FORMULATION AND DEVELOPMENT OF TOPICAL ANTI ACNE FORMULATION OF SPIRULINA EXTRACT


Badduri Nihal, N. Vishal Gupta, D. V. Gowda, Manohar M.

Abstract


Objective: The objective of this research was to formulate and evaluate anti-acne ointment of C-phycocyanin(C-PC) extracted from spirulina.

Method: C-PC was successfully extracted from spirulina by using sonication and cold-maceration process. and further purified by dialysis method. By employing disc diffusion and agar dilution method, antimicrobial activity and minimum inhibitory concentration(MIC) of C-PC as determined against Propionibacterium acne(P.acne) and Staphylococcus epidermidis(S.epidermidis). Further, the two different formulationswere prepared by using water soluble and oleaginous bases and the formulations were characterized for particle size, viscosity, pH, consistency, drug diffusion, antimicrobial activity, antioxidant effect and stability studies.

Results: C-PC showed MIC value of 1.5±0.1mg/ml and 1.8±0.2mg/ml against P.acne and S.epidermidis respectively. The developed formulation had a globule diameter of 5.44mm, pH of 6.8±0.09, the viscosity of 175±0.2cps, spreadability of an 8.6±0.12g.cm/sec and had a good consistency. Both formulations were found stable among which, formulation B(FB) had maximum drug content of 95±0.6% and drug release was up to 92±0.8%.

Conclusion:  The prepared topical C-PC ointment can be successfully employed in the treatment of acneagainstP.acne and S.epidermidis.


Keywords


Acne vulgaris, antibacterial activity, MIC, spirulina, ointment, in-vitro activity

References


AslamI, Fleischer A, Feldman, S. Emerging drugs for the treatment of acne. Expert Opin. Emerging Drugs2015;20:91–101.

Vary Jr JC. Selected disorders of skin appenages-acne, alopecia, hyperhidrosis.Med Clin of North Am2016;99:1195-211.

Bhate K, Williams HC. Epidemiology of acne vulgaris. Br J Dermatol2013; 168:474-85.

Peck GL, Olsen TG, Yoder FW, Strauss JS, Downing DT, Pandya M, et al. Prolonged remissions of cystic and conglobate acne with 13-cis-retinoic acid. N Engl J Med 1979;300:329–33.

Sri AgungFK,Marline A, Bobby EV.Formulation and evaluation of anti acne gel containing citrus aurantifolia fruit juice using carbopol as gelling agent.Int J App Pharm 2018;10:147-52

Barnes LE, Levender MM, Fleischer AB, Feldman SR. Quality of life measures for acne patients. Dermatol Clin2012;30:293-300.

Goodman G. Acne and acne scarring-the case for active and early intervention. Austfam physician2006;35:503-4.

Knutsen-Larson S, Dawson AL, Dunnick CA, Dellavalle RP. Acne vulgaris: pathogenesis, treatment, and needs assessment. Dermatol Clin 2012;30:99-106.

Schnopp C, Mempel M. Acne vulgaris in children and adolescents. Minerva pediatr 2011;63:293-304.

James WD. Acne. NEngl J Med 2005;352:1463-72.

Campanella L, Russo MV, Avino P. Free and total amino acid composition in blue-green algae. AnnChim. 2002;92:343-52.

Grossman AR, Schaefer MR, Chiang GG, Collier JL. The phycobilisome, a light-harvesting complex responsive to environmental conditions. Microbiolrev1993;57:725-49.

Jaouen P, Lépine B, Rossignol N, Royer R, Quéméneur F. Clarification and concentration with membrane technology of a phycocyanin solution extracted from Spirulina platensis. Biotechnol Tech1999;13:877-81.

McCarty MF, Barroso-Aranda J, Contreras F. Oral phycocyanobilin may diminish the pathogenicity of activated brain microglia in neurodegenerative disorders. Medhypotheses. 2010;74:601-5.

Bennett A, Bogorad L. Complementary chromatic adaptation in a filamentous blue-green alga. Jcell boil 1973;58:419-35.

BoussibaS , Richmond AE. Isolation and characterization of phycocyanin from the blue-green alga Spirulina plantensis. Arch Microbiol 1979;120:155-9.

Silva LA, Kuhn KR, Moraes CC, Burkert CAV and Kalil SJ. Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis. J BrazChemSoc 2009;20: 5-12.

Liao X, Zhang B, Wang X, Yan H and Zhang X. Purification of C-Phycocyanin from Spirulina platensis by Single-Step Ion-Exchange Chromatography. Chromatographia 2012;73:291-6.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature1970;227:680.

Canadanovic‐Brunet JM, Cetkovic GS, Djilas SM, Tumbas VT, Savatović SS, Mandic AI, Markov SL, Cvetkovic DD. Radical scavenging and antimicrobial activity of horsetail (Equisetum arvense L.) extracts. IntJFood SciTechnol 2009;44:269-78.

Indian Pharmacopeias, vol. 2, appendix 11.2, A-135, (1996). Retrieved on December 20,2018http://www.who.int/medicines/publications/pharmacopoeia/WHOPSMQSM2006_2_IndexPharmacopoeiasUpdated.pdf

Vats A, Sharma P. Formulation and evaluation of topical antiacne formulation of Coriander Oil. Int J PharmPharm Sciences Research 2012;2:61-6.

.Charde YM, Sharma PH, Choudhary NG, Avari JG. Development and evaluation of herbal formulation for the treatment of acne. Int J Pharm Sci Res 2014;5:2250-60.

Rajalakshmi SV,Vinay OG.Formulation development, evaluation and optimization of medicated lip rouge containing niosomal acyclovir for the management of recurrent herpes labialis. Int J App pharm 2017;9:21-17.

Wood JH., Catacalos G, Liberman SV., Adaptation of commercial viscometer for Special application in pharmaceutical rheology-II, J Pharm Sci1963;52:375-8.

Bauer A.W. Kirby WM, Sherris JC,Truck M.Antibiotic susceptibility testing by a standardized single disc method, Am J Clinpathol1966;45:493-6.

Ahmed A, Rowshan A, Sengupta R. Formulation and in vitro/ex vivo characterization of microemulsion based hydrogel formulation of aceclofenac for topical application. Asian J Pharm Clin Res 2016;9:87-91.




Fatal error: Call to a member function getGalleyLabel() on null in /home/innowar1/public_html/journals/cache/t_compile/%%38^38D^38D7420B%%article.tpl.php on line 182