ANALYTICAL METHOD DEVELOPMENT BY SOLID PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DIPHENHYDRAMINE QUANTIFICATION IN SYRUPS

  • Marcela Hurtado Department of Biological Systems, Metropolitan Autonomous University – Xochimilco, Mexico City, Mexico
  • Jose Raul Medina Department of Biological Systems, Metropolitan Autonomous University – Xochimilco, Mexico City, Mexico

Abstract

Objective: To develop a method for the determination of diphenhydramine hydrochloride in syrups.

Methods: The developed method is based on the active ingredient recovery from the pharmaceutical formulation using a cartridge for selective solid phase extraction. This procedure is simple and fast as compared to the methods that require liquid/liquid extraction. Once the sample is eluted from the cartridge, it is injected directly into a reversed-phase chromatographic system containing a mixture of acetonitrile: 10 mmol ammonium acetate: triethylamine 60:39.5:0.5.

Results: The proposed method fulfilled the validation parameters (linearity R2>0.99, precision CV<2% and accuracy 98-102%).

Conclusion: The proposed method proved to be reliable when it was applied in the quantification of diphenhydramine hydrochloride of two commercial syrup products.

Keywords: Diphenhydramine, Pre-treatment, Solid phase extraction, Syrup

References

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How to Cite
Hurtado, M., & Medina, J. R. (2017). ANALYTICAL METHOD DEVELOPMENT BY SOLID PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DIPHENHYDRAMINE QUANTIFICATION IN SYRUPS. International Journal of Applied Pharmaceutics, 9(6), 106-109. https://doi.org/10.22159/ijap.2017v9i6.20577
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