Deni Rahmat, Shirly Kumala, Yunahara Farida, Benny Chandra


Objective : The aim of this study was to compare the antibacterial activity of 96% (v/v)   ethanolic extract of Javanese turmeric rhizome (Curcuma xanthorrhiza Roxb.)  and nanoparticles containing the extract against 4 pathogenic bacteria.

Methods : The synthesis of chitosan nanoparticles used ionic gelation method. The nanoparticles were evaluated for particle size, zeta potential and morphology. Dilution method was performed to determine minimum inhibitory concentration (MIC).  

Results : The nanoparticles showed particle size of 53.25 nm, polydispersity index of 0.442 and zeta potential value of +31.5 mv. The antibacterial activity results based on the broth dilution method demonstrated that both the extract and the nanoparticles could inhibit the growth of Staphylococcus aureus, Escherichia coli, Salmonella thypi, and Bacillus subtilis in a concentration of 500 µg/ml. The MIC of the extract against Staphylococcus aureus and Bacillus subtilis were 400 µg/ml  and against Escherichia coli and Salmonella thypi in a concentration of 500 µg/ml. In addition, the MIC of the nanoparticles against Staphylococcus aureus was 300 µg/ml  while against Bacillus subtilis and Salmonella thypi 400 µg/ml  and against Escherichia coli 500 µg/ml.

Conclusion : The nanoparticles have a promising strategy to formulate the extracts in order to decrease the MIC against Staphylococcus aureus and Salmonella thypi.


Javanese turmeric rhizome, extract, nanoparticles, chitosan, antibacterial activity


Salleh NAM, Ismail S, Halim MRA. Effects of Curcuma xanthorrhiza extracts and their constituents on phase ii drug metabolizing enzymes activity. Pharmacognosy Res 2016;8:309-15.

Oon SF, Nallappan M, Tee TT, Shohaini S, Kassim NK, Sa’ariwijaya MSF, et al. Xanthorrhizol: a review of its pharmacological activities and anticancer properties. Cancer Cell Int 2015;15:2-15.

Jayaprakasha GK, Rao LJM, Sakariah KK. Chemistry and biological activities of C. longa. Trends Food Sci Technol 2005;16:533-48.

Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem 2014;6:25-64.

Rai J, Randhawa GK, Kaur M. Recent advances in antibacterial drugs. Int J Appl Basic Med Res 2013;3:3-10.

Jabir NR, Tabrez S, Ashraf GM, Shakil S, Damanhouri GA, Kamal MA. Nanotechnology-based approaches in anticancer research. Int J Nanomedicine 2012;7:4391-408.

Al-nemrawi NK, Alsharif SSM, Dave RH. Preparation of chitosan-TPP nanoparticles: the influence of chitosan polymeric properties and formulation variables. Int J App Pharm 2018;10:60-5.

Wilson SC, Holder WH, Easterwood KV, Hubbard G, Johnson RH, Cooley JD, et al. Identification, remediation, and monitoring processes used in a mold-contaminated high school. Adv Appl Microbiol 2004;55:409-23.

Smith CF, Townsend DE. A new medium for determining the total plate count in food. J Food Prot 1999;62:1404-10.

Paulsen P, Schopf E, Smulders FJM. Enumeration of total aerobic bacteria and Escherichia coli in minced meat and on carcass surface samples with an automated most probable-number method compared with colony count protocols. J Food Prot 2006;69:2500-3.

Abid H, Ali J, Waqas M, Anwar Y, Ullah J. Microbial quality assessment study of branded and unbranded milk sold in Peshawar city, Pakistan. Pakistan J Nutr 2009;8:704-9.

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 2016;6:71-9.

Arullappan S, Zakaria Z, Basri DF. Preliminary screening of antibacterial activity using crude extracts of Hibiscus rosa sinensis. Trop Life Sci Res 2009;20:109-18.

Bhalodia NR, Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: an ethnomedicinal plant. J Adv Pharm Technol Res 2011;2:104-9.

United States Pharmacopeia 2011. Rockville : United States Pharmacopeial Comvention; 2010. p. 50-2.

Salzman MB, Isenberg HD, Rubin LG. Use of disinfectants to reduce microbial contamination of hubs of vascular catheters. J Clin Microbiol 1993;31:475-9.

Corkidi G, Diaz-Uribe R, Folch-Mallol JL, Nieto-Sotelo J. Covasiam: an image analysis method that allows detection of confluent microbial colonies and colonies of various sizes for automated counting. Appl Environ Microbiol 1998;64:1400-4.

Wenjing Y, Jing F, Ting W, Nongyue H. Chitosan/sodium tripolyphosphate nanoparticles: preparation, characterization and application as drug carrier. J Biomed Nanotechnol 2009;5:591-5.

Singh B, Bandopadhyay S, Kapil R, Singh R, Katare OP. Self-emulsifying drug delivery systems (sedds): formulation development, characterization, and applications. Crit Rev Ther Drug Carrier Syst 2009;26:427-521.

Niederquell A, Kuentz M. Proposal of stability categories for nano-dispersions obtained from pharmaceutical self-emulsifying formulations. Int J Pharm 2013;446:70-80.

Abouhussein DMN, El-Bary AA, Shalaby SH, El Nabarawi MA. Chitosan mucoadhesive buccal films: effect of different casting solvents on their physicochemical properties. Int J Pharm Pharm Sci 2016;8:206-13.

Sylvester WS, Son R, Lew KF, Rukayadi Y. Antibacterial activity of Java turmeric (Curcuma xanthorrhiza Roxb.) extract against Klebsiella pneumoniae isolated from several vegetables. Int Food Res J 2015;22:1770-6.

Mishra N, Behal KK. Antimicrobial activity of some spices against selected microbes. Int J Pharm Pharm Sci 2010;2:187-96.

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