• Anjar Windarsih Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
  • Abdul Rohman Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
  • Respati Tri Swasono Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta



1H-NMR spectroscopy, turmeric, authentication, PLS-DA, OPLS-DA, PLS


  1. Objective: The objective of this study was to apply 1H-NMR spectroscopy-based metabolite fingerprinting in combination with multivariate analysis for authentication of turmeric (Curcuma longa) from C. heyneana and C. manga.

    Methods: Partial least square-discriminant analysis (PLS-DA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) were used for differentiation of authentic and adulterated C. longa with C. manga and C. heyneana. The variables used were peaks with certain chemical shifts at optimized 1H-NMR spectra of authentic and adulterated C. longa.

    Results: All of the authentic C. longa samples were clearly separated from the adulterated ones. The multivariate calibration of partial least square (PLS) was successfully applied to predict of adulterants in C. longa. The lower RMSEC (root mean square error of calibration) values, 0.94% for adulterated C. longa with C. heyneana and 1.37% for adulterated C. longa with C. manga, and the lower RMSEP (root mean square error of prediction) values, 0.83% for adulterated C. longa with C. heyneana and 1.34% for adulterated C. longa with C. manga indicated the good of accuracy and precision of the calibration models.

    Conclusion: The combination of 1H-NMR spectroscopy and chemometrics of multivariate analysis PLS-DA, OPLS-DA, and PLS proves an adequate technique for authentication of turmeric.


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How to Cite

Windarsih, A., Rohman, A., & Swasono, R. T. (2018). AUTHENTICATION OF TURMERIC USING PROTON-NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND MULTIVARIATE ANALYSIS. International Journal of Applied Pharmaceutics, 10(6), 174–180.



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