METHOD VALIDATION OF SIMVASTATIN IN PCL-PEG-PCL TRIBLOCK COPOLYMER MICELLES USING UV-VIS SPECTROPHOTOMETRIC FOR SOLUBILITY ENHANCEMENT ASSAY

Authors

  • DEWI PATMAYUNI Bhakti Pertiwi, School of Pharmacy Science, Palembang 30128 Indonesia
  • T. N. SAIFULLAH SULAIMAN Departement of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281 Indonesia
  • ABDUL KARIM ZULKARNAIN Departement of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281 Indonesia
  • SHAUM SHIYAN Departement of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indralaya 30622 Indonesia

DOI:

https://doi.org/10.22159/ijap.2022v14i1.42961

Keywords:

simvastatin triblock copolymer, PCL, PEG, validation, Uv-Vis spectrophotometric

Abstract

Objective: This study aims to increase the solubility of simvastatin (SIM), a hydrophobic drug, by incorporating it into PCL-PEG-PCL triblock copolymer micelles and validating the assay method used, namely Uv-Vis spectrophotometric.

Methods: The shake flask method was used to determine the increase in solubility experienced by SIM after being incorporated into the micellar system. The values ​​of maximum wavelength (λmax), linearity, LOD, LOQ, accuracy, and precision were used as parameters measured to assess the validity of the assay method used.

Results: The results showed that PCL-PEG-PCL triblock copolymer micelles could increase SIM solubility by 9.7 times (89.49±5.75 µg/ml) compared to SIM without modification (9.19±0.24 µg/ml). The validation results show the λmax value of 239 nm, a linear calibration curve with an R-value of 0.9994, LOD and LOQ of 0.33 µg/ml and 1.00 µg/ml, accurate measurement with recovery at concentrations of 80%, 100%, and 120% were 102.93±1.32%, 100.78±0.40%, and 104.58±0.79% and also had good precision ​​with RSD < 2%.

Conclusion: The PCL-PEG-PCL triblock copolymer micelles can increase SIM solubility and the Uv-Vis spectrophotometric method has been validated successfully for the quantitative analysis of SIM in PCL-PEG-PCL triblock copolymer micelles.

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Published

02-11-2021

How to Cite

PATMAYUNI, D., SULAIMAN, T. N. S., ZULKARNAIN, A. K., & SHIYAN, S. (2021). METHOD VALIDATION OF SIMVASTATIN IN PCL-PEG-PCL TRIBLOCK COPOLYMER MICELLES USING UV-VIS SPECTROPHOTOMETRIC FOR SOLUBILITY ENHANCEMENT ASSAY. International Journal of Applied Pharmaceutics, 14(1). https://doi.org/10.22159/ijap.2022v14i1.42961

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