EFFECT OF JAVANESE TURMERIC ETHANOL EXTRACT ON THE ERADICATION OF CANDIDA ALBICANS BIOFILMS IN EARLY, INTERMEDIATE, AND MATURATION PHASES
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.148Keywords:
Biofilm,, Candida albicans, Javanese turmeric ethanol extractAbstract
Objectives: The aim of this research was to observe the effect of Javanese turmeric ethanol extract (JTEE) on the eradication of Candida albicans in
various phases of biofilm development.
Methods: C. albicans biofilms were exposed to JTEE at a concentration 1–45% for 1 h. Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide assay, and the wavelength was read at 570 nm.
Results: The results showed that the 50% minimal biofilm eradication concentration was 30% in the early phase, 20% in the intermediate phase, and
25% in the maturation phase of the biofilm. The eradication percentage increased along with increasing JTEE concentration, but decreased with the
age of the biofilm.
Conclusion: We concluded that JTEE has the potential to eradicate C. albicans biofilms in various phases of development.
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Putative role of beta-1,3 glucans in Candida albicans biofilm resistance.
Antimicrob Agents Chemother 2007;51:510-20.
4. Hetrick EM, Shin JH, Paul HS, Schoenfisch MH. Anti-biofilm
efficacy of nitric oxide-releasing silica nanoparticles. Biomaterials
2009;30:2782-9.
5. Chandra J, Kuhn DM, Mukherjee PK, Hoyer LL, McCormick T,
Ghannoum MA, et al. Biofilm formation by the fungal pathogen
Candida albicans: Development, architecture, and drug resistance.
The 3rd International Conference on Global Health (ICGH), Universitas Indonesia, Bali, Indonesia 4
J Bacteriol 2001;183:5385-94.
6. Falah-Tafti A, Jafari AA, Lotfi-Kamran MH, Fallahzadeh H, Hayan RS.
A comparison of the eFficacy of nystatin and fluconazole incorporated
into tissue conditioner on the in vitro attachment and colonization of
Candida albicans. Dent Res J (Isfahan) 2010;7:18-22.
7. BPOM. National movement of drinking Curcuma zanthorrhiza. Info
POM 2005;6:1-12.
8. Rohaimi M, Halim AB, Muhammad S, Tan Z, Ismail S, Mahmud R.
Standardization and phytochemical studies of Curcuma xanthorrhiza
Roxb. Int J Pharm Pharm Sci 2012;4:606-10.
9. Oon SF, Nallappan M, Tee TT, Shohaimi S, Kassim NK,
Sa’ariwijaya MS, et al. Xanthorrhizol: A review of its pharmacological
activities and anticancer properties. Cancer Cell Int 2015;15:100.
10. Rukayadi Y, Yong D, Hwang J. In vitro anticandidal activity of
xanthorrhizol isolated from Curcuma xanthorrhiza Roxb. J Antimicrob
Chemother 2006;57:1231-4.
11. Pramudita S. Effect of Curcuma xanthorrhiza Roxb. Ethanol
Extract on Phospholipase Enzym Activity and Candida albicans
Biofilm Development. Undergraduate Thesis. Jakarta: Universitas
Indonesia; 2009.
12. Herdiantoputri RR. Antifungal Effect of Curcuma xanthorrhiza
Roxb. Ethanol Extract on Candida albicans during Filamentation
and Maturation Phases Undergraduate Thesis. Jakarta: Univeristas
Indonesia; 2015.
13. Lewiyonah R. Antifungal Effect of Curcuma xanthorrhiza Roxb.
Ethanol Extract on Candida albicans during Adhesion dan Proliferation
Phases Undergraduate Thesis. Jakarta: Universitas Indonesia; 2015.
14. Anggakusuma Y, Lee M, Hwang JK. Estrogenic activity of
xanthorrhizol isolated from Curcuma xanthorrhiza ROXB. Biol Pharm
Bull 2009;32:1892-7.
15. Fibrianty, Hatmi RU. Effect of fertilization on the quality of Curcuma
xanthorrhiza Simplicia in Kulon Progo, Yogyakarta. Balai Pengkaj
Teknol Pertani Yogyakarta 2011;22:137-41.
16. Rahardjo M. Application of standard operational procedure in Curcuma
xanthorrhiza cultivation to support it as raw materials for potential
drugs. Bull Res Herbs Med Plants 2010;9:78-93.
17. Karkowska-Kuleta J, Rapala-Kozik M, Kozik A. Fungi pathogenic
to humans: Molecular bases of virulence of Candida albicans,
Cryptococcus neoformans and Aspergillus fumigatus. Acta Biochim
Pol 2009;56:211-24.
18. Tsang PW, Bandara HM, Fong WP. Purpurin suppresses Candida
albicans biofilm formation and hyphal development. PLoS One
2012;7:e50866.
19. Brautaset T, Sletta H, Degnes KF, Sekurova ON, Bakke I, Volokhan O,
et al. New nystatin-related antifungal polyene macrolides with altered
polyol region generated via biosynthetic engineering of Streptomyces
noursei. Appl Environ Microbiol 2011;77:6636-43.
20. Henriques M, Azeredo J, Oliveira R. Candida albicans and Candida
dubliniensis: Comparison of biofilm formation in terms of biomass and
activity. Br J Biomed Sci 2006;63:5-11.
21. Rukayadi Y, Hwang JK. In vitro activity of xanthorrhizol against
Streptococcus mutans biofilms. Lett Appl Microbiol 2006;42:400-4.
Published
05-04-2019
How to Cite
BELATRIX, B., PUSPITAWATI, R., & DJAIS, A. A. (2019). EFFECT OF JAVANESE TURMERIC ETHANOL EXTRACT ON THE ERADICATION OF CANDIDA ALBICANS BIOFILMS IN EARLY, INTERMEDIATE, AND MATURATION PHASES. International Journal of Applied Pharmaceutics, 11(1), 1–4. https://doi.org/10.22159/ijap.2019.v11s1.148
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