EFFECT OF BUTYLATED HYDROXYANISOLE ON HYDROGEN PEROXIDE INDUCED OXIDATIVE STRESS ON CEREBRAL GLIOMA CELL LINE

Abstract

Objective: Butylated hydroxyl anisole (BHA) has potential protective effects on hydrogen peroxide (H₂O₂) induced oxidative stress on cerebral glioma cell (BMG-1).The study was carried out to investigate the neuro-protective effects of BHA againstBMG-1 cerebral glioma cell in vitro.

Methods:In vitro cytotoxicity assay, Oxidative Stress Mediated Apoptosis, Determination of Oxidative Stress Indices by DCFH-DA, Effect of BHAon Extent of Lipid Peroxidation and Total GSH Levels Measurement was carried out on BMG-1 cerebral glioma cell using standard procedures.

Results: BHA was showing very less toxicity, it reached IC₅₀ value approximately equal to 200μM in 24hr treatment, decreases the distorted morphologyof the BMG-1 cells, Significant reduction of apoptotic bodies, Oxidative Stress, Lipid Peroxidation Levels and significantly increases the levels of glutathione.

Conclusion: The present study indicates the BHA may be effective in both prevention and treatmentof brain and neurodegenerative disorders in which free radical processes give a modulatory effect.