CENTRAL COMPOSITE DESIGN FOR OPTIMIZING EXTRACTION OF EGCG FROM GREEN TEA LEAF (CAMELLIA SINENSIS L.)


Eka Indra Setyawan, Erna Prawita Setyowati, Abdul Rohman, Akhmad Kharis Nugroho

Abstract


Objective: This study was intended to optimize the extraction condition using central composite design.

Methods: Central composite cesign with three independent variables, namely water temperature, brewing time, and brewing number were used to obtain the optimum extraction condition. Two dependent variables, namely yield of extraction and epigallocatechingallate level were used as a response parameter. Epigallocatechin gallate level was determined by using high-performance liquid chromatography method.

Results: Extraction yield was varied from 0.30 g to 0.72 g. All variables, namely water temperature, brewing time, and brewing number were able to increase the extraction yield. Epigallocatechingallate level was varied from 190.23 mg/g to 301.74 mg/g. Water temperature, brewing time, and both interaction were able to increase the epigallocatechin gallate level in green tea extract.

Conclusion: Optimum extraction condition was shown using hot water at a temperature of 95 °C for 20 min and two-times infusions. The condition obtained extraction yield and epigallocatechingallate of 0.70 g and 286.87 mg/g dry weight, respectively.


Keywords


Green tea, Extraction, Epigallocatechingallate, Optimization, Central composite design

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About this article

Title

CENTRAL COMPOSITE DESIGN FOR OPTIMIZING EXTRACTION OF EGCG FROM GREEN TEA LEAF (CAMELLIA SINENSIS L.)

Keywords

Green tea, Extraction, Epigallocatechingallate, Optimization, Central composite design

DOI

10.22159/ijap.2018v10i6.29245

Date

21-09-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Articles In Press [Scheduled in Nov-Dec 2018] Page: 211-216

Online ISSN

0975-7058

Authors & Affiliations

Eka Indra Setyawan
Department of Pharmacy, Faculty of Mathematics and Natural Science, University of Udayana, Bali, Indonesia.
Indonesia

Erna Prawita Setyowati
Departement of Pharmaceutical Biology, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
Indonesia

Abdul Rohman
Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
Indonesia

Akhmad Kharis Nugroho
Departement of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
Indonesia


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