QUANTITATIVE ANALYSISOF STEVIOSIDE AND REBAUDIOSIDE A IN STEVIAREBAUDIANA LEAVES USING INFRARED SPECTROSCOPY AND MULTIVARIATE CALIBRATION


Yohannes Martono, Abdul Rohman, Abdul Rohman

Abstract


Objective: The objective of this research was to develop Fourier transform infrared (FTIR)spectroscopy in combination with multivariate analysis of partial least square (PLS) regression for quantitative analysis of stevioside and rebaudioside A in S. rebaudiana leaves extract.

Methods: Stevia rebaudiana leaves with various ages were obtained from several high hills in Central Java, Indonesia. The extract samples were scanned using FTIR spectrophotometer in wavenumbers region of 4000 – 650 cm-1. PLS calibration model was established by plotting the actual value of stevioside and rebaudioside A as determined by high performance liquid chromatography (HPLC) and FTIR predicted value. The performance of PLS regression was evaluated using coefficient determination (R2), root mean square error of calibration (RMSEC), root mean square error of prediction (RMSEP).

Results: PLS regression for stevioside determination was successfully established usingthe combined wavenumbersregion of 671–1450 and 3279-3301 cm-1. PLS regression revealed R2of 0.9952with RMSEC value of0.84%. Meanwhile, rebaudioside A was determined at wavenumbersregion of 921–1508 cm-1using normal spectra. PLS model revealed R2 and RMSEC of 0.9911 and 0.70%, respectively.

Conclusion: FTIR spectroscopy in combination with multivariate analysis of PLS regression could be used an alternative method for quantitative analysis ofstevioside and rebaudioside A in S. rebaudiana leaves.


Keywords


FTIR spectroscopy, Partial least square regression, Stevia rebaudiana, Stevioside, Rebaudioside A

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