EFFECT OF NANOCURCUMIN ON CISPLATIN-INDUCED ACUTE KIDNEY INJURY: A FOCUS ON NRF2 AND KEAP1


Bashar Adi Wahyu Pandhita, Vivian Soetikno, Melva Louisa

Abstract


Objective: This study aimed to investigate the effect of nanocurcumin (NC) against cisplatin-induced acute kidney injury, focusing on Nrf2 and Keap1.
Methods: Male Sprague-Dawley rats (n=23) were divided into five groups (Control, CP, CP+Cur, CP+50 NC, and CP+100 NC) and sacrificed 7 days after
treatment. Whole kidneys were collected for reverse transcription polymerase chain reaction (RT-PCR) analysis of Nrf2 and Keap1.
Results: There were no statistically significant differences in the RT-PCR results among the groups (p>0.05). However, Keap1 expression was
upregulated in rats treated with 100 mg of NC, which may have been caused by increased Nrf2 activation and activation of a negative feedback loop,
which upregulated transcription of Keap1.
Conclusion: NC increased Keap1 levels, but not significantly.


Keywords


Cisplatin, Keap1, Nanocurcumin, Nephrotoxicity, Nrf2.

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References


Yang Y, Liu H, Liu F, Dong Z. Mitochondrial dysregulation and

protection in cisplatin nephrotoxicity. Arch Toxicol 2014;88:1249-56.

Walker RJ, Endre ZH. Cellular mechanism of drug nephrotoxicity. In:

Alpern RJ, Moe OR, Caplan MJ, editors. Seldin and Giebisch’s the

Kidney. 5th ed. Philadelphia, PA: Saunders Elsevier; 2013. p. 2889-932.

Karasawa T, Steyger PS. An integrated view of cisplatin-induced

nephrotoxicity and ototoxicity. Toxicol Lett 2015;237:219-27.

Indhumathi P, Shanmuga PE. Incidence and management of treatmentrelated

side effect for patients receiving platinum therapy in an

outpatient oncology clinic. Asian J Pharm Clin Res 2017;10:117-20.

Liu M, Grigoryev DN, Crow MT, Haas M, Yamamoto M, Reddy SP,

et al. Transcription factor nrf2 is protective during ischemic and

nephrotoxic acute kidney injury in mice. Kidney Int 2009;76:277-85.

Ma Q. Role of nrf2 in oxidative stress and toxicity. Ann Rev Pharm

Toxicol 2013;53:401-26.

Soetikno V, Sari FR, Lakshmanan AP, Arumugam S, Harima M,

Suzuki K, et al. Curcumin alleviates oxidative stress, inflammation, and

renal fibrosis in remnant kidney through the nrf2-keap1 pathway. Mol

Nutr Food Res 2013;57:1649-59.

Da’I M, Suhendi A, Meiyanto E, Jenie UA, Kawachi M. Apoptosis

induction effect of curcumin and its analogs pentagamavunon-0 and

pentagamavunon-1 on cancer cell lines. Asian J Pharma Clin Res

;10:373-6.

Shrishail D, Handral HK, Ravichandra H, Tulisianand G, Shruthi SD.

Turmeric: Nature’s precious medicine. Asian J Pharm Clin Res

;6:10-6.

Waly MI, Al Moundhri MS, Ali BH. Effect of curcumin on cisplatin- and

oxaliplatin-induced oxidative stress in human embryonic kidney (HEK)

cells. Ren Fail 2011;33:518-23.

Kuhad A, Pilkhwal S, Sharma S, Tirkey N, Chopra K. Effect of

curcumin on inflammation and oxidative stress in cisplatin-induced

experimental nephrotoxicity. J Agric Food Chem 2007;55:10150-5.

Tapia E, Soto V, Ortiz-Vega KM, Zarco-Márquez G, Molina-Jijón E,

Cristóbal-García M, et al. Curcumin induces nrf2 nuclear translocation

and prevents glomerular hypertension, hyperfiltration, oxidant stress,

and the decrease in antioxidant enzymes in 5/6 nephrectomized rats.

Oxid Med Cell Longev 2012;2012:269039.

Balogun E, Hoque M, Gong P, Killeen E, Green CJ, Foresti R, et al.

Curcumin activates the haem oxygenase-1 gene via regulation of nrf2

and the antioxidant-responsive element. Biochem J 2003;371:887-95.

Rahimi HR, Nedaeinia R, Sepehri Shamloo A, Nikdoust S,

Kazemi Oskuee R. Novel delivery system for natural products: Nanocurcumin

formulations. Avicenna J Phytomed 2016;6:383-98.




About this article

Title

EFFECT OF NANOCURCUMIN ON CISPLATIN-INDUCED ACUTE KIDNEY INJURY: A FOCUS ON NRF2 AND KEAP1

Keywords

Cisplatin, Keap1, Nanocurcumin, Nephrotoxicity, Nrf2.

DOI

10.22159/ijap.2018.v10s1.32

Date

20-12-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 10, Special Issue 1 (Dec.), 2018 Page: 152-154

Online ISSN

0975-7058

Authors & Affiliations

Bashar Adi Wahyu Pandhita
Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia.

Vivian Soetikno
Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia.

Melva Louisa
Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia.


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