FORMULATION AND CHARACTERIZATION OF TIMOLOL MALEATE-LOADED NANOPARTICLES GEL BY IONIC GELATION METHOD USING CHITOSAN AND SODIUM ALGINATE
Objectives: The objectives of this study were to formulate and characterize nanoparticle gel of timolol maleate by ionic gelation method using chitosan and sodium alginate.
Methods: Optimization was carried out by factorial design using Design Expert® 10.0.1 software to obtain the concentration of chitosan (CS), sodium alginate (SA), and calcium chloride (CaCl2) to produce the optimum formula of TM nanoparticles. The optimum formula was characterized for particle size, polydispersity index, entrapment efficiency, Zeta potential, and molecular structure. Hydroxy Propyl Methyl Cellulose (HPMC) K15 was incorporated into optimum formula to form nanoparticle gel of TM and carried out in vivo release study using the Franz Diffusion Cell.
Results: TM nanoparticles was successfully prepared with concentration of CS, SA, and CaCl2 0.01 % (w/v), 0,1 % (w/v), and 0.25 % (w/v) respectively. The particle size, polydispersity index, entrapment efficiency and Zeta potential were found to be 200.47 ± 4.20 nm, 0.27, 35.23 ± 4.55 %, and -5.68 ± 1.80 mV respectively. The result of FTIR spectra indicated TM loaded in the nanoparticles system. In vitro release profile of TM loaded nanoparticle gel showed controlled release and Korsmeyer-Peppas model were found to be the best fit for drug release kinetics.
Conlclusion: Timolol maleat loaded CS/ SA nanoparticles gel was succesfully prepared and could be considered as a promising candidate for controlled TM delivery of IH treatment.
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