TREPONEMA DENTICOLA AND PORPHYROMONAS GINGIVALIS AS BIOINDICATOR ORAL HYGIENE STATUS AND ORGANOLEPTIC SCORE IN MOUTH BREATHING CHILDREN
Keywords:Halitosis, Mouth breathing, Oral hygiene status, Organoleptic, Porphyromonas gingivalis, Treponema denticola
Objective: Mouth breathing is a bad habit that has several impacts on dentocraniofacial growth and development in children. It also related to another
oral cavity condition, such as poor oral hygiene and halitosis. Halitosis is caused by an anaerobic bacteria product such as Treponema denticola and
Porphyromonas gingivalis. These bacteria are Gram-negative anaerobic bacteria that play a significant role to halitosis occurrence. The objective of this
study is to determine the prevalence of T. denticola and P. gingivalis as bioindicator in mouth breathing children.
Methods: A total number of 60 subjects had a mouth breathing test (19 subjects diagnosed as mouth breathers and 41 subjects as nose breathers).
Then, the subjects were classified into halitosis and oral hygiene status category. Identification of T. denticola and P. gingivalis in supragingival plaque
and buccal mucosa subjects was used a conventional polymerase chain reaction method.
Results: The correlation between Oral Hygiene Index-Simplified and organoleptic score in mouth breathers has positive correlation (r=0.001), in
the contrary, in nose breathers, it has negative correlation (r=−0.046). Meanwhile, the prevalence of T. denticola and P. gingivalis in mouth and nose
breathers has no significant differences. Moreover, the significance value of prevalence T. denticola and P. gingivalis based on clinical parameters
halitosis and oral hygiene status has no differences.
Conclusion: The prevalence of T. denticola and P. gingivalis cannot be used as bioindicator in mouth breathers.
breathers. J Pediatr (Rio J) 2019;95 Suppl 1:66-71.
2. Harari D, Redlich M, Miri S, Hamud T, Gross M. The effect
of mouth breathing versus nasal breathing on dentofacial and
craniofacial development in orthodontic patients. Laryngoscope
3. Triana CB, Ali AH, León IB. Mouth breathing and its relationship to
some oral and medical conditions: Physiopathological mechanisms
involved. Rev Habanera Cien Med 2016;15:200-12.
4. Motta LJ, Bachiega JC, Guedes CC, Laranja LT, Bussadori SK.
Association between halitosis and mouth breathing in children. Clinics
(Sao Paulo) 2011;66:939-42.
5. Villa A, Zollanvari A, Alterovitz G, Cagetti MG, Strohmenger L,
Abati S. Prevalence of halitosis in children considering oral hygiene,
gender and age. Int J Dent Hyg 2014;12:208-12.
6. Bicak DA. A current approach to halitosis and oral malodor a mini
review. Open Dent J 2018;12:322-30.
7. Saini N. Oral malodor: A common oral problem. J Bioeng Biomed Sci
8. De Geest S, Laleman I, Teughels W, Dekeyser C, Quirynen M.
Periodontal diseases as a source of halitosis: A review of the evidence
and treatment approaches for dentists and dental hygienists. Periodontol
9. Reinhardt B, Klocke A, Neering SH, Selbach S, Peters U, Flemmig TF,
et al. Microbiological dynamics of red complex bacteria following fullmouth
air polishing in periodontally healthy subjects a randomized
clinical pilot study. Clin Oral Investig 2019;23:3905-14.
10. Valen H, Biofilms SA. Biofilms and their properties. Eur J Oral Sci
11. Greene JC, Vermillion JR. The simplified oral hygiene index. J Am
Dent Assoc 1964;68:7-13.
12. Kato H, Yoshida A, Awano S, Ansai T, Takehara T. Quantitative
detection of volatile sulfur compound-producing microorganisms in
oral specimens using real-time PCR. Oral Dis 2005;11 Suppl 1:67-71.
13. Yoshida A, Suzuki N, Nakano Y, Oho T, Kawada M, Koga T.
Development of a 5’ fluorogenic nuclease-based real-time PCR assay
for quantitative detection of Actinobacillus actinomycetemcomitans
and Porphyromonas gingivalis. J Clin Microbiol 2003;41:863-6.
14. Kolenbrander PE, London J. Minireview adhere today, here tomorrow:
Oral bacterial adherence. J Bacteriol 1993;175:3247-52.
15. Tan KH, Seers CA, Dashper SG, Mitchell HL, Pyke JS, Meuric V, et al.
Porphyromonas gingivalis and Treponema denticola exhibit metabolic
symbioses. PLoS Pathog 2014;10:e1003955.
16. Lamell CW, Griffen AL, McClellan DL, Leys EJ. Acquisition and
colonization stability of Actinobacillus actinomycetemcomitans
and Porphyromonas gingivalis in children. J Clin Microbiol
17. McClellan DL, Griffen AL, Leys EJ. Age and prevalence of
Porphyromonas gingivalis in children. J Clin Microbiol 1996;34:2017-9.
18. Tanaka S, Murakami Y, Seto K, Takamori K, Yosida M, Ochiai K, et al.
The detection of Porphyromonas gingivalis, Prevotella intermedia, and
Actinobacillus actinomycetemcomitans in the supragingival plaque of
children with and without caries. Pediatr Dent 2003;25:143-8.
19. Pacheco MC, Casagrande CF, Teixeira LP, Finck NS, de Araújo
MT. Guidelines proposal for clinical recognition of mouth breathing
children. Dental Press J Orthod 2015;20:39-44.
20. Wei Y, Shi M, Zhen M, Wang C, Hu W, Nie Y, et al. Comparison
of subgingival and buccal mucosa microbiome in chronic and
aggressive periodontitis: A pilot study. Front Cell Infect Microbiol