OSTEOCALCIN GENE POLYMORPHISM IN INDONESIAN MEN WITH PERIODONTITIS

  • NAMIRA KAULIKA Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.
  • TALITHA ALWASRIS Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.
  • NINIARTI DJAMAL Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.
  • BENSO SULIJAYA Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.
  • CHRISTOPHER TALBOT Department of Human Genetics, Leicester University, Leicester, UK.
  • ELZA IBRAHIM AUERKARI Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.

Abstract

Objective: Through this work, we aimed to clarify the association between the severity of periodontitis and osteocalcin (OC) gene C298T polymorphism
in Indonesian male subjects.
Methods: DNA was extracted from blood serum samples of 100 consenting Indonesian men whose periodontitis was classified as mild, moderate,
or severe. Polymerase chain reaction and restriction fragment length polymorphism techniques were applied to evaluate the status of OC (C298T)
polymorphism using HindIII restriction enzyme and electrophoresis in agarose gel to separate the indicated fragments.
Results: Although genotype distribution of the OC C298T polymorphism showed a trend for more frequent occurrence of genotype hh (and allele h)
with increasing severity of periodontitis, this trend was not statistically significant in the tested sample population, in whom the HH genotype
appeared to be relatively rare.
Conclusion: The results showed no significant association between the severity of periodontitis and OC C298T polymorphism in Indonesian male
subjects.

Keywords: Osteocalcin, Gene polymorphism, Periodontitis

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How to Cite
KAULIKA, N., ALWASRIS, T., DJAMAL, N., SULIJAYA, B., TALBOT, C., & AUERKARI, E. I. (2020). OSTEOCALCIN GENE POLYMORPHISM IN INDONESIAN MEN WITH PERIODONTITIS. International Journal of Applied Pharmaceutics, 12(1), 18-20. https://doi.org/10.22159/ijap.2020.v12s1.ARL028
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