STUDY OF ISOTONICITY AND OCULAR IRRITATION OF CHLORAMPHENICOLE IN SITU GEL
Context: In situ gel forming system is applied as eye drops which can undergo a transition from solution to gel. The advantage of in situ gel can be to maximize absorption of the drug in the eye and minimize drug loss before corneal penetration.
Aims: The purpose of this study was to find out the isotonicity of chloramphenicol ophthalmic in situ gel and to know the irritating effect of its in the eyes of test animals.
Settings and Design: This study were started by making four aseptic formulations of in situ gel preparations with a comparison of the baseline concentrations of different Poloxamer 407 and HPMC, F1 (5: 0.45), F2 (10: 0.45), F3 (5 : 1) and F4 (10: 1).
Methods : Four aseptic of in situ gel preparations, followed by a qualitative isotonicity test using blood cells to see the comparison between control and test preparations, and ocular irritation test using the draize test method to determine the presence or absence of the irritation.
Materials : Chloramphenicol, hydroxypropyl methylcellulose, poloxamer 407, propylene glycol, potassium dihydrogen phosphate, sodium hydroxide, ethanol and aqua bidestilata sterile.
Statistical analysis used: Descriptive
Results: The results obtained from the isotonicity test showed that blood cells that had been dripped with the test preparations were then observed under a microscope showing normal results and the results of the ocular irritation test using the draize test method showed zero values on each test parameter.
Conclusions: Chloramphenicol in situ gel are isotonic and do not cause irritation to the rabbit's eyes, so they are safe to use.
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