ANALYTICAL METHOD DEVELOPMENT BY SOLID PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DIPHENHYDRAMINE QUANTIFICATION IN SYRUPS


Marcela Hurtado, Jose Raul Medina

Abstract


Objective: To develop a method for the determination of diphenhydramine hydrochloride in syrups.

Methods: The developed method is based on the active ingredient recovery from the pharmaceutical formulation using a cartridge for selective solid phase extraction. This procedure is simple and fast as compared to the methods that require liquid/liquid extraction. Once the sample is eluted from the cartridge, it is injected directly into a reversed-phase chromatographic system containing a mixture of acetonitrile: 10 mmol ammonium acetate: triethylamine 60:39.5:0.5.

Results: The proposed method fulfilled the validation parameters (linearity R2>0.99, precision CV<2% and accuracy 98-102%).

Conclusion: The proposed method proved to be reliable when it was applied in the quantification of diphenhydramine hydrochloride of two commercial syrup products.


Keywords


Diphenhydramine, Pre-treatment, Solid phase extraction, Syrup

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About this article

Title

ANALYTICAL METHOD DEVELOPMENT BY SOLID PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DIPHENHYDRAMINE QUANTIFICATION IN SYRUPS

Keywords

Diphenhydramine, Pre-treatment, Solid phase extraction, Syrup

DOI

10.22159/ijap.2017v9i6.20577

Date

08-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 9, Issue 6 (Oct-Nov), 2017 Page: 106-109

Online ISSN

0975-7058

Statistics

17 Views | 10 Downloads

Authors & Affiliations

Marcela Hurtado
Department of Biological Systems, Metropolitan Autonomous University – Xochimilco, Mexico City, Mexico
Mexico

Jose Raul Medina
Department of Biological Systems, Metropolitan Autonomous University – Xochimilco, Mexico City, Mexico


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