IN VITRO POTENCY AND TOXICITY OF STREPTOMYCES SP. FERMENTATION PRODUCT AS AN ANTIMALARIAL THERAPY AGAINST PLASMODIUM FALCIPARUM

  • YUNI SETYANINGSIH Department of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • ABDUL LATIF Agency for the Assessment and Application of Technology, Indonesian Institute of Sciences, Jakarta, Indonesia.
  • HENDRI ASTUTY Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • DIN SYAFRUDDIN Malaria and Vector Resistance Unit, Eijkman Institute for Molecular Biology, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia.
  • PUJI BUDI SETIA ASIH Malaria and Vector Resistance Unit, Eijkman Institute for Molecular Biology, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia.

Abstract

Objective: This research aims to study the activity of a Streptomyces sp. fermentation product as an antimalarial modality in HepG2 cells.
Methods: The effects of the product against Plasmodium falciparum 3D7 were examined using an in vitro technique parasite. The potency of the
Streptomyces sp. fermentation product was examined by determining the half maximal inhibitory concentration (IC50), and the mechanism was
studied using transmission electron microscopy (TEM). Toxicity tests were also conducted.
Results: The Streptomyces sp. fermentation product had an IC50 of 0.001 μg/ml against the parasite, versus values of 0.054 and 0.022 μg/ml for
quinidine and prodigiosin, respectively. TEM revealed no formation of hemozoin. The Streptomyces sp. fermentation product was non-toxic in HepG2
cells based on its cytotoxicity concentration 50% of 1.380 μg/ml.
Conclusion: The Streptomyces sp. fermentation product has potential as a potent and non-toxic antimalarial therapy.

Keywords: Antimalarial, Streptomyces sp, Fermentation Product, Half maximal inhibitory concentration, Transmission electron microscopy, Toxicity

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How to Cite
SETYANINGSIH, Y., LATIF, A., ASTUTY, H., SYAFRUDDIN, D., & ASIH, P. B. S. (2019). IN VITRO POTENCY AND TOXICITY OF STREPTOMYCES SP. FERMENTATION PRODUCT AS AN ANTIMALARIAL THERAPY AGAINST PLASMODIUM FALCIPARUM. International Journal of Applied Pharmaceutics, 11(1), 251-255. https://doi.org/10.22159/ijap.2019.v11s1.16
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