OPTIMIZATION OF A NOVEL KINETIC-ASSISTED INFUNDATION FOR RICH-EGCG AND POLYPHENOLS OF WHITE TEA (CAMELLIA SINENSIS) USING CENTRAL COMPOSITE DESIGN


Shaum Shiyan, Triana Hertiani, Ronny Martien, Akhmad Kharis Nugroho

Abstract


Objective: This research aimed to find modeling and optimization of a novel kinetic-assisted infundation for rich-epigallocatechin gallate (EGCG) and polyphenols extraction from white tea leaf (Camellia sinensis L.).

Methods: The optimal conditions for the best extraction of kinetic-assisted infundation were determined using central composite design. The content of EGCG, catechin, gallic acid, caffeine, theobromine, and theophylline was quantified using high-performance liquid chromatography with ultraviolet detection (HPLC/UV-detection). The total polyphenolic content (TPC) and total flavonoid content (TFC) was measured using the spectrophotometric method. The semi-purified extract was characterized by HPLC, fourier transform infrared spectrophotometry-universal attenuated total reflectance (FTIR-UATR), and powder-X ray diffraction (P-XRD). The extraction mechanism of target compounds was analyzed using scanning electron microscopy (SEM) qualitatively.

Results: The optimum process for the rich-EGCG and polyphenolic extraction using kinetic-assisted infundation was the concentration of simplicia 14.75 %, the stir rate 440 rpm, and extraction time 12.31 min. The yield of extracts, TPC, TFC, EGCG, caffeine, gallic acid, and theobromine contents in the optimal extraction process were 9.34 %, 70.97 %, 13.95 %, 29.02 %, 11.95 %, 1.33 %, and 0.33 %, respectively.

Conclusion: The kinetic-assisted infundation method proved to be easy to apply with good results as an alternative technique for extracting polyphenolic compounds and rich-EGCG from white tea leaves.


Keywords


White tea, Central composite design, Polyphenols, Kinetic-assisted infundation, Epigallocatechin gallate

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About this article

Title

OPTIMIZATION OF A NOVEL KINETIC-ASSISTED INFUNDATION FOR RICH-EGCG AND POLYPHENOLS OF WHITE TEA (CAMELLIA SINENSIS) USING CENTRAL COMPOSITE DESIGN

Keywords

White tea, Central composite design, Polyphenols, Kinetic-assisted infundation, Epigallocatechin gallate

DOI

10.22159/ijap.2018v10i6.29654

Date

04-10-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Articles In Press [Scheduled in Nov-Dec 2018] Page: 259-267

Online ISSN

0975-7058

Authors & Affiliations

Shaum Shiyan
1) Programme of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas. Sriwijaya, Indralaya, Sumatera Selatan, Indonesia 2) Faculty of Pharmacy, Universitas Gadjah Mada
Indonesia

Triana Hertiani
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, D.I. Yogyakarta, Indonesia.
Indonesia

Ronny Martien
Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, D.I. Yogyakarta, Indonesia.
Indonesia

Akhmad Kharis Nugroho
Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, D.I. Yogyakarta, Indonesia.
Indonesia


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