DETERMINATION OF CARBOHYDRATES CONTENT IN GENTIANA CRUCIATA L. BY GC/MS METHOD

  • Liliia Budniak І. Horbachevsky Ternopil National Medical University
  • Liudmyla Slobodianiuk I. Horbachevsky Ternopil National Medical University
  • Svitlana Marchyshyn I. Horbachevsky Ternopil National Medical University
  • Pavlina Klepach Bukovinian State Medical University
  • Yana Honcharuk Bukovinian State Medical University

Abstract

Objective: Thus the aim of our research was to determine the qualitative composition and quantitative content of carbohydrates in the studied plant material with the prospect of its application as a medicinal plant raw material.


Methods: The carbohydrates of the herb of Gentiana cruciata L. determined by GC/MS method.


Results: The quantitative content of 4 free carbohydrates such as D-saccharose (38.39 mg/g), D-Pinitol (12.01 mg/g), D-glucose (10.05 mg/g) and D-fructose (1.69 mg/g) was established in the herb of Gentiana cruciata L. Also, this method established the qualitative composition and quantitative content of eight carbohydrates (monosaccharides and their derivatives after hydrolysis): D-glucose (29.66 mg/g), D-Pinitol (22.24 mg/g), L-arabinose (4.26 mg/g), D-galactose (3.55 mg/g), D-xylose (1.80 mg/g), L-rhamnose (1.49 mg/g), D-Dulcitol (0.76 mg/g) and D-mannose (0.44 mg/g).


Conclusion: The results of the study showed that carbohydrates from the Gentiana cruciata L. can be used as important resources of new ingredients for the pharmaceutical industry.

Keywords: Gentiana cruciata L., Carbohydrates, GC/MS, Herb

Author Biographies

Liliia Budniak, І. Horbachevsky Ternopil National Medical University

Department of Pharmacy Management, Economics and Technology

Liudmyla Slobodianiuk, I. Horbachevsky Ternopil National Medical University

Department of Pharmacognosy and Medical Botany

Svitlana Marchyshyn, I. Horbachevsky Ternopil National Medical University

I. Horbachevsky Ternopil National Medical University

Pavlina Klepach, Bukovinian State Medical University

Department of Pharmacy

Yana Honcharuk, Bukovinian State Medical University

Department of Pharmacy

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Budniak, L., Slobodianiuk, L., Marchyshyn, S., Klepach, P., & Honcharuk, Y. (2020). DETERMINATION OF CARBOHYDRATES CONTENT IN GENTIANA CRUCIATA L. BY GC/MS METHOD. International Journal of Applied Pharmaceutics, 13(1). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39820
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