INFLUENCE OF THE COMBINATION OF PASAK BUMI ROOT AND PROPOLIS EXTRACTS ON PARASITEMIA LEVELS IN MICE INFECTED WITH PLASMODIUM BERGHEI

NUR HASANAH; HENDRI ASTUTY

doi:10.22159/ijap.2019.v11s1.015

Authors

NUR HASANAH Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
HENDRI ASTUTY Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.015

Keywords:

Pasak bumi, Propolis, Mice, Plasmodium berghei, Parasitemia, Oral

Abstract

Objective: Malaria infection remains a global concern due to increasing resistance to artemisinin-based combination therapy. This study examined
the antimalarial effects of propolis extract alone and in combination with pasak bumi root extract.
Methods: In the study, 30 mice were divided into six groups including two control groups, two groups of mice treated with propolis alone
at concentrations of 90 and 180 mg/kg body weight (BW), and two combination groups of mice treated with 90 or 180 mg/kg BW propolis in
combination with 60 or 75 mg/kg BW pasak bumi, respectively. Plasmodium berghei 2% was injected into each mouse, and blood smears were
prepared after 8 days to assess parasitemia.
Results: The results revealed no significant difference in parasitemia levels between the positive control and the two combination groups (p=0.136 and
0.289, respectively). However, superior growth inhibition (GI) results were observed in the combination groups (97.97% and 97.83%, respectively)
than in the propolis monotherapy groups, whereas better outcomes were observed in the positive control group (98.63% GI) than in the propolis
monotherapy groups (23.88% and 51.66%, respectively).
Conclusion: These results illustrate that combination therapy is superior to propolis monotherapy in inhibiting parasitemia.

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References

1. Siciliano G, Alano P. Enlightening the malaria parasite life cycle:
Bioluminescent plasmodium in fundamental and applied research.
Front Microbiol 2015;6:391.
2. World Health Organization. World Malaria Report 2015. 3rd ed. Italy:
World Health Organization; 2015.
3. Basic Health Research (Riskesdas). Health Profile. Jakarta: Badan
Litbangkes, Ministry of Health, Republic of Indonesia; 2013.
4. Kementrian Kesehatan RI. Epidemiologi Malaria di Indonesia. Buletin
Jendela Data dan Informasi Kesehatan; 2011.
5. Ministry of Health, Republic of Indonesia. Guidelines for the Treatment
of Malaria in Indonesia; 2008.
6. World Health Organization. Guidelines for the Treatment of Malaria.
France: World Health Organization; 2015.
7. Ridzuan MA, Sow A, Rain AN, Ilham AM, Zakiah I. Eurycoma
longifolia extract-artemisinin combination: Parasitemia suppression of
Plasmodium yoelli infected mice. Trop Biomed 2007;24:111-8.
8. Mohammad M, Ali MW, Ahmad A. Modelling for extraction of major
phytochemical components from Eurycoma longifolia. J Appl Sci 2010;
10:2572-7.
9. Go ML, Liu M, Wilairat P, Rosenthal PJ, Saliba KJ, Kirk K.
Antiplasmodial chalcones inhibit sorbitol-induced hemolysis of
Plasmodium falciparum infected erythrocytes. Antimicrob Agents
Chemother 2004;48:3241-5.
10. De Castro SL. Propolis biological and pharmacological activities
therapeutic uses of this bee-product. ARBS Ann Rev Biomed 2001;
3:49-83.
11. Huang S, Zhang CP, Wang K, Li GQ, Hu FL. Recent advance in the
chemical composition of propolis. Molecules 2014;19:19610-32.
12. Parasuraman S, Raveendran R, Kesavan R. Blood sample collection in
small laboratory animal. J Pharmacol Pharmacother 2010;1:87-93.
13. Anidi IU, Servinsky LE, Rentsendorj O, Stephens RS, Scott AL,
Pearse DB. Cd36 and fyn kinase mediate malaria-induced lung
endothelial barrier dysfunction in mice infected with Plasmodium
berghei. PLoS One 2013;8:1-13.
14. Adiprameswari MP. Effectiveness of Oral Administration of a
Combination of Eurycoma longifolia and Chloquine as an Antimalarial
in Plasmodium berghei Infected Mice. [Thesis]. Faculty of Medicine,
Universitas Indonesia; 2015.
15. Olayemi KI. Therapeutic potentials of Nigerian insect-propolis
against the malaria parasit, Plasmodium berghei (Haemosporida:
Plasmodidae). Am J Drug Discov Dev 2014;4:241-7.
16. Santos VR. Propolis: Alternative Medicine for the Treatment of
Oral Microbial Diseases. Intech; 2012. Available from: http://www.
intechopen.com/books/alternative-medicine/antifungal-activity-ofpropolis-
oral-clinical-studies-in-humans. [Last cited on 2016 May 28].
17. Kaewmuangmoon J, Nonthapa P, Rattanawannee A,
Winayanuwattikun P, Chanchao C. Preliminary screening for various
bioactivities in honey and propolis extracts from Thai bees. Eur J Med
Plants 2012;2:74-92.