virus-induced liver damage in rats. Niger J Nat Prod Med
16. Rahmatullah M, Sultan S, Toma TT, Lucky SA, Chowdhury MH,
Haque WM, et al. Effect of Cuscuta reflexa stem and Calotropis procera
leaf extracts on glucose tolerance in glucose-induced hyperglycemic
rats and mice. Afr J Tradit Complement Altern Med 2009;7:109-12.
17. James AB, Magbagbeola OA, Saibu GM, Oloyo A. Possible biochemical
mechanism of action of petroleum-ether extract of Calotropis procera
leaves (Asclepiadaceae) in gravid pregnant spraguedawley rats. Fed
Am Soc Exp Biol J 2009;23:879.
18. Murti Y, Yogi B, Pathak D. Pharmacognostical standardization of leaves
of Calotropis procera (Ait.) R. Br. (Asclepiadaceae). Int J Ayurveda
Res 2010;1(1):14-7.
19. Murti Y, Singh AP, Pathak D. LC-MS/MS profiling of
hydroethanolic extract of Calotropis procera leaves. iCommunique
20. Iqbal Z, Lateef M, Jabbar A, Muhammad G, Khan MN. Anthelmintic
activity of Calotropis procera (Ait.) Ait. F. flowers in sheep.
J Ethnopharmacol 2005;102:256-61.
21. Shivkar YM, Kumar VL. Anthelmintic activity of latex of Calotropis
procera. Pharm Biol 2003;41(4):263-5.
22. Al-Qarawi AA, Mahmoud OM, Sobaih, Haroun EM, Adam SE.
A preliminary study on the anthelmintic activity of Calotropis procera
latex against Haemonchus contortus infection in Najdi sheep. Vet Res
Commun 2001;25(1):61-70.
23. Kirtikar KR, Basu BD. Indian Medicinal Plants. Allahabad: Lolit
Mohan Basu; 1935.
24. Kokate CK. Practical Pharmacognosy. 4
ed. New Delhi: Vallabha
Prakashan; 1999.
25. Khandelwal KR. Practical Pharmacognosy Technique and Experiments.
ed. Pune: Nirali Prakashan; 2000.
26. Vidyarthi RD. A Textbook of Zoology. 14
ed. New Delhi:Chand and
Co., Press; 1977.
27. Thorn GW. Harrison’s Principles of Internal Medicine. New York:
McGrew Hill; 1977.
28. Vigar Z. Atlas of Medical Parasitology. 2
ed. Singapore: Publishing
House; 1984.
29. Ajaiyeoba EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic
activity of Buchholzia coriaceae and Gynandropsis gynandra extracts.
Pharm Biol 2001;39:217-20.
30. Deore SL, Khadabadi SS, Kamdi KS, Ingle VP, Kawalkar NG, Sawarkar
PS, et al. In-vitro anthelmintic activity of Cassia tora. Int J ChemTech
Res 2009;1(2):177-9.
31. Bate-Smith EC. The phenolic constituent of plants and their taxonomic
significance, dicotyledons. J Linn Soc Bot 1962;58:95-103.
32. Martin RJ. Modes of action of anthelmintic drugs. Vet J
33. Mali RG, Wadekar RR. In Vitro Anthelmintic activity of Baliospermum
montanum Muell. Arg roots. Indian J Pharm Sci 2008;70(1):131-3.
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How to Cite
Murti, Y., S. Sharma, and P. Mishra. “IN VITRO ANTHELMINTHIC ACTIVITY OF CALOTROPIS PROCERA (AIT.) R. BR. LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 6, Nov. 2015, pp. 188-90,
Original Article(s)