AN IMPROVED MICROMETHOD FOR THE DETERMINATION OF ACETAMINOPHEN IN PLASMA BY VISIBLE SPECTROPHOTOMETRY: APPLICATION TO A PHARMACOKINETIC STUDY IN RABBITS


Jose Raul Medina, Adela Licea, Marcela Hurtado

Abstract


Objective: To report a simple and economic alternative spectrophotometric micro-method for the determination of acetaminophen using 200 µl of plasma sample.

Methods: The analyses were based on a simple colorimetric test in which p-aminophenol, after an acidic hydrolysis step, is reacted with phenol in an alkaline medium to form an indophenol dye which exhibits a maximum extintion at 625 nm. The micro method was validated according to the international criteria, and then it was used for the quantification of plasma levels of acetaminophen after rectal administration of 100 mg of the drug to male New Zealand rabbits.

Results: The analytical recovery was 100.97%, with solutions of 5−25 µg/ml (plasma/water). The linearity with plasma solutions was good (R2 = 0.995), at the same concentration levels. The within-run and between-run precision, as well as accuracy expressed as coefficient of variation, were less than 8%. The mean accuracy was 102.24%. The stability was proved for a one-week period at −20 °C. The pharmacokinetic parameters were computed by non-linear regression analysis. The non-compartment model analysis was used to fit the in vivo data.

Conclusion: The validation parameters were within acceptable limits for clinical studies. The analysis was adequate for the determination of acetaminophen in small plasma samples, and it can be easily adapted by any hospital or clinical laboratory with non-special equipment.

 


Keywords


Acetaminophen, NSAIDs, Pharmacokinetics, Rabbits, Spectrophotometric determination

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About this article

Title

AN IMPROVED MICROMETHOD FOR THE DETERMINATION OF ACETAMINOPHEN IN PLASMA BY VISIBLE SPECTROPHOTOMETRY: APPLICATION TO A PHARMACOKINETIC STUDY IN RABBITS

Keywords

Acetaminophen, NSAIDs, Pharmacokinetics, Rabbits, Spectrophotometric determination

DOI

10.22159/ijap.2017v9i4.19246

Date

13-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 9, Issue 4, 2017 Page: 96-98

Online ISSN

0975-7058

Statistics

22 Views | 32 Downloads

Authors & Affiliations

Jose Raul Medina
Departamento Sistemas Biologicos Universidad Autonoma Metropolitana-Xochimilco
Mexico

Adela Licea
Departamento Sistemas Biologicos Universidad Autonoma Metropolitana-Xochimilco
Mexico

Marcela Hurtado
Departamento Sistemas Biologicos Universidad Autonoma Metropolitana-Xochimilco
Mexico


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