• RATNA DJAMIL Laboratory of Phytochemistry, Faculty of Pharmacy, Pancasila University, Jakarta, 12640, Indonesia
  • SARAH ZAIDAN Laboratory of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta,12640, Indonesia
  • DENI RAHMAT Laboratory of Technology Pharmacy, Faculty of Pharmacy, University of Pancasila, 12640, Jakarta, Indonesia
  • DIAH KARTIKA PRATAMI Laboratory of Phytochemistry, Faculty of Pharmacy, Pancasila University, Jakarta, 12640, Indonesia
  • FELIX HAKIM Faculty of Pharmacy, University of Pancasila, 12640, Jakarta, Indonesia


Objective: Okra (Abelmoschus esculentus (L.) Moench) has potential antidiabetic activity. This study created a nanoemulsion of okra extract (NOE) and examined its activity on alloxan-induced diabetes mellitus in mice.

Methods: Okra was macerated with 70% ethanol and dried in a rotary evaporator into the crude extract. The extract was encapsulated in a solution of glyceryl caprylate, propylene glycol, and glycerine to form a nanoemulsion. To determine the antihyperglycaemic effect of okra extract, 35 male mice (Mus musculus L.) were divided into seven groups: a non-diabetic normal control group and six diabetic mice groups (untreated negative control, glibenclamide-treated positive control, and four treatments with okra ethanol extract (OEE) at 200 and 400 mg/kg BW and NOE at 200 and 400 mg/kg BW).

Results: The group treated with NOE at 400 mg/kg BW (NOE400) had the lowest average blood glucose level of 93.4 mg/dL among hyperglycaemic mice. The decrease in blood glucose levels in NOE400 (52.05%) was significantly different from those in the positive control (42.63%) and OEE treatments (39.32%). The nanoemulsion used in this study fulfills quality requirements, with a mean particle size of 134.7 nm, a polydispersity index of 0.512, and a zeta value of −26.72 mV.

Conclusion: NOE reduced blood glucose levels in alloxan-induced hyperglycaemic mice better than OEE. Nanoemulsion can improve the antidiabetic activity of okra extract by increasing penetration of active compounds into interstitial space so that their delivery and bioavailability are higher.

Keywords: Abelmoschus esculentus (L.) Moench., Alloxan-induced hyperglycaemic mice, Diabetes mellitus, Nanoparticles, Okra fruit


1. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014;37(Suppl 1):81–90.
2. Roglic G, Unwin N. Mortality attributable to diabetes: estimates for the year 2010. Diabetes Res Clin Pract 2010;87:15–9.
3. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010;87:4–14.
4. R Kokil G, V Rewatkar P, Verma A, Thareja S, R Naik S. Pharmacology and chemistry of diabetes mellitus and antidiabetic drugs: a critical review. Curr Med Chem 2010;17:4405–23.
5. Gemede HF, Ratta N, Haki GD, Woldegiorgis AZ, Bey F. Nutritional quality and health benefits of okra (Abelmoschus esculentus): a review. Pakistan J Food Sci 2015;25:16–25.
6. Sabitha V, Ramachandran S, Naveen KR, Panneerselvam K. Antidiabetic and antihyperlipidemic potential of Abelmoschus esculentus (L.) moench. in streptozotocin-induced diabetic rats. J Pharm Bioallied Sci 2011;3:397.
7. Saha D, Jain B, Jain VK. Phytochemical evaluation and characterization of hypoglycemic activity of various extracts of Abelmoschus esculentus linn. fruit. Int J Pharm Pharm Sci 2011;3:183–5.
8. Anjani PP, Damayanthi E, Rimbawan R, Handharyani E. Potential of okra (Abelmoschus esculentus L.) extract to reduce blood glucose and malondialdehyde (MDA) liver in streptozotocin-induced diabetic rats. J Gizi Dan Pangan 2018;13:47–54.
9. Suh WH, Suslick KS, Stucky GD, Suh YH. Nanotechnology, nanotoxicology, and neuroscience. Prog Neurobiol 2009;87:133–70.
10. Mohanraj VJ, Chen Y. Nanoparticles-a review. Trop J Pharm Res 2006;5:561–73.
11. Djamil R, Rahmat D, Zaidan S, Latifah MN. Anticholesterol activity of okra fruit extract (Abelmoschus esculentus (L) Moench) and its nanoemulsion in vivo. Pharmacogn J 2020;12:316–20.
12. Ditjen POM, Depkes RI. Materia medika Indonesia. Ed kelima, Jakarta Dep Kesehat RI Hal; 1995. p. 32–6.
13. Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1966;55:225–76.
14. Islam MT. Phytochemical information and pharmacological activities of okra (Abelmoschus esculentus): a literature?based review. Phyther Res 2019;33:72–80.
15. Ajmeera D, Manda S, Janapareddi K, Kolluri S. Development of nanoemulsion to improve the ocular bioavailability and patient compliance in postoperative treatment using indomethacin. Int J App Pharm 2020;12:99-107.
16. Clogston JD, Patri AK. Zeta potential measurement. In: Characterization of nanoparticles intended for drug delivery. Springer; 2011. p. 63–70.
17. Gupta PK, Pandit JK, Kumar A, Swaroop P, Gupta S. Pharmaceutical nanotechnology novel nanoemulsion-high energy emulsification preparation, evaluation, and application. Pharm Res 2010;3:117–38.
18. Sani UM. Phytochemical screening and antidiabetic effect of extracts of the seeds of Citrullus lanatus in alloxan-induced diabetic albino mice. J Appl Pharm Sci 2015;5:51–4.
19. Sharma N, Kar A. Combined effects Gymnema sylvestre and glibenclamide on alloxan-induced diabetic mice. Int J Appl Pharm 2014;6:11–4.
20. Lenzen S. The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia 2008;51:216–26.
21. Rupeshkumar M, Kavitha K, Haldar PK. Role of herbal plants in the diabetes mellitus therapy: an overview. Int J Appl Pharm 2014;6:1-3.
22. Rahmat D, Brylianto AT, Sumarny R, Kumala S, Farida Y. The effect of nanoparticles formation on the antidiabetic activity of javanese turmeric rhizome extract: the strategy to change particle size. Int J Appl Pharm 2020;10:12.
23. Narendhran S, Rajiv P, Sivaraj R. Influence of zinc oxide nanoparticles on the growth of Sesamum indicum L. in zinc-deficient soil. Int J Pharm Pharm Sci 2016;8:365-71.
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How to Cite
DJAMIL, R., ZAIDAN, S., RAHMAT, D., PRATAMI, D. K., & HAKIM, F. (2020). NANOEMULSION OF OKRA FRUIT EXTRACT AS ANTIDIABETIC TREATMENT. International Journal of Applied Pharmaceutics, 12(5), 138-142.
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