EFFECT OF CURCUMA XANTHORRHIZA ROXB. ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS DUAL-SPECIES BIOFILMS

  • FARIDA ERVINTARI Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • RIA PUSPITAWATI Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • SRI UTAMI Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Abstract

Objective: This study aimed to determine the effect of ethanol Curcuma extract on the viability of S. mutans and Streptococcus sanguinis in a dualspecies
in vitro biofilm model.
Methods: Dual-species biofilms of S. mutans and S. sanguinis were exposed to ethanol Curcuma extract at various concentrations. The sample of
saliva was gathered from healthy volunteers. Chlorhexidine 0.2% was used as a positive control, and bacterial culture without intervention served
as a negative control. The total suspensions of 10−as were prepared for S. mutans and S. sanguinis cells. The bacteria were incubated for 20 h (active
maturation phase) and 24 h (maturation phase).
Results: The result showed decreased S. mutans and S. sanguinis viability after exposure to 0.2%–25% Curcuma ethanol extracts during the active
accumulation and maturation phases. The decrease in bacterial viability was significantly different in all concentrations of Curcuma ethanol extracts
compared with negative controls (p<0.05) in the active accumulation and maturation phases.
Conclusion: Temulawak ethanol extract (starting at 0.2%) can decrease the viability of S. mutans and S. sanguinis in a dual species in vitro biofilm
model during the accumulation and maturation phases.

Keywords: Dual species, Curuma xanthorriza ethanol extract, Streptococcus mutans, Streptococcus sanguinis, Viability

References

1. Pintauli S, Hamada T. Heading to better oral health. [Menuju gigi dan
mulut sehat]. Medan: USU Press; 2008. p. 4-8.
2. Keyes PH, Jordan HV. Factors influencing initiation, transmission and
inhibition of dental caries. In: Harris RJ, editor. Mechanisms of Hard
Tissue Destruction. New York: Academic Press; 1963. p. 261-83.
3. Samaranayake L. Essential Microbiology for Dentistry. 3rd ed.
Philadelphia. PA: Churchill Livingstone; 2006. p. 115-25.
4. Huang R, Li M, Gregory RL. Bacterial interactions in dental biofilm.
Virulence 2011;2:435-44.
5. Burmølle M, Webb JS, Rao D, Hansen LH, Sørensen SJ, Kjelleberg S,
et al. Enhanced biofilm formation and increased resistance to
antimicrobial agents and bacterial invasion are caused by synergistic
interactions in multispecies biofilms. Appl Environ Microbiol
2006;72:3916-23.
6. Prescott LM, Harley JP, Klein DA. Microbiology. Boston: Mc Graw-
Hill; 2002. p. 620-2.
7. Nisengard RJ, Newman MG. Oral Microbiology and Immunology.
2nd ed. United States of America: WB Saunders Company; 1994.
p. 34-7.
8. Angela A. Primary prevention for children with high risk dental caries
[Pencegahan primer pada anak yang berisiko karies tinggi]. Dent J (Maj
Ked Gigi) 2005;38:130-4.
9. Rahardjo MO. Implementation of operational procedure standard to
support temulawak as a potential basic material of drug [Penerapan
SOP budidaya untuk mendukung temulawak sebagai bahan baku obat
potensial]. Perspektif 2010;9:78-93.
10. National Agency of Drug and Food Control of Republic of Indoesia
[Badan Pengawas Obat Dan Makanan]. Criteria and Guideline to
Register Traditional Medicine, Standardization of Herbal Drugs,
and Phytopharmacy [Kriteria Dan Tata Laksana Pendaftaran Obat
Tradisional, Obat Herbal Terstandar Dan Fitofarmaka]. Jakarta: Badan
Table 1: Effect of Curcuma ethanol extract on the viability of Streptococcus mutans and Streptococcus sanguinis during the active
accumulation phase (20 h)
Concentration (%) Viability (%) p value to the negative control p value to the positive control
Negative control (BHI broth) 100 0.037
0.20 18.049 0.037 0.127
1 31.788 0.037 0.050
5 41.494 0.037 0.050
10 49.787 0.037 0.050
15 33.315 0.037 0.050
20 28.856 0.037 0.050
25 28.572 0.037 0.127
Positive control (chlorhexidine 0.2) 3.664 0.037
BHI: Brain heart infusion
Table 2: Effect of Curcuma ethanol extract on the viability of Streptococcus mutans and Streptococcus sanguinis during the maturation
phase (24 h)
Concentration (%) Viability (%) p value to the negative control p value to the positive control
Negative control (BHI broth) 100 0.037
0.20 24.409 0.037 0.050
1 28.925 0.037 0.050
5 45.198 0.037 0.127
10 60.646 0.037 0.050
15 59.535 0.037 0.050
20 41.793 0.037 0.513
25 33.262 0.037 0.513
Positive control (chlorhexidine 0.2) 7.671 0.037
BHI: Brain heart infusion
Table 3: Effect of Curcuma ethanol extract on the viability of Streptococcus mutans and Streptococcus sanguinis during the active
accumulation phase (20 h) and maturation phase (24 h)
Concentrations of extracts (%) Viability at active accumulation phase (%) Viability at maturation phase (%)
Negative control (BHI broth) 100 100
0.20 18.049 24.409
1 31.788 28.925
5 41.494 45.198
10 49.787 60.646
15 33.315 59.535
20 28.856 41.793
25 28.572 33.262
Positive control (chlorhexidine 0.2) 3.664 7.671
BHI: Brain heart infusion
Pengawas Obat Dan Makanan; 2005.
11. Yusron MU. Curcuma xanthorrhiza Roxb’s response towards bio
fertilizer in different condition of agroecology [Respon temulawak
(Curcuma xanthorrhiza Roxb) terhadap pemberian pupuk bio pada
kondisi agroekologi yang berbeda]. J Litt 2009;15:162-7.
12. Sari LO. The utilization of traditional medicine with considering
the advantage and the safety [Pemanfaatan obat tradisional dengan
pertimbangan manfaat dan keamanannya]. Pharm Sci Res 2012;3:1-7.
13. Mangunwardoyo W. Antimicrobial and identification of active
compound Curcuma xanthorrhiza Roxb. IJBAS-IJENS 2012;12:69-78.
14. Salleh NA, Ismail S, Halim MR. Effects of Curcuma xanthorrhiza
Extracts and their constituents on phase II drug-metabolizing enzymes
activity. Pharm Res 2016;8:309-15.
15. Utami A, Meryalita R, Prihatin AN, Ambarsari L, AsriKurniatin P,
Nurcholis W. The Variation of Curcuma xanthorrhiza Roxb ‘s Leaves
DNA Isolation [Variasi Metode Isolasi DNA Daun Temulawak
(Curcuma xanthorrhiza Roxb.)]. Bogor: Department of Biochemistry
FMIPA IPB; 2012.
16. Hwang JK, Rukayadi Y. Challenges and Opportunities in Applying
Temulawak (Curcuma xanthorrhiza Roxb.) for Industrial Oral Care
Products. Indonesia: Proceeding National Conference of National
Indonesian Chemical Association [Prosiding Seminar Nasional
Himpunan Kimia Indonesia]; 2006. p. 25-32.
17. Hertiani T, Palupi SI, Nurwindasari DH. Antimicrobial test of
Staphylococcus aureus, Eschericia coli, Shygella dysentriae and
Candida albicans from traditional medicine to infectious diseases [Uji
In vitro antimikroba terhadap Staphylococcus aureus, Eschericia coli,
Shygella dysentriae dan Candida albicans Dari Beberapa Tanaman
Obat Tradisional Untuk Penyakit Infeksi]. J Farm Indon Pharm
2003;4:89-95.
18. Parwata IM, Dewi PF. Isolation and antibacterial activity of
Alpinia galanga L [Isolasi dan uji aktivitas antibakteri minyak atsiri
dari rimpang lengkuas (Alpinia galanga L.)]. J Kimia 2008;2:100-4.
19. Kim JE, Kim HE, Hwang JK, Lee HJ, Kwon HK, Kim BI, et al.
Antibacterial characteristics of Curcuma xanthorrhiza extract on
Streptococcus mutans biofilm. J Microbiol 2008;46:228-32.
20. Rahmawati R. Antibacterial Effect of Ethanol Extract Towards Viability
of Streptococcus mutans [Efek antibakteri ekstrak etanol temulawak
terhadap viabilitas Streptococcus mutans dalam biofilm [Thesis].
Jakarta: Faculty of Dentistry, Universitas Indonesia; 2011.
21. Fidinina N. Efek Ekstrak Etanol Temulawak Terhadap Viabilitas
Streptococcus sanguinis Berdasarkan Uji Crystal Violet (in vitro)
[Thesis]: Faculty of Dentistry, Universitas Indonesia; 2011.
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How to Cite
ERVINTARI, F., PUSPITAWATI, R., & UTAMI, S. (2019). EFFECT OF CURCUMA XANTHORRHIZA ROXB. ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS DUAL-SPECIES BIOFILMS. International Journal of Applied Pharmaceutics, 11(1), 75-78. https://doi.org/10.22159/ijap.2019.v11s1.181
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Original Article(s)