FORMULATION AND DEVELOPMENT OF TOPICAL ANTI ACNE FORMULATION OF SPIRULINA EXTRACT

  • Badduri Nihal Department of Pharmaceutics, JSS College of Pharmacy, Sri ShivarathreeshwaraNagara, Mysuru, JSS Academy of Higher Education and Research, JSS Medical Institutions Campus, Sri ShivarathreeshwaraNagara, Mysuru – 570015, Karnataka, India.
  • N. Vishal Gupta Department of Pharmaceutics, JSS College of Pharmacy, Sri ShivarathreeshwaraNagara, Mysuru, JSS Academy of Higher Education and Research, JSS Medical Institutions Campus, Sri ShivarathreeshwaraNagara, Mysuru – 570015, Karnataka, India.
  • D. V. Gowda Department of Pharmaceutics, JSS College of Pharmacy, Sri ShivarathreeshwaraNagara, Mysuru, JSS Academy of Higher Education and Research, JSS Medical Institutions Campus, Sri ShivarathreeshwaraNagara, Mysuru – 570015, Karnataka, India.
  • Manohar M. Department of Pharmaceutics, JSS College of Pharmacy, Sri ShivarathreeshwaraNagara, Mysuru, JSS Academy of Higher Education and Research, JSS Medical Institutions Campus, Sri ShivarathreeshwaraNagara, Mysuru – 570015, Karnataka, India.

Abstract

Objective: The objective of this research was to formulate and evaluate anti-acne ointment of C-phycocyanin(C-PC) extracted from spirulina.

Methods: C-PC was successfully extracted from spirulina by using sonication and cold-maceration process and further purified by dialysis method. By employing disc diffusion and agar dilution method, antimicrobial activity and minimum inhibitory concentration(MIC) of C-PC as determined against Propionibacterium acne (P. acne) and Staphylococcus epidermidis(S. epidermidis). Further, the two different formulations were prepared by using water soluble and oleaginous bases, and the formulations were characterized for particle size, viscosity, pH, consistency, drug diffusion, antimicrobial activity, and antioxidant effect and stability studies.

Results: C-PC showed MIC value of 1.5±0.1 mg/ml and 1.8±0.2 mg/ml against P. acne and S. epidermidis respectively. The developed formulation had a globule diameter of 5.44 mm, pH of 6.8±0.09, the viscosity of 175±0.2cps, spreadability of an 8.6±0.12g. cm/sec and had good consistency. Both formulations were found stable among which, formulation B(FB) had maximum drug content of 95±0.6% and drug release was up to 92±0.8%.

Conclusion: The prepared topical C-PC ointment can be successfully employed in the treatment of acneagainstP. acne and S. epidermidis.

Keywords: Acne vulgaris, Antibacterial activity, MIC, Spirulina, Ointment, In vitro activity

Author Biography

D. V. Gowda, Department of Pharmaceutics, JSS College of Pharmacy, Sri ShivarathreeshwaraNagara, Mysuru, JSS Academy of Higher Education and Research, JSS Medical Institutions Campus, Sri ShivarathreeshwaraNagara, Mysuru – 570015, Karnataka, India.
Professor

References

1. AslamI, Fleischer A, Feldman S. Emerging drugs for the treatment of acne. Expert Opin Emerging Drugs 2015;20:91–101.
2. Vary Jr JC. Selected disorders of skin appenages-acne, alopecia, hyperhidrosis. Med Clin North Am 2016;99:1195-211.
3. Bhate K, Williams HC. Epidemiology of acne vulgaris. Br J Dermatol 2013;168:474-85.
4. Peck GL, Olsen TG, Yoder FW, Strauss JS, Downing DT, Pandya M, et al. Prolonged remissions of cystic and conglobate acne with 13-cis-retinoic acid. N Engl J Med 1979;300:329–33.
5. Sri Agung FK, Marline A, Bobby EV. Formulation and evaluation of anti-acne gel containing citrus aurantifolia fruit juice using carbopol as a gelling agent. Int J Appl Pharm 2018;10:147-52.
6. Barnes LE, Levender MM, Fleischer AB, Feldman SR. Quality of life measures for acne patients. Dermatol Clin 2012; 30:293-300.
7. Goodman G. Acne and acne scarring-the case for active and early intervention. Austfam Physician 2006;35:503-4.
8. Knutsen-Larson S, Dawson AL, Dunnick CA, Dellavalle RP. Acne vulgaris: pathogenesis, treatment, and needs assessment. Dermatol Clin 2012;30:99-106.
9. Schnopp C, Mempel M. Acne vulgaris in children and adolescents. Minerva pediatr 2011;63:293-304.
10. James WD. Acne. N Engl J Med 2005;352:1463-72.
11. Campanella L, Russo MV, Avino P. Free and total amino acid composition in blue-green algae. Ann Chim 2002;92:343-52.
12. Grossman AR, Schaefer MR, Chiang GG, Collier JL. The phycobilisome, a light-harvesting complex responsive to environmental conditions. Microbiolrev 1993;57:725-49.
13. Jaouen P, Lepine B, Rossignol N, Royer R, Quemeneur F. Clarification and concentration with membrane technology of a phycocyanin solution extracted from spirulina platensis. Biotechnol Technol 1999;13:877-81.
14. McCarty MF, Barroso-Aranda J, Contreras F. Oral phycocyanobilin may diminish the pathogenicity of activated brain microglia in neurodegenerative disorders. Med hypotheses 2010;74:601-5.
15. Bennett A, Bogorad L. Complementary chromatic adaptation in a filamentous blue-green alga. J Cell Boil 1973;58:419-35.
16. Boussiba S, Richmond AE. Isolation and characterization of phycocyanin from the blue-green alga spirulina plantensis. Arch Microbiol 1979;120:155-9.
17. Silva LA, Kuhn KR, Moraes CC, Burkert CAV, Kalil SJ. Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from spirulina platensis. J Braz Chem Soc 2009;20:5-12.
18. Liao X, Zhang B, Wang X, Yan H, Zhang X. Purification of C-phycocyanin from spirulina platensis by single-step ion-exchange chromatography. Chromatographia 2012;73:291-6.
19. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680.
20. Canadanovic‐Brunet JM, Cetkovic GS, Djilas SM, Tumbas VT, Savatovic SS, Mandic AI, et al. Radical scavenging and antimicrobial activity of horsetail (Equisetum arvense L.) extracts. Int J Food Sci Technol 2009;44:269-78.
21. Indian Pharmacopeias. Vol. 2. Appendix 11.2, A-135; 1996. Available from: http://www.who.int/medicines/publications/ pharmacopoeia/WHOPSMQSM2006_2_IndexPharmacopoeiasUpdated.pdf. [Last accessed on 20 Dec 2018]
22. Vats A, Sharma P. Formulation and evaluation of topical anti-acne formulation of coriander oil. Int J Pharm Pharm Sci Res 2012;2:61-6.
23. Charde YM, Sharma PH, Choudhary NG, Avari JG. Development and evaluation of herbal formulation for the treatment of acne. Int J Pharm Sci Res 2014;5:2250-60.
24. Rajalakshmi SV, Vinay OG. Formulation development, evaluation, and optimization of medicated lip rouge containing niosomal acyclovir for the management of recurrent herpes labialis. Int J Appl Pharm 2017;9:21-17.
25. Wood JH, Catacalos G, Liberman SV. Adaptation of commercial viscometer for special application in pharmaceutical rheology-II. J Pharm Sci 1963;52:375-8.
26. Bauer AW, Kirby WM, Sherris JC, Truck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clinpathol 1966;45:493-6.
27. Ahmed A, Rowshan A, Sengupta R. Formulation and in vitro/ex vivo characterization of microemulsion based hydrogel formulation of aceclofenac for topical application. Asian J Pharm Clin Res 2016;9:87-91.
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How to Cite
Nihal, B., Gupta, N. V., Gowda, D. V., & M., M. (2018). FORMULATION AND DEVELOPMENT OF TOPICAL ANTI ACNE FORMULATION OF SPIRULINA EXTRACT. International Journal of Applied Pharmaceutics, 10(6), 229-233. https://doi.org/10.22159/ijap.2018v10i6.26334
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