DETERMINATION OF AMINO ACIDS AND SUGARS CONTENT IN ANTENNARIA DIOICA GAERTN
Objective: The purpose of our study was to determine sugars and amino acids content of Antennaria dioica Gaertn. herb. In continuation of the investigation of biologically active substances from Antennaria dioica Gaertn., it advisable to study the qualitative composition and quantitative contents of sugars and amino acids from the herb of this plant.
Methods: The herb of Antennaria dioica Gaertn. was analyzed for the content of sugars by GC/MS. The amino acids were identified and quantified by HPLC method.
Results: The results of GC/MS analysis showed that in Antennaria dioica Gaertn. herb D-glucose had the highest content (7.16±0.09) mg/g, followed by D-fructose (5.27±0.06) mg/g and D-saccharose (6.72±0.08) mg/g. In the raw material a large amount of monosaccharides derivative – Myo-inositol was revealed, a content of which was (2.12±0.06) mg/g. We determined 17 bound and 16 free amino acids in the Antennaria dioica Gaertn. by HPLC method.
Conclusion: The contents of primary metabolites provide opportunities for creating medicine and food supplements. The results show that Antennaria dioica Gaertn. is a rich source of these important biologically active substances. The resulting data will be used with the further purpose to produce new drugs of natural origin.
2. Babot? M, Mocan A, Vlase L, Cris O, Ielciu I, Gheldiu AM, et al. Phytochemical analysis, antioxidant and antimicrobial activities of Helichrysum arenarium (L.) Moench. and Antennaria dioica (L.) Gaertn. flowers. Molecules 2018;23:409.
3. Erbar C, Leins P. Diversity of styles and mechanisms of secondary pollen presentation in basal Asteraceae-New insights in phylogeny and function. Flora Morphol Distrib Funct Ecol Plants 2015;217:109-30.
4. Bohm BA, Stuessy TF. Flavonoids of the Sunflower Family (Asteraceae) Springer-Verlag Wien; New York, USA: 2001. Introduction to the Sunflower Family; p. 1.
5. García-Herrera P, Sánchez-Mata MC, Cámara M, Fernández-Ruiz V, Díez-Marqués C, Molina M, et al. Nutrient composition of six wild edible Mediterranean Asteraceae plants of dietary interest. J Food Compos Anal. 2014;34:163–70.
6. Bessada SMF, Barreira JCM, Oliveira MBPP. Asteraceae species with most prominent bioactivity and their potential applications. A review Ind Crops Prod 2015;76:604–15.
7. Bayer RJ. Antennaria. Flora of North America Editorial Committee. New York: Oxford University Press; 2006. p. 388.
8. Marchyshyn S, Basaraba R, Berdey T. Investigation of phenolic compounds of Antennaria dioica (L.) Gaertn. herb. The Pharma Innov J 2017;6:09-11.
9. Semeniv DV, Belik GV. The experience of Antennaria dioica application in folk medicine and prospects of this plant use for creation of new phytohemostatics. Ukr Biopharm J 2016;6:37-41.
10. Mericli AH. Constituents of Antennaria dioica. J Nat Prod 1983;46:941.
11. Coady Y, Boylan F. Ethnopharmacology in Ireland: an overview. Braz J Pharmacogn 2014;24:197-205.
12. Abad MJ, Bedoya LM, Bermejo P. Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and their Components. Academic Press; San Diego, USA: 2013. p. 205-19.
13. Husak L, Dakhym I, Marchyshyn S, Nakonechna S. Determination of sugars and fructans content in Stachys sieboldii. Int J of Green Pharm 2018;12:70-4.
14. Chen Y, Xie MY, Wang YX, Nie SP, Li C. Analysis of the monosaccharide composition of purified polysaccharides in Ganoderma atrum by capillary gas chromatography. J Phytochem Anal 2009;20:503-10.
15. Kozachok S, Marchyshyn S, Ostapchuk A, Zavyalova L. Monosaccharide composition of Herniaria glabra L. and Herniaria polygama J. Gay. Curr Issues Pharm Med Sci 2016;29:142-44.
16. Jámbor A, Molnár-Perl I. Quantitation of amino acids in plasma by high performance liquid chromatography: Simultaneous deproteinization and derivatization with 9-fluorenylmethyloxycarbonyl chloride. J Chromatogr A 2009;1216:6218-23.
17. Hanczkó R, Jámbor A, Perl A, Molnár-Perl I. Advances in the ophthalaldehyde derivatizations: Comeback to the o-phthalaldehydeethanethiol reagent. J Chromatogr A 2007;1163:25-42.
18. Jámbor A, Molnár-Perl I. Amino acid analysis by high-performance liquid chromatography after derivatization with 9-fluorenylmethyloxycarbonyl chloride literature overview and further study. J Chromatogr A 2009;1216:3064-77.
19. Stoiko L, Dakhym I, Pokotylo O, Marchyshyn S. Polysaccharides in Centaurium erythraea Rafn. Int J Res Ayurveda Pharm 2017;8:252-5.
20. Mergenthaler P, Lindauer U, Dienel GA, Meisel A. Sugar for the brain: the role of glucose in physiological and pathological brain function. Tren Neur 2013;36:587-97.
21. Costantino D, Minozzi G, Minozzi F, Guaraldi C. Metabolic and hormonal effects of myo-inositol in women with polycystic ovary syndrome: a double-blind trial. Eur Rev Med Pharmacol Sci 2009;13:105-10.
22. Stanford Kr.I, Takahashi H, So K, Alves-Wagner AB, Prince N.B, Lehnig A.C. et al. Maternal exercise improves glucose tolerance in female offspring. Diabetes 2017;66(8):2124-36.
23. Yoshino J, Conte C, Fontana L, Mittendorfer B, Imai Sh, Schechtman K.B. et al. Resveratrol supplementation does not improve metabolic function in non-obese women with normal glucose tolerance. Cell Metab 2012;16(5):658-64.
24. Szabados L, Savoure A. Proline: a multifunctional amino acid. Trends in Pl Sci 2009;15(2):89-97.
25. Verbruggen N, Hermans C. Proline accumulation in plants: a review. Amino Acids 2008; 35:753-59.
26. Moran-Palacio EF, Tortoledo-Ortiz O, Yañez-Farias GA, Zamora-Álvarez LA, Stephens-Camacho NA, Soñanez-Organis JG et al. Determination of Amino Acids in Medicinal Plants from Southern Sonora, Mexico. Trop J Pharm Res 2014;13(4):601-6.
27. Wu G. Amino acids: metabolism, functions, and nutrition. Amino Acids 2009;37:1-17.
28. Rhoads MJ, Wu G. Glutamine, arginine, and leucine signaling in the intestine. Amino Acids 2009;37:111-22.
29. Karpyuk UV, Kislichenko VS, Gur’eva IG. HPLC Determination of free and bound amino acids in Bryonia alba. Chem of Nat Comp 2015;51(2):399-400.
30. Karpiuk UV, Abudayeh ZHM, Kyslychenko VS, Yemelianova O?. Isolation and Identification of some Primary Metabolites, Micro- and Macroelements of Aesculus hippocastanum L. Seeds. Intern J of Pharm and Phytochem Res 2017;9(1):108-13.
31. Radovick S, Margaret H, MacGillivray. Pediatric Endocrinology: A Practical Clinical Guide, Second Edition Contemporary Endocrinology, 2d ed., Springer Science & Business Media; 2013. p. 624.
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