RAPID AUTHENTICATION OF TURMERIC POWDER ADULTERATED WITH CURCUMA ZEDOARIA AND CURCUMA XANTHORRHIZA USING FTIR-ATR SPECTROSCOPY AND CHEMOMETRICS

  • ELLSYA ANGELINE Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia
  • RATNA ASMAH SUSIDARTI Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia
  • ABDUL ROHMAN Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

Abstract

Objective: The objective of this study is to develop a rapid, simple, non-destructive and inexpensive analytical method using Fourier Transform Infrared (FTIR) spectroscopy with Attenuated Total Reflection (ATR) as a sampling technique, combined with chemometrics for authentication of turmeric powder adulterated with Curcuma zedoaria and Curcuma xanthorrhiza.


Methods: Turmeric powder is placed above the diamond crystal in ATR compartment. Spectra are scanned in the absorbance mode from 4000 to 600 cm-1. The obtained spectra is further analyzed by Principal Component Analysis (PCA), Partial Least Square Discriminant Analysis (PLS-DA), and Partial Least Square Regression (PLS-R).


Results: PCA score plot shows that Curcuma longa, Curcuma zedoaria, and Curcuma xanthorrhiza can be discriminated well. PLS-DA can be used to build the model for classification between pure turmeric powder and adulterated powder with the values of Q2, R2X, and R2Y of 0.9558, 0.9813, and 0.9746, respectively. The good calibration model for quantification of each adulterant is obtained by PLS-R with R2 value more than 0.99 and lower RMSEC value. Both models have been validated by internal and external validation which result in the high R2 value and low RMSEP value which indicates that both models are accurate and precise.


Conclusion: The combination of FTIR-ATR spectroscopy and chemometrics can be used to authenticate turmeric powder adulterated with Curcuma zedoaria and Curcuma xanthorrhiza.

Keywords: Curcuma longa, Curcuma zedoaria, Curcuma xanthorrhiza, FTIR spectroscopy, Chemometrics

References

1. Pothitirat W, Gritsanapan W. Quantitative analysis of curcumin, demethoxycurcumin and bisdemethoxycurcumin in the crude curcuminoid extract from Curcuma longa in Thailand by TLC-densitometry. Mahidol Univ J Pharm Sci 2005;32:23–30.
2. Lim HS, Park SH, Ghafoor K, Hwang SY, Park J. Quality and antioxidant properties of bread containing turmeric (Curcuma longa L.) cultivated in South Korea. Food Chem 2011;124:1577–82.
3. Khan MA, El-Khatib R, Rainsford KD, Whitehouse MW. Synthesis and anti-inflammatory properties of some aromatic and heterocyclic aromatic curcuminoids. Bioorganic Chem 2012;40:30–8.
4. Changtam C, de Koning HP, Ibrahim H, Sajid MS, Gould MK, Suksamrarn A. Curcuminoid analogs with potent activity against trypanosoma and leishmania species. Eur J Med Chem 2010;45:941–56.
5. Dao TT, Nguyen PH, Won HK, Kim EH, Park JS, Won BY, et al. Curcuminoids from curcuma longa and their inhibitory activities on influenza a neuraminidases. Food Chem 2012;134:21–8.
6. Paramasivam M, Poi R, Banerjee H, Bandyopadhyay A. High-performance thin layer chromatographic method for quantitative determination of curcuminoids in curcuma longa germplasm. Food Chem 2009;113:640-4.
7. Rafi M, Wulansari L, Heryanto R, Darusman LK, Lim LW, Takeuchi T. Curcuminoid’s content and fingerprint analysis for authentication and discrimination of curcuma xanthorrhiza from curcuma longa by high-performance liquid chromatography-diode array detector. Food Anal Methods 2015;8:2185–93.
8. Rafi M, Rohaeti E, Miftahudin A, Darusman LK. Differentiation of Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar by thin layer chromatography fingerprinting analysis. Indones J Chem 2011;11:71–4.
9. Parvathy VA, Swetha VP, Sheeja TE, Sasikumar B. Detection of plant-based adulterants in turmeric powder using DNA barcoding. Pharm Biol 2015;53:1774–9.
10. Windarsih A, Rohman A, Swasono RT. Authentication of turmeric using proton-nuclear magnetic resonance spectroscopy and multivariate analysis. Int J Appl Pharm 2018;10:174-9.
11. Sasikumar B, Syamkumar S, Remya R, John Zachariah T. PCR based detection of adulteration in the market samples of turmeric powder. Food Biotechnol 2004;18:299–306.
12. Dhanya K, Syamkumar S, Siju S, Sasikumar B. Sequence characterized amplified region markers: A reliable tool for adulterant detection in turmeric powder. Food Res Int 2011;44:2889–95.
13. Rodriguez Saona LE, Giusti MM, Shotts M. Advances in infrared spectroscopy for food authenticity testing, in advances in food authenticity testing, Elsevier; 2016. p. 71–116.
14. Biancolillo A, Marini F. Chemometrics applied to plant spectral analysis, in Comprehensive Analytical Chemistry; 2018. p. 69–104.
15. Brereton RG. Applied chemometrics for scientists, John Wiley and Sons; 2013. p. 397.
16. Ballabio D, Consonni V. Classification tools in chemistry. Part 1: linear models. PLS-DA. Anal Methods 2013;5:3790.
17. Miller JN, Miller JC. Statistics and chemometrics for analytical chemistry. 6ed Harlow: Prentice Hall; 2010.
18. Rohaeti E, Rafi M, Syafitri UD, Heryanto R. Fourier transform infrared spectroscopy combined with chemometrics for discrimination of Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar. Spectrochim Acta A: Mol Biomol Spectrosc 2015;137;1244–9.
Statistics
22 Views | Downloads
How to Cite
ANGELINE, E., SUSIDARTI, R. A., & ROHMAN, A. (2019). RAPID AUTHENTICATION OF TURMERIC POWDER ADULTERATED WITH CURCUMA ZEDOARIA AND CURCUMA XANTHORRHIZA USING FTIR-ATR SPECTROSCOPY AND CHEMOMETRICS. International Journal of Applied Pharmaceutics, 11(5). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/33701
Section
Original Article(s)